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Anti foxp3 fjk 16a

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Anti-FoxP3 (FJK-16a) is a monoclonal antibody that recognizes the transcription factor FoxP3. FoxP3 is a key regulator of the development and function of regulatory T cells (Tregs). The antibody can be used for the identification and analysis of FoxP3-expressing cells in flow cytometry applications.

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Lab products found in correlation

Anti phospho stat3 py705, by Thermo Fisher Scientific (2 mentions) Anti cd73 ty 11.8, by BioLegend (1 mentions) Fitc annexin 5 staining kit, by BioLegend (1 mentions) Zombie violet viability dye, by BioLegend (1 mentions) Fixation permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Aria 3, by BD (1 mentions) Facscalibur, by BD (1 mentions)

3 protocols using anti foxp3 fjk 16a

1

Multiparametric Flow Cytometry Analysis

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Cells were stained with anti-CD4 (RM4-5), anti-IL-17A (eBio17B7), anti-IFN-γ (XMG1.2), anti-Thy1.1 (HIS51), anti-CD45.1 (A20), anti-CD25 (PC61.5), anti-TCRβ (H57-597), anti-phospho-STAT3 (pY705) and anti-FoxP3 (FJK-16a) Abs (all Abs from eBioscience). Cells were acquired using a LSRII (BD Biosciences) flow cytometer and analyzed using a FlowJo software (Treestar, Ashland, OR).
Do J.S., Visperas A., Sanogo Y.O., Bechtel J.J., Dvorina N., Kim S., Jang E., Stohlman S.A., Shen B., Fairchild R.L., Baldwin WM I.I.I., Vignali D.A, & Min B. (2015). An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell suppressive function and therapeutic efficacy. Mucosal immunology, 9(1), 137-145.
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-CD4 (GK1.5), anti-CD25 (PC-61), anti-Vα3.2 (RR3-16), anti-CD45.2 (104), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-ICOS (C398.4A), anti-PD-1 (29F.1A12) and anti-CD73 (TY/11.8) were from BioLegend. Anti-Foxp3 (FJK-16a) was from eBioscience. Cell sorting and analyses were performed on ARIA III, FACS CALIBUR, LSRII, and CANTO (BD). For detection of apoptosis, FITC Annexin V staining kit and Zombie Violet viability dye were used from BioLegend. For intracellular staining cells were fixed and permeabilized using Fixation/Permeabilization buffers from eBioscience and BD according to manufacturers’ manual.
Jones A., Opejin A., Henderson J.G., Gross C., Jain R., Epstein J.A., Flavell R.A, & Hawiger D. (2015). Peripherally induced tolerance depends on pTreg cells that require Hopx to inhibit intrinsic IL-2 expression. Journal of immunology (Baltimore, Md. : 1950), 195(4), 1489-1497.
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3

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with anti-CD4 (RM4-5), anti-IL-17A (eBio17B7), anti-IFN-γ (XMG1.2), anti-Thy1.1 (HIS51), anti-CD45.1 (A20), anti-CD25 (PC61.5), anti-TCRβ (H57-597), anti-phospho-STAT3 (pY705) and anti-FoxP3 (FJK-16a) Abs (all Abs from eBioscience). Cells were acquired using a LSRII (BD Biosciences) flow cytometer and analyzed using a FlowJo software (Treestar, Ashland, OR).
Do J.S., Visperas A., Sanogo Y.O., Bechtel J.J., Dvorina N., Kim S., Jang E., Stohlman S.A., Shen B., Fairchild R.L., Baldwin WM I.I.I., Vignali D.A, & Min B. (2015). An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell suppressive function and therapeutic efficacy. Mucosal immunology, 9(1), 137-145.
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