Total hepatic iron content was measured in tissue homogenates (tissue homogenized in PBS containing 0.2% NP-40, 1:4 w/v tissue:buffer) processed for analyses by aliquoting into acid-cleaned polyethylene tubes, evaporating to dryness, and digesting in 16N quartz-distilled HNO3 (Optima, Fisher Scientific) at 80°C in a heat block. Following complete digestion, samples were diluted with Milli-Q water (18 Mohm/cm2) for analyses; rhodium was added as an internal standard. Iron levels were measured by high resolution inductively coupled plasma–mass spectrometry (Thermo Scientific Element XR ICP-MS), measuring masses 54Fe, 56Fe, 57Fe, and 103Rh. The analytical detection limit and measurement precision was 5.68 ng/mL 1.04% RSD, respectively.
Element xr icp ms
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Element XR ICP-MS is a high-performance inductively coupled plasma mass spectrometer (ICP-MS) designed for trace element analysis. It features a high-resolution mass analyzer that enables precise and accurate measurement of a wide range of elements in various sample types.
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Lab products found in correlation
9 protocols using element xr icp ms
1
Quantifying Total Hepatic Iron
2
Comprehensive Biomarker Assessment Protocol
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Biological samples including venous whole blood, spot urine, saliva and hair were collected from each subject upon enrollment, as described in detail in previous studies (Smith et al., 2007 (link); Eastman et al., 2013 (link); Lucas et al., 2015 (link); Butler et al., 2019 (link)). Complete overview of biomarkers can be found in Supplementary Figure 1 and Table 1 . Biological samples were processed and analyzed for metal concentrations using magnetic sector inductively coupled plasma mass spectroscopy (Thermo Element XR ICP-MS), as described elsewhere (Smith et al., 2007 (link); Eastman et al., 2013 (link); Lucas et al., 2015 (link); Butler et al., 2019 (link)).
3
Biospecimen Collection for Neurological Testing
4
Biomarker Assessment of Lead Exposure
5
Multimodal Biospecimen Collection and Analysis
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Biological samples including venous whole blood, spot urine, saliva, hair, and fingernail clippings were collected from each subject upon enrollment, as described in detail elsewhere42 (link),44 (link)–46 (link), Supplementary Material 1 , and Supplementary Table 1 . Biological samples were processed and analyzed for metal concentrations using magnetic sector inductively coupled plasma mass spectrometry (Thermo Element XR ICP-MS), as described elsewhere42 (link),44 (link)–46 (link).
6
Quantifying Silica Release Kinetics
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Particles were incubated in PBS or in ddH2O over a period of 5 weeks. The supernatant was changed and collected every second day. The Si content in all the supernatants was measured by means of inductively coupled plasma mass spectrometry (ICP-MS). ICP-MS analysis was performed by introducing extract aliquots into a mass spectrometer (Element XR ICP-MS, Thermo Scientific™, Waltham, MS, USA). Solutions were sprayed through a high-solid-type nebulizer as plasma into a thermoelectrically controlled spray chamber. The results of the analysis were then compared with a standard for identification and quantification of Si concentration. The average and standard deviation of the Si concentrations per set of samples were reported as absolute concentrations, expressed in ppm. The Si concentrations were then calculated as mg/mL. Experiments were conducted in triplicate and statistical analyses were conducted by comparing groups two by two with Student t-test.
7
Trace Element and Isotope Analysis
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For trace element and isotope analyses, sample powders were first rinsed using 1 M acetic acid to remove carbonate components from bulk mudstones. The residuals were fully digested with distilled acids of HF, HNO3, and HCl. Major and trace elements of the samples were analyzed with a Thermo Scientific Element XR ICP-MS (Inductively Coupled Plasma Mass Spectrometer) at the Yale Metal Geochemistry Center (YMGC), Yale University, and the Metal Isotope Geochemistry Lab in the Centre for Research and Education on Biological Evolution and Environment (CREBEE), Nanjing University. Lithium isotopes were analyzed with a Thermo Scientific Neptune Plus/XT MC-ICP-MS (Multicollector-Inductively Coupled Plasma Mass Spectrometer) combined with an ESI Apex-IR desolvating system at YMGC and CREBEE. Each sample, containing 50 ng of Li, was purified using AG50W-X12 (200 to 400 mesh) cation resin with 0.2 M HCl. Detailed descriptions of sample preparation, analytical methods, and accuracy follow that of previous studies (74 ) and are presented in the Supplementary Materials.
8
Comprehensive Biomarker Assessment Protocol
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Biological samples including venous whole blood, spot urine, saliva, hair, fingernails and toenails were collected from each subject upon enrollment, as described in detail in previous studies (Smith et al., 2007 (link); Eastman et al., 2013 (link); Lucas et al., 2015 (link); Butler et al., 2019 (link)). Biological samples were processed and analyzed for metal concentrations using magnetic sector inductively coupled plasma mass spectroscopy (Thermo Element XR ICP-MS), as described elsewhere (Smith et al., 2007 (link); Eastman et al., 2013 (link); Lucas et al., 2015 (link); Butler et al., 2019 (link)). A complete overview of biomarkers can be found in Table 1 , while Pearson’s correlations between biomarkers is reported in Supplementary Figure S1 . Samples with values less than the LOD were substituted with LOD/square root of 2 (Ganser and Hewett, 2010 (link)).
9
Trace Metal Analysis by ICP-MS
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