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3 protocols using potassium phosphate monobasic anhydrous kh2po4

1

Mitochondrial Respiration Assay

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Sodium pyruvate, malate, rotenone, carbonyl cyanide p-trifluoromethoxy-phenylhydrazone (FCCP), magnesium chloride (MgCl2), sucrose, mannitol, EGTA, EDTA, bovine serum albumin (BSA), glycerol, HEPES potassium salt, Triton-X, Tris Buffered Saline with Tween 20 (TBS-T), Tris HCl, NaCl, KCl, potassium phosphate monobasic anhydrous (KH2PO4), were obtained from Sigma Aldrich (St. Louis, MO); oligomycin was obtained from Enzo Life Sciences (Farmingdale, NY) and protease inhibitors (complete Mini Preotease Inhibitor Cocktail tablet) were from Roche Diagnostics (Indianapolis, IN).
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2

Mitochondrial Respiration Assay Protocol

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Sodium pyruvate, malate, rotenone, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), magnesium chloride (MgCl2), sucrose, mannitol, EGTA, EDTA, bovine serum albumin (BSA), glycerol, HEPES potassium salt, Triton-X, Tris Buffered Saline with Tween 20 (TBS-T), Tris HCl, NaCl, KCl, potassium phosphate monobasic anhydrous (KH2PO4), and DMSO were obtained from Sigma Aldrich (St. Louis, MO). The citric acid (anhydrous) was from Fisher Scientific (Omaha, NE). The oligomycin was obtained from Enzo Life Sciences (Farmingdale, NY) and the protease inhibitors (Complete Mini Preotease Inhibitor Cocktail tablet) were from Roche Diagnostics (Indianapolis, IN). U-74389G was obtained from Cayman Chemical (Ann Arbor MI).
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3

Mitochondrial Function Assay Protocol

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Sodium pyruvate, malate, rotenone, carbonyl cyanide p-trifuoromethoxyphenylhydrazone (FCCP), magnesium chloride (MgCl2), sucrose, mannitol, EGTA, EDTA, bovine serum albumin (BSA), glycerol, HEPES potassium salt, Triton-X, Tris Buffered Saline with Tween 20 (TBS-T), Tris HCl, NaCl, KCl, potassium phosphate monobasic anhydrous (KH2PO4), and DMSO were obtained from Sigma Aldrich (St. Louis, MO). The citric acid (anhydrous) was from Fisher Scientific (Omaha, NE). The oligomycin was obtained from Enzo Life Sciences (Farmingdale, NY) and the protease inhibitors (Complete Mini Preotease Inhibitor Cocktail tablet) were from Roche Diagnostics (Indianapolis, IN). U-74389G or Methylated Tirilazad (TZ) was obtained from Cayman Chemical (Ann Arbor MI).
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