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Rfp 5f8

Manufactured by Proteintech
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Sourced in France

The RFP (5F8) is a laboratory equipment product from Proteintech. It is a recombinant protein that can be used for various research applications. The core function of the RFP (5F8) is to serve as a research tool, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Eif3d, by Santa Cruz Biotechnology (2 mentions) Pdest17 vector, by Thermo Fisher Scientific (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Mg132 s2619, by Selleck Chemicals (1 mentions) Gapdh 0411, by Santa Cruz Biotechnology (1 mentions) Cyclin d3 dcs22, by Cell Signaling Technology (1 mentions) C myc 9e10, by Developmental Studies Hybridoma Bank (1 mentions) Forskolin, by Apexbio (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Sb203580, by LC Laboratories (1 mentions)

Close spelling variants of this product

Anti-RFP (5F8) Rat anti-RFP 5F8 antibody Rat anti-RFP (5F8)

3 protocols using rfp 5f8

1

Comprehensive Antibody and Reagent Source

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The following antibodies K19 (A53-B/A2), GSK3β (E-11), pGSK3β (F-2), PARP-1 (F-2), β-actin (C4), and GAPDH (0411) were from Santa Cruz Biotechnology (Santa Cruz, CA); cyclin D3 (DCS22) and GSK3β (D5C5Z) were from Cell Signaling Technology (Danvers, MA); GFP (3H9) and RFP (5F8) were from Chromotek (Islandia, NY); cyclin D3 (26755-1-AP) was from Proteintech (Rosemont, IL); and anti-tubulin (12G1), anti-GFP (1D2), and c-Myc (9E10) were from Developmental Studies Hybridoma Bank (Iowa City, IA). Forskolin was from ApexBio (#B1421) (Houston, TX), LiCl (# EM-LX0331) and DMSO were from VWR Life Sciences (Carlsbad, CA), LY294002 was from AmBeed (#A133122) (Arlington Hts, IL), SB 203580 (#S-3400) and SB 202190 (S-1700) were from LC Laboratories (Woburn, MA), okadaic acid (459616) was from EMD Millipore (Billerica, MA), cycloheximide (#66-81-9) was from Sigma-Aldrich (St Louis, MO), and MG132 (S2619) was from Selleck Chemicals.
Sharma P., Tiufekchiev S., Lising V., Chung S.W., Suk J.S, & Chung B.M. (2021). Keratin 19 interacts with GSK3β to regulate its nuclear accumulation and degradation of cyclin D3. Molecular Biology of the Cell, 32(21), ar21.
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2

Antibody Production against CERES Protein

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Proteins were separated by SDS-polyacrylamide gel electrophoresis, blotted to nitrocellulose membranes and analysed with specific antibodies against HA (Roche), Flag (Sigma Aldrich), GST (Santa Cruz Biology), GFP (Roche), RFP (5F8, Chromotek), Myc (Clone 4A6, Merck), eIF4A (St. John’s laboratory), eIF3D (sc-28856, Santa Cruz), AteIF4E and AteIF(iso)4E (kindly provided by Dr. J. L. Gallois, INRA, France) and TaeIF4E (kindly provided by Dr. Karen Browning, University of Texas, USA).
The C-terminal part of CERES (base pairs 1471-1794) was cloned into pDEST17 vector (Invitrogen) and transformed into E. coli strain BL21. Cells were induced for protein expression by adding 0.1 mM IPTG and the proteins were separated by SDS-polyacrylamide gel electrophoresis. Following SDS-PAGE, the gels were incubated in cold 2 M KCl until the protein bands became opaque. The band corresponding to Ct-CERES was excised from the gel. This band was used to immunise rabbits for antiserum production by Pineda Antibody Services (Germany).
Toribio R., Muñoz A., Castro-Sanz A.B., Merchante C, & Castellano M.M. (2019). A novel eIF4E interacting protein that forms non-canonical translation initiation complexes. Nature plants, 5(12), 1283-1296.
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3

Antibody Production against CERES Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated by SDS-polyacrylamide gel electrophoresis, blotted to nitrocellulose membranes and analysed with specific antibodies against HA (Roche), Flag (Sigma Aldrich), GST (Santa Cruz Biology), GFP (Roche), RFP (5F8, Chromotek), Myc (Clone 4A6, Merck), eIF4A (St. John’s laboratory), eIF3D (sc-28856, Santa Cruz), AteIF4E and AteIF(iso)4E (kindly provided by Dr. J. L. Gallois, INRA, France) and TaeIF4E (kindly provided by Dr. Karen Browning, University of Texas, USA).
The C-terminal part of CERES (base pairs 1471-1794) was cloned into pDEST17 vector (Invitrogen) and transformed into E. coli strain BL21. Cells were induced for protein expression by adding 0.1 mM IPTG and the proteins were separated by SDS-polyacrylamide gel electrophoresis. Following SDS-PAGE, the gels were incubated in cold 2 M KCl until the protein bands became opaque. The band corresponding to Ct-CERES was excised from the gel. This band was used to immunise rabbits for antiserum production by Pineda Antibody Services (Germany).
Toribio R., Muñoz A., Castro-Sanz A.B., Merchante C, & Castellano M.M. (2019). A novel eIF4E interacting protein that forms non-canonical translation initiation complexes. Nature plants, 5(12), 1283-1296.
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