C57bl 6j rj h 2b mice

Eight to 10 week-old C57BL/6J Rj (H-2b) mice were purchased from Janvier (Le Genest-St. Isle, France). The syngeneic tumorigenic murine pancreatic carcinoma cell line Panc02 was cultured in RPMI 1640 10% FCS 100 units/mL penicillin (Invitrogen), 100 μg/mL streptomycin and were tested negative for mycoplasma contamination.

Female, 5−6-week-old C57BL/6JRj (H-2^b) mice were purchased from Janvier Laboratory (Saint-Berthevin, France). The mice were maintained under specific pathogen-free conditions at Institut Pasteur animal facilities and were immunized at 7−10 weeks of age via intramuscular route or intravenous route with 5 × 10⁷ TU of lentiviral vector or 1 × 10⁷ IGU of Ad5 in 50 μl of vector diluted in PBS. C57BL/6-Tg(TcraTcrb)1100Mjb/Crl female OT-1 mice were purchased from Charles River (Écully, France). Female CD Sprague-Dawley rats (201–225 g) were purchased from Charles River Laboratories (Saint Germain Nuelles, France). Rats were maintained in the conventional animal facilities of Sciensano and were immunized at 11–14 weeks of age via an intramuscular route with 5 × 10⁷ TU of lentiviral vector or 1 × 10⁷ IGU of Ad5 in 50 μl of vector diluted in PBS. All the studies were conducted in accordance with the European and French directives (Directive 86/609/CEE and Decree 87-848 of 19 October 1987), after approval by the Institut Pasteur Safety, Animal Care and Use Committee, under local ethical committee protocol agreement (APAFIS#19863-2019031917064954 and APAFIS#20289-2019060616332025) or after approval by the Ethical Commission of Sciensano (ethical committee project agreement 20180910-01).