3 4 5 dimethylthiazol yl 2 5 diphenyltetrazolium bromide mtt

Manufactured by Merck Group

Sourced in United States

3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT) is a chemical compound used in various laboratory procedures. It serves as a colorimetric assay for assessing cell metabolic activity. The compound undergoes reduction by cellular enzymes, resulting in the formation of insoluble purple formazan crystals, which can be measured spectrophotometrically.

Automatically generated - may contain errors

Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (9 mentions) Verapamil, by Merck Group (7 mentions) Cisplatin, by Merck Group (6 mentions) Doxorubicin, by Merck Group (4 mentions) Paclitaxel, by Merck Group (4 mentions) Triton x 100, by Merck Group (3 mentions) Paraformaldehyde (pfa), by Merck Group (3 mentions) Alexa fluor 488 conjugated goat anti mouse igg secondary antibody, by Thermo Fisher Scientific (3 mentions) Mitoxantrone, by Merck Group (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Colchicine, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) 3h paclitaxel 15 ci mmol, by Moravek Biochemicals (2 mentions) Penicillin streptomycin, by Corning (2 mentions) Trypsin, by Corning (2 mentions) Cisplatin, by Bio-Techne (2 mentions) Docetaxel, by Bio-Techne (2 mentions) Paclitaxel, by Bio-Techne (2 mentions)

14 protocols using 3 4 5 dimethylthiazol yl 2 5 diphenyltetrazolium bromide mtt

Isolation and Characterization of Marine Fungal Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

SAD with a purity of >98% were isolated from metabolites of marine-derived mangrove endophytic fungus and dissolved in DMSO for use at indicated concentrations. GAPDH antibody was purchased from Kangchen Co. (Shanghai, China). Antibodies against c-Jun (#9165), p-c-Jun (#2361), JNK (#9258), p-JNK (#9251), HistoneH3 (#11885), Src (#2109), p-Src (#2101), STAT3 (#9139), p-STAT3 (#9131) were purchased from Cell Signaling Technology (Boston, Massachusetts, USA). Cyclin B1, CDC2 and p-CDC2 (Tyr15) were purchased from Affinity Biosciences (USA). RT Kit was from Thermo Fisher Scientific Inc. (Waltham, MA, USA). MG132, 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT) and other chemicals were purchased from Sigma Chemical Co. (Shanghai, China).

Zhang H., Huang L., Tao L., Zhang J., Wang F., Zhang X, & Fu L. (2018). Secalonic acid D induces cell apoptosis in both sensitive and ABCG2-overexpressing multidrug resistant cancer cells through upregulating c-Jun expression. Acta Pharmaceutica Sinica. B, 9(3), 516-525.

+ Open protocol

+ Expand

Investigating Immune Checkpoint Inhibition in Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

PD0325901 and PD-1 blocking antibody (pembrolizumab) were purchased from Selleck Chemicals. Anti-PD-L1, anti-phospho ERK1/2 and anti-ERK1/2 antibodies were obtained from Santa Cruz Biotechnology. The antibody against GAPDH was purchased from Proteintech. The anti-mouse CD3 and granzyme B antibodies for immunohistochemistry were purchased from Abcam. The anti-human PD-L1 and p-ERK1/2 for immunohistochemistry were obtained from Santa Cruz Biotechnology. The anti-human CD3 and CD8 antibodies for immunohistochemistry were purchased from Zsbio. Dimethyl sulfoxide and 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT) were products of Sigma–Aldrich. DMEM and fetal bovine serum were products of Gibco. Penicillin, streptomycin and trypsin were obtained from Thermo Fisher Scientific. InVivoMab anti-mouse PD-1 (CD279) was purchased from BioXcell.

Luo M., Xia Y., Wang F., Zhang H., Su D., Su C., Yang C., Wu S., An S., Lin S, & Fu L. (2021). PD0325901, an ERK inhibitor, enhances the efficacy of PD-1 inhibitor in non-small cell lung carcinoma. Acta Pharmaceutica Sinica. B, 11(10), 3120-3133.

+ Open protocol

+ Expand

Synthesis and Characterization of Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Amino acids were purchased from Ampebiochem Co. Ltd (Chengdu, China) and Ketoprofen was obtained from energy-chemical Co. Ltd (Shang'hai, China). Uranyl acetate (>99.9%) was obtained from Xi'an Dingtian Chemical Co. Ltd (Xi'an, China). All other chemical reagents and solvents were used as received from commercial sources without further purification.
3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma (St. Louis, MO, USA). TNF-α detection kit was purchased from Nanjing built Biological Technology Co., Ltd. (Nanjing, China). BCG vaccine was purchased from Shanghai Institute of Biological Products Co., Ltd. (Shanghai, China). Trypsin, fetal calf serum and DMEM (Gibco^®) were purchased from Thermo Fisher Scientific (USA). Annexin V-FITC/PI Cell Cycle and Apoptosis Analysis Kit was purchased from Nanjing Keygen Co., Ltd. (Nanjing, China). Deionized distilled water was used for the preparation of all solutions, and the other solvents were HPLC grade.

Chen Z., Xing L., Fan Q., Cheetham A.G., Lin R., Holt B., Chen L., Xiao Y, & Cui H. (2017). Drug-Bearing Supramolecular Filament Hydrogels as Anti-Inflammatory Agents. Theranostics, 7(7), 2003-2014.

+ Open protocol

+ Expand

Glesatinib Characterization and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glesatinib (99% purity as measured by high performance liquid chromatography) was purchased from ChemieTek (Indianapolis, IN). Dulbecco's modified Eagle's Medium (DMEM), bovine serum albumin (BSA), fetal bovine serum (FBS), penicillin/streptomycin and trypsin 0.25% were purchased from Hyclone (GE Healthcare Life Science, Pittsburgh, PA). The monoclonal antibodies for ABCB1 (C219) and GAPDH (MA5-15738), Alexa Fluor 488 conjugated goat anti-mouse IgG secondary antibody were purchased from Thermo Fisher Scientific Inc (Rockford, IL), dimethylsulfoxide (DMSO), 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Triton X-100, 4',6-diamidino-2-phenylindole (DAPI), paraformaldehyde, paclitaxel, doxorubicin, colchicine, cisplatin, verapamil and Ko 143 were purchased from Sigma-Aldrich (St. Louis, MO). [³H]-paclitaxel (15 Ci/mmol) was purchased from Moravek Biochemicals, Inc (Brea, CA). All other chemicals were purchased from Sigma Chemical Co (St. Louis, MO).

Cui Q., Cai C.Y., Gao H.L., Ren L., Ji N., Gupta P., Yang Y., Shukla S., Ambudkar S.V., Yang D.H, & Chen Z.S. (2019). Glesatinib, a c-MET/SMO Dual Inhibitor, Antagonizes P-glycoprotein Mediated Multidrug Resistance in Cancer Cells. Frontiers in Oncology, 9, 313.

+ Open protocol

+ Expand

Methyl-Cantharidimide Cytotoxicity Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methyl-Cantharidimide (MCA) was obtained as a gift from Sihuan Bioengineering Co., Ltd. (Jiangsu, China). Dulbecco's modified Eagle's Medium (DMEM), Bovine serum albumin (BSA), penicillin/streptomycin and 0.25% trypsin, fetal bovine serum (FBS) were purchased from Corning Incorporated (Corning, NY). Dimethylsulfoxide (DMSO), 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), paclitaxel, cisplatin, mitoxantrone were purchased from Sigma-Aldrich (St. Louis, MO). Propidium Iodide (PI) and annexin V were purchased from BD biosciences (San Jose, CA). All the rest chemicals were purchased from Sigma Chemical Co (St. Louis, MO).

Li Y.D., Mao Y., Dong X.D., Lei Z.N., Yang Y., Lin L., Ashby CR J.r., Yang D.H., Fan Y.F, & Chen Z.S. (2020). Methyl-Cantharidimide (MCA) Has Anticancer Efficacy in ABCB1- and ABCG2-Overexpressing and Cisplatin Resistant Cancer Cells. Frontiers in Oncology, 10, 932.

+ Open protocol

+ Expand

Characterization of Nanoparticle-Cell Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

PA (purity > 99%) was sourced from Chengdu Preferred Biological Technology Co., Ltd. (Chengdu, China). d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), 3-(4,5-dimethylthiazolyl)−2,5-diphenyltetrazolium bromide (MTT) and 1-methyl-4-phenylpyridinium ion (MPP⁺) were acquired from Sigma-Aldrich (USA). Calu-3 and SH-SY5Y cell lines were acquired from the American Type Culture Collection (USA). Minimum essential medium (MEM) and fetal bovine serum (FBS) were prepared from Gibco (USA). High-performance liquid chromatography (HPLC)-grade acetonitrile, phosphoric acid, formic acid, and phosphotungstic acid were from Shanghai Aladdin biochemical Technology Co., Ltd. (Shanghai, China). DiO and DiI were purchased from Beyotime (China).

Wu C., Li B., Zhang Y., Chen T., Chen C., Jiang W., Wang Q, & Chen T. (2019). Intranasal delivery of paeoniflorin nanocrystals for brain targeting. Asian Journal of Pharmaceutical Sciences, 15(3), 326-335.

+ Open protocol

+ Expand

Multidrug Resistance Modulation Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

EGCG (purity 97%) was purchased from Hangzhou Hetian Biotechnology Co., Ltd. (Hangzhou, Zhejiang, China). Y₆ (purity 96.87%) was synthesized in our laboratory as previously described²² (link). Doxorubicin, verapamil (VER), dimethylsulfoxide (DMSO) and 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazoliumbromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The ATPase assay kit and membrane vesicles were purchased from BD Biosciences (San Jose, CA, USA). Dulbecco׳s modified Eagle׳s medium (DMEM), fetal bovine serum (FBS), trypsin 0.25% and penicillin/streptomycin were purchased from Hyclone (Thermo Scientific, Logan, UT, USA).

Wen Y., Zhao R., Gupta P., Fan Y., Zhang Y., Huang Z., Li X., Su Y., Liao L., Xie Y.A., Yang D., Chen Z.S, & Liang G. (2018). The epigallocatechin gallate derivative Y6 reverses drug resistance mediated by the ABCB1 transporter both in vitro and in vivo. Acta Pharmaceutica Sinica. B, 9(2), 316-323.

+ Open protocol

+ Expand

Paclitaxel Resistance Mechanism Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

[³H]-Paclitaxel (37.9 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA). Dulbecco modified Eagle medium (DMEM), fetal bovine serum (FBS), phosphate buffer saline (PBS), 10,000 IU/ml penicillin and 10,000 μg/ml streptomycin, and trypsin 0.25% were purchased from Hyclone (Waltham, MA). Monoclonal antibody against GAPDH was purchased from Cell Signaling Technologies (Beverly, MA). The polyclonal antibody PA5-23652 for ABCC10 was obtained from Thermo scientific (Rockford, IL). Monoclonal antibody 3D7G8 against EphB4 was obtained from Life technologies, Invitrogen (New York, NY). NVP-BHG712 was a gift from Novartis (Basel, Switzerland). Cepharanthine was generously donated by Kakenshoyaku Co. (Tokyo, Japan). PAK-104P was a gift from Nissan Chemical Industries (Tokyo, Japan). Paclitaxel, docetaxel, colchicine, mitoxantrone, vincristine, vinblastine and cisplatin were purchased from Tocris Bioscience (Ellisville, MO). 3-(4, 5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and verapamil were obtained from Sigma-Aldrich Co. (St. Louis, MO).

Kathawala R.J., Wei L., Anreddy N., Chen K., Patel A., Alqahtani S., Zhang Y.K., Wang Y.J., Sodani K., Kaddoumi A., Ashby CR J.r, & Chen Z.S. (2014). The small molecule tyrosine kinase inhibitor NVP-BHG712 antagonizes ABCC10-mediated paclitaxel resistance: a preclinical and pharmacokinetic study. Oncotarget, 6(1), 510-521.

+ Open protocol

+ Expand

Synthesis and Evaluation of VKNG-2 Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

VKNG-2 (2-(3-(3-Nitrophenyl) ureido)-N-(4-propylphenyl)benzamide) (Figure 4A) was synthesized as previously described [37 (link)]. Mitoxantrone was bought from Enzo Sciences (Farmingdale, NY, USA) and doxorubicin was acquired from Medkoo Biosciences (Morrisville, NC, USA). SN-38, cisplatin, verapamil, vincristine, 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were procured from Sigma Chemical (St. Louis, MO, USA). Fumitremorgin C (FTC) was obtained as a gracious donation from the Thomas McCloud Laboratory, NIH (Bethesda, MD, USA). Dulbecco’s modified Eagle’s medium (DMEM), 0.25% trypsin, penicillin/streptomycin (P/S), and fetal bovine serum (FBS) were obtained from Hyclone (Waltham, MA, USA). [³H]-mitoxantrone (4 Ci/mmol) was purchased from Moravek Biochemicals, Inc (Brea, CA, USA). Monoclonal antibodies D5V2K (selective against ABCG2), D6A8 (against β-Actin), C33D4 (against PI3K p110β), 9272 (selective against AKT) and secondary anti-rabbit antibody linked with HRP were obtained from Cell Signaling (Danvers, MA, USA). BXP-21 (specific against ABCG2) and Alexa fluor conjugated secondary antibody were obtained from Molecular Probes (Eugene, OR, USA).

Narayanan S., Gujarati N.A., Wang J.Q., Wu Z.X., Koya J., Cui Q., Korlipara V.L., Ashby CR J.r, & Chen Z.S. (2021). The Novel benzamide Derivative, VKNG-2, Restores the Efficacy of Chemotherapeutic Drugs in Colon Cancer Cell Lines by Inhibiting the ABCG2 Transporter. International Journal of Molecular Sciences, 22(5), 2463.

+ Open protocol

+ Expand

Paclitaxel and Associated Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

[³H]-Paclitaxel (25.7 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA) Dulbecco modified Eagle medium (DMEM), Iscove’s DMEM, fetal bovine serum (FBS), phosphate buffer saline (PBS), 10,000 IU/ml penicillin and 10,000 μg/ml streptomycin, and trypsin 0.25% were purchased from Hyclone (Waltham, MA). Monoclonal antibody against GAPDH was purchased from Cell Signaling Technologies (Beverly, MA). Antibody D-19 against ABCC10 was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Monoclonal antibody Ab81 against c-Kit and monoclonal antibody D12E12 against phospho-c-Kit (p-c-Kit) were obtained from Cell Signaling Technologies (Beverly, MA). Masitinib was a gift from AB Science (Paris, France). Cepharanthine was generously given by Kakenshoyaku Co. (Tokyo, Japan). PAK-104P was a gift from Nissan Chemical Industries (Tokyo, Japan). Paclitaxel, docetaxel, vincristine, vinblastine and cisplatin were purchased from Tocris Bioscience (Ellisville, MO). Boron-dipyrromethene (BODIPY) FL (fluorescent) Paclitaxel was purchased from Life technologies, Invitrogen (New York, NY). 3-(4, 5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and verapamil were obtained from Sigma-Aldrich Co. (St. Louis, MO).

Kathawala R.J., Sodani K., Chen K., Patel A., Abuznait A.H., Anreddy N., Sun Y.L., Kaddoumi A., Ashby CR J.r, & Chen Z.S. (2014). Masitinib Antagonizes ATP-Binding Cassette Subfamily C Member 10-Mediated Paclitaxel Resistance: A Preclinical Study. Molecular cancer therapeutics, 13(3), 714-723.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!