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4 2 hydroxyethyl piperazine 1 ethanesulfonic acid

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4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid is a commonly used chemical compound in laboratory settings. It is a zwitterionic organic compound that functions as a buffer, maintaining a stable pH environment in aqueous solutions. This compound is frequently employed in biological and biochemical applications to maintain optimal pH conditions for various experiments and processes.

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Lab products found in correlation

Triton x 100, by Merck Group (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Glutaraldehyde, by Merck Group (3 mentions) Ascorbic acid, by Merck Group (3 mentions) Acetonitrile, by Merck Group (3 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Insulin, by Merck Group (2 mentions) Chloroform, by Merck Group (2 mentions) N 2 hydroxyethylpiperazine n 2 ethanesulfonic acid, by Merck Group (2 mentions) Imidazole, by Merck Group (2 mentions) Sodium citrate, by Merck Group (2 mentions) Bis 2 ethylhexyl sebacate, by Merck Group (2 mentions) Dichloromethane, by Merck Group (2 mentions) Tetrahydrofuran, by Merck Group (2 mentions) 1 2 disteroyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 550 ammonium salt in chloroform dspe peg, by Avanti Polar Lipids (2 mentions) Piperazine n n bis 2 ethanesulfonic acid, by Merck Group (2 mentions) Hepes, by Merck Group (2 mentions) Cucurbit 6 uril hydrate, by Merck Group (2 mentions) Tris hydrochloride, by Merck Group (2 mentions) α hl from staphylococcus aureus, by Merck Group (2 mentions)

Close spelling variants of this product

Piperazine (58 citations)

27 protocols using 4 2 hydroxyethyl piperazine 1 ethanesulfonic acid

1

Cell Line Cultivation Conditions

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T47D-KBluc and MDA-MB-453 cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). The MCF7 and HeLa cell lines were a gift from Dr Sandra Dunn (Division of Hematology and Oncology, Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada). TamR cell lines TamR3 and TamR6 were kindly provided by Dr Euphemia Leung (University of Auckland, New Zealand) [45 (link)]. Cells were cultured at 37°C in a humidified incubator with 5% CO2. The cell lines were maintained in the following culture media: for MCF7, phenol red–free RPMI 1640 (Gibco/Life Technologies, Grand Island, NY, USA) supplemented with 10% foetal bovine serum (FBS) (Gibco/Life Technologies); for T47D-KBluc, phenol red–free RPMI 1640 containing 4.5 g/L glucose (Sigma-Aldrich, St Louis, MO, USA), 10 mM 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (Sigma-Aldrich), pH 7.5, 1 mM sodium pyruvate (Life Technologies), 0.2 U/ml insulin (Sigma-Aldrich) and 10% FBS; for MDA-MB-453 and HeLa, Dulbecco’s modified Eagle’s medium (HyClone Laboratories/GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% FBS (Gibco/Life Technologies); and for TamR3 and TamR6, phenol red–free RPMI 1640 containing 10% charcoal-stripped serum (CSS) (Gibco/Life Technologies) and 1 μM tamoxifen (Sigma-Aldrich).
Singh K., Munuganti R.S., Leblanc E., Lin Y.L., Leung E., Lallous N., Butler M., Cherkasov A, & Rennie P.S. (2015). In silico discovery and validation of potent small-molecule inhibitors targeting the activation function 2 site of human oestrogen receptor α. Breast Cancer Research : BCR, 17(1), 27.
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2

Antimicrobial Susceptibility Assay Protocol

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Mueller-Hinton broth (MHB), Mueller-Hinton agar (MHA) and MRS powder were obtained from AoBoX (China). Bovine serum albumin (BSA), Triton X-100, polymyxin B, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), dimethyl sulfoxide (DMSO), BODIPY-TR-cadaverine (BC), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 3,3′-dipropylthiadicarbocyanine (disc3–5), ethanol (analytical grade, 99%), tertiary butanol (analytical grade, 99%), acetone (analytical grade, 99%), glutaraldehyde (synthetic grade, 50% in H2O), lipoteichoic acid (LTA) from S. aureus and lipopolysaccharide (LPS) from E. coli were purchased from Sigma-Aldrich (China). Phosphate-buffered saline (PBS) solution, sodium chloride, potassium chloride, ammonium chloride, calcium chloride, zinc chloride, magnesium chloride, and ferric chloride were purchased from Kermel (China). Glucose (analytical grade) was obtained from Zhiyuan (Guangdong, China). DMEM phenol red-free medium and fetal bovine serum were purchased from Gibco (Beijing, China). All these reagents were used according to the required concentration range.
Chou S., Li Q., Nina Z., Shang L., Li J., Li J., Wang Z, & Shan A. (2020). Peptides With Triplet-Tryptophan-Pivot Promoted Pathogenic Bacteria Membrane Defects. Frontiers in Microbiology, 11, 537.
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3

Proteomic Sample Preparation Procedure

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DSSO, Dulbecco’s modified Eagle’s medium (DMEM), foetal bovine serum (FBS), l-glutamine, penicillin, streptomycin and phosphate-buffered saline (PBS) were obtained from Thermo Fisher Scientific (Les Ulis, France). Formic acid (FA), HPLC grade water, trifluoroacetic acid (TFA), acetonitrile (ACN), methanol, ethanol, acetone and trichloroacetic acid were all purchased from Biosolve (Dieuze, France). dl-Dithiothreitol (DTT), iodoacetamide (IAA), chloroform, dimethyl sulfoxide (DMSO), ammonium bicarbonate (AB), 4-(2-hydroxyethyl)piperazine-1-ethane sulfonic acid, N-(2-hydroxyethyl)piperazine-N-(2-ethane sulfonic acid) (HEPES), sodium chloride (NaCl) and magnesium chloride (MgCl2) were obtained from Sigma-Aldrich. Tris was purchased from Bio-Rad (Steenvoorde, France). Extraction Illustra triplePrep Kit was from GE Healthcare. LysC/trypsin was obtained from Promega (Charbonnières-les-Bains, France). Amicon centrifugal filters and C18 ZipTip pipette tips were from Merck Millipore (Merck KGaA, Darmstadt, Germany).
Cardon T., Franck J., Coyaud E., Laurent E.M., Damato M., Maffia M., Vergara D., Fournier I, & Salzet M. (2020). Alternative proteins are functional regulators in cell reprogramming by PKA activation. Nucleic Acids Research, 48(14), 7864-7882.
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4

HPLC-based Chemical Compound Identification

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Methanol HPLC grade (99.9%), sodium dodecyl sulphate (SDS), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), tris(hydroxymethyl)aminomethane (TRIS), and thiourea were purchased from Sigma-Aldrich (Steinheim, Germany). Dimethyl sulfoxide (DMSO) and sodium hydroxide (NaOH) were bought from POCH (Gliwice, Poland). Ultrapure water was purified by Millipore Direct-Q 3 UV Water Purification System (Millipore Corporation, Bedford, MA, USA).
Ciura K., Fedorowicz J., Andrić F., Žuvela P., Greber K.E., Baranowski P., Kawczak P., Nowakowska J., Bączek T, & Sączewski J. (2019). Lipophilicity Determination of Antifungal Isoxazolo[3,4-b]pyridin-3(1H)-ones and Their N1-Substituted Derivatives with Chromatographic and Computational Methods. Molecules, 24(23), 4311.
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5

Functionalization of Silk Fabric with Sodium Meta-Arsenite

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Sodium meta-arsenite NaAsO2 was obtained from Sigma-Aldrich, while HCl, NaOH, KNO3, and Cu (NO3) ∙ 3H2O obtained from Merck. 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid (call MES hydrate) & 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)(call HEPES), used for pH buffering, were obtained from Sigma-Aldrich. Milli-Q Academic system, Millipore produced ultrapure water.
The Silk Fabric (SF) provided by Tsiakiris Georgios Silk Company, Alexandroupoli, Greece. Sodium carbonate (Na2CO3) purchased from Riedel de Haën. The coupling agent 3-(chloropropyl)trimethoxysilane was provided by Fluka. Methanol and ethanol purchased from Merck and diethyl ether from Sigma Aldrich.
All reagents were of analytical reagent grade purity, and all solutions prepared using deionized water obtained with a Milli-Q system with a conductivity of 18.2 μS cm−1.
Georgiou Y., Rapti S., Mavrogiorgou A., Armatas G., Manos M.J., Louloudi M, & Deligiannakis Y. (2020). A Hybrid {Silk@Zirconium MOF} Material as Highly Efficient AsIII-sponge. Scientific Reports, 10, 9358.
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6

Comprehensive Enzymatic Assay Protocol

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Nicotinamide (N0636), streptozotocin (S0130), ethylenediaminetetraacetic acid (EDS), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (H3375), imidazole (I0250), sodium dodecyl sulfate (L3771), ascorbic acid (A7506), malachite green (M6880), Tween® 20 (P1379), Tris-HCl (T3253), MgCl2 (M9272), glucose 6-phosphate (G7879), fructose 1,6-bisphosphate (F6803), adenosine 5′-monophosphate (A2252), 4-nitrophenyl α-D-glucopyranoside (N1377), and intestinal acetone powders from rat (I1630) were purchased from Sigma-Aldrich (Steinheim, Germany). Ammonium molybdate (AT0330-5) was bought from Tecsiquim (Mexico City, Mexico).
Andrade-Cetto A., Espinoza-Hernández F., Mata-Torres G, & Escandón-Rivera S. (2021). Hypoglycemic Effect of Two Mexican Medicinal Plants. Plants, 10(10), 2060.
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7

Liposomal Doxorubicin Formulation

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Dipalmitoylphosphatidylcholine (DPPC); hydrogenated soy phosphatidylcholine (HSPC); 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000) were kind gifts from Lipoid GmbH (Germany). 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine (MSPC) were purchased from Avanti Polar lipid (Alabama, USA). Cholesterol, isopropanol, chloroform, methanol, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), and doxorubicin hydrochloride (Dox) were purchased from Sigma (UK). [14C] doxorubicin hydrochloride (14C-Dox) were purchased from PerkinElmer (UK). All chemical substances and solvents were used without further purification.
Al-Jamal W.T, & Kostarelos K. (2022). Mild hyperthermia accelerates doxorubicin clearance from tumour-extravasated temperature-sensitive liposomes. Nanotheranostics, 6(3), 230-242.
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8

Enzymatic Assay for Glycosyltransferase Activity

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l-Lactic dehydrogenase
(LDH, L2500), PK (P1506), cytidine 5′-monophosphate
(CMP, C1006), bovine serum albumin (BSA, A3059), N-(2-hydroxyethyl) piperazine-N′-(2-ethanesulfonic
acid), 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES,
H3375), CMP-sialic acid (CMP-Neu5Ac, C1006), uridine 5′-diphosphate
disodium (UDP, 94330), phosphoenolpyruvic acid monopotassium salt
(PEP-K, 860077), adenosine 5′-triphosphate disodium salt trihydrate
(ATP, 10519979001), β-nicotinamide adenine dinucleotide, and
reduced disodium salt hydrate (NADH, 10128023001) were all purchased
from Sigma-Aldrich. Other substrates, cytidine diphosphate (CDP, NC09380),
guanosine diphosphate (GDP, FG152484), galacto-N-biose
(OA01686), lacto-N-biose (OL01707), T-Bn (OB05157),
GalNAc-Ser (MA07378), GDP-l-fucose disodium salt (GDP-Fuc,
MG01912), UDP-α-d-galactose disodium (UDP-Gal, MU58246),
and the inhibitor Soyasaponin1 (FS65454) were all purchased from Biosynth
(Carbosynth).
Nashed A, & Naidoo K.J. (2024). Universal Glycosyltransferase Continuous Assay for Uniform Kinetics and Inhibition Database Development and Mechanistic Studies Illustrated on ST3GAL1, C1GALT1, and FUT1. ACS Omega, 9(15), 17518-17532.
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9

Gold Nanoparticle Functionalization Protocol

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Gold chloride hydrate and CF300-Cu-50 transmission electron microscopy (TEM) grids were purchased from Electron Microscopy Services. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), O-(2-carboxyethyl)-O′-(2-mercaptoethyl)heptaethylene glycol (HEG), sodium citrate, calcein, sodium phosphate, Triton X-100, CL-4B sepharose, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxy sulfo-succinimide sodium salt (NHS) were purchased from Sigma-Aldrich. Polystyrene disposable cuvettes were purchased from VWR. Millipore deionized water was used in all experiments without further treatment. All reagents and solvents were used as is without further purification.
Zheng Z., Saar J., Zhi B., Qiu T.A., Gallagher M.J., Fairbrother D.H., Haynes C.L., Lienkamp K, & Rosenzweig Z. (2018). Structure–Property Relationships of Amine-rich and Membrane-Disruptive Poly(oxonorbornene)-Coated Gold Nanoparticles. Langmuir : the ACS journal of surfaces and colloids, 34(15), 4614-4625.
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10

Vanadium Coordination Compounds Synthesis

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Water was deionized through the Millipore
Milli-Q Academic system or purchased from Sigma-Aldrich (LC-MS grade).
VIVOSO4·3H2O, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic
acid (Hepes), sodium formate, sodium acetate, sodium chloride, and
succinic acid were Sigma-Aldrich products of the highest grade available
and used without further purification. HEWL was purchased from Sigma-Aldrich
and used as received. Hempp and [VIVO(empp)2] were synthesized according to the procedure established previously.23 (link),24 (link)
Ferraro G., Paolillo M., Sciortino G., Pisanu F., Garribba E, & Merlino A. (2023). Implications of Protein Interaction in the Speciation of Potential VIVO–Pyridinone Drugs. Inorganic Chemistry, 62(21), 8407-8417.
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