The largest database of trusted experimental protocols

37 protocols using sodium tungstate

1

Analyzing Protonema Growth in Physcomitrella

Check if the same lab product or an alternative is used in the 5 most similar protocols
Explants from P. patens (Hewd) B.S.G protonema were cultivated on solid Knop medium (0.8% Agar) [51 (link)] covered with sterile cellophane discs. Plants were cultivated in a growth chamber at 21°C with 16/8h light/dark period. Light intensity was 30 μmol m-2 s-1.
To analyse the effect of sodium tungstate on P. patens protonema growth, seven day-old protonema pieces were transferred from simple Knop medium to the same medium supplemented with different concentrations of sodium tungstate (Sigma-Aldrich). The plants were grown for seven days under treatment. At the day of the transfer (day 0) and seven days later when the experiment ended (day 7) the Petri dishes with the protonema cultures were photographed with a Nikon Colpix5000 camera. To analyse the effect of high nitrate concentration (8.4 mM Ca(NO3)2) on P. patens growth, the same assay was performed adding nitrate to Knop media alone and to Knop media supplemented with 30 μM sodium tungstate. The area covered by each plant at day zero and day seven were measured using ImageJ software (http://rsb.info.nih.gov/ij/). The relative growth rate was calculated using the following formula: (ln af—ln a0)t-1 [52 ] where af and a0are the areas occupied by the plant at final day of growth (day 7) and at the beginning of the experiment (day 0) and t is the duration of growth equal to 7 days.
+ Open protocol
+ Expand
2

Antioxidant Assays Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Analytical grade chemicals used: sodium carbonate, sodium nitrite, dosium dihydrogen, hydrogen peroxide, ferrous chloride, 2-deoxyribose, potassium ferricyanide, sulphuric acid were bought from Merck. 1,1-diphenyl-2-picryl-hydrazyl, potassium persulphate, 2-ethylbenzothiazoline sulfonic acid, rutin, nitro blue, Folin-Ciocalteu’s reagent, phenazine methosulphate, trichloroacetic-acid and tetrazolium were obtained from Sigma Chemicals Co., St. Louis USA. Oxidized glutathione (GSSG), (DTNB), glutathione (GSH),1,2-dithio-bis-nitroLbenzoic-acid (DTNB), glucose-6-phosphate, thiobarbituric acid (TBA), trichloroacetic acid (TCA), sodium tungstate, perchloric acid (PCA), 2,6-dichlorophenolindophenol, reduced glutathione (GSH), sodium hydroxide, reduced nicotinamide adenine dinucleotide phosphate (NADPH), sodium tungstate, glucose-6-phosphate, rutin, catechin, gallic acid, caffeic acid, apigenin, quercetin, myricetin, and kampferol were bought from Sigma Chemicals Co., USA were used.
+ Open protocol
+ Expand
3

Guava Leaf DPPH Antioxidant Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh guava leaves were collected from farms in Shilou town of Guangzhou, Guangdong. This specie was locally called as pearl guava leaves. Samples were transferred on the same day to Pharmaceutical Engineering Lab in Guangdong Pharmaceutical University and were dried in 60°C then stored until analysis. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) was purchased from Sigma Chemical Co. salicylic acid, sodium tungstate, sodium nitrite, EtOH, FeSO4, etc., All chemicals used were of analytical grade and were purchased from a local Chemical Co. (Congyuan Co., Ltd, Guangzhou).
+ Open protocol
+ Expand
4

Labeling Insect Pupae with Chemicals

Check if the same lab product or an alternative is used in the 5 most similar protocols
An aqueous solution (1.0 M) of sodium tungstate (Sigma-Aldrich, St. Louis, MO, USA) or an aqueous solution (30%) of FB28 (fluorescent brightener 28 disodium salt) (Sigma-Aldrich, St. Louis, MO, USA) was injected into the abdomen of pupae within 12 h post-pupation. The injection volume was made variable for sodium tungstate (see Section 3) but was 2.0 μL for FB28. The pupae were removed from the wall of the container before injection, and injection was performed using an Ito (Terumo) microsyringe (MS-25) (Fuji, Shizuoka, Japan). After injection, they were placed back to the original position on the wall using adhesive tape.
+ Open protocol
+ Expand
5

Synthesis of BaMoO4:Sm3+/Dy3+ Phosphor

Check if the same lab product or an alternative is used in the 5 most similar protocols
Starting materials: barium acetate ((CH3COO)2Ba, Sigma-Aldrich), sodium tungstate (Na2MoO4·2H2O, Sigma-Aldrich), dysprosium(iii) nitrate (Dy(NO3)3·5H2O, Dy3+), and samarium(iii) nitrate (Sm(NO3)3·5H2O, Sm3+).
10 mmol of (CH3COO)2Ba was dissolved in beaker “A” containing 100 mL of distilled water. In beaker “B”, 10 mmol of Na2MoO4·2H2O was dissolved in 10 mL distilled water (Fig. 1). The solution that was completely dissolved in beaker “B” was slowly poured into the stirring beaker “A” and remained there for about 20 minutes. After that, powder was recovered using a centrifuge (4000 rpm, 5 min), and the powder was prepared by rinsing with distilled water 3 times to remove any remaining sodium. The white powder was dried in an oven at 80 °C for 16 hours. The phosphor was synthesized with BaMoO4 as a host, the precursor was made by simultaneously adding Dy(NO3)3·5H2O and Er(NO3)3·5H2O or Sm(NO3)3·5H2O to beaker “A”. The BaMoO4:[Sm3+]/[Dy3+] phosphor was synthesized. Rare earth [RE] ions of Dy3+ were fixed at 0.05 mmol, and the amount of added Sm3+ was changed ([Sm3+]/[Dy3+] ∼ 0.1, 0.2, 0.3, 0.4, 0.6, 0.8, 1).21 (link)
+ Open protocol
+ Expand
6

Preparation of Luria Bertani Broth for Bacterial Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols
Luria Bertani broth was procured from Himedia Laboratories Pvt. Ltd, India and Sodium tungstate was of analytical grade, purchased from Sigma-Aldrich.
+ Open protocol
+ Expand
7

Comparative Analysis of U. tomentosa Products

Check if the same lab product or an alternative is used in the 5 most similar protocols
A total of 32 U. tomentosa products were purchased in the Spanish Herbalists in Madrid and in the Costa Rican Macrobioticas in San Jose. The products were classified upon their labeled material (bark or leaves) and presentation (capsules, tablets, syrups, or tea bags). In order to be able to obtain valid comparable results, the total of 18 bark capsule products were selected, corresponding to 6 from Costa Rica (CR-1 to CR-6) and 12 from Spain (SP-1 to SP-12). Noteworthy, most products did not specify their composition, one exception was SP-2 whose label specified 15% of polyphenolic bark extract per capsule. The material studied was preserved in a fresh place at room temperature. Solvents such as ethanol, methanol, and acetonitrile were purchased from Baker (Center Valley, PA, USA). Reagents such as AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), sodium molibdate, gallic acid, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), fluorescein, and sodium tungstate were provided by Sigma-Aldrich (St. Louis, MO, USA).
+ Open protocol
+ Expand
8

Synthesis of Styrene-based Polymers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Styrene, vinylbenzyl chloride, acrylonitrile, N,N-dimethylformamide (DMF), sodium tungstate, benzoyl peroxide (BPO), and trimethylamine (TMA) were purchased from Sigma Aldrich (Yongin-si, Korea). All the chemicals used in the present investigation are of reagent grade.
+ Open protocol
+ Expand
9

Electrochemical Characterization of Polymer Composites

Check if the same lab product or an alternative is used in the 5 most similar protocols
PEI (MW 72,000),
potassium ferrocyanide (K4[Fe(CN)6]·3H2O), silver nitrate (AgNO3), sodium tungstate (Na2WO4), PDDA (MW approx. 20,000), and potassium ferricyanide
(K3[Fe(CN)6]) were purchased from Sigma Aldrich
Corp. (USA). The chemicals were of analytical grade and used as it
is. All the buffer solutions/electrolytes were made from highly purified
water from ELGA PURELAB. The following electrolyte solutions were
utilized for the electrochemical studies: 0.1 M Na2SO4 (pH 2.0, 2.5, and 3.0), 0.1 M Na2SO4/20 mM CH3COOH (pH 3.5, 4.0, 4.5, and 5.0), and 0.1 M
Na2SO4/20 mM NaH2PO4 (pH
5.5 and 6.0). 0.1 M NaOH or 0.1 M H2SO4 was
used to adjust the pH of respective buffer solutions. In addition,
1 mM solution of potassium ferrocyanide and potassium ferricyanide
[1:1]/0.1 M KCl was used for electrochemical impedance experiments.
+ Open protocol
+ Expand
10

Cytotoxicity and Apoptosis Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metal salts were purchased from Sigma, (St. Louis, MO, USA): 10ÿ PBS (D1408), sodium tungstate (379751), sodium acetate (59929), nickel acetate (379883), and cobalt acetate (399973). Test kits were purchased from Promega (Madison, WI, USA): Multitox Cytotoxicity Assay (G9270), and Caspase Glo 3/7 Assay (G8091). Tissue culture supplies (unless otherwise noted) were purchased from Fisher Scientific (Pittsburg, PA, USA): Hyclone molecular biology grade water (SH30538), Roswell Park Memorial Institute (RPMI-1640) cell culture medium supplemented with l-glutamine (SH30027FS), Neuronal Growth Factor (NGF) 2.5S natural mouse (356004), rat tail Collagen I (CB-40236), 100ÿ penicillin/streptomycin solution (SV30010), equine serum (donor herd defined and heat inactivated, SH3007403HI), fetal bovine serum (FBS-heat inactivated, SH3008803HI), and nitric acid (PN A467-1 Optima grade). Buffer RLT (PN-79216) was purchased from Qiagen (Germantown, MD, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!