Agilent 1290 system

Manufactured by Agilent Technologies

Sourced in United States, Germany

The Agilent 1290 system is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features advanced technology to provide high-speed and high-resolution separations. The system includes components such as a solvent delivery module, an autosampler, a column compartment, and a diode-array detector.

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Lab products found in correlation

Acquity uplc ost c18 column, by Waters Corporation (3 mentions) Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (2 mentions) 6120 quadrupole msd mass spectrometer, by Agilent Technologies (2 mentions) Advance av500 spectrometer, by Bruker (2 mentions) Waters 1525ef lc system, by Waters Corporation (2 mentions) Mci gel high porous polymer, by Mitsubishi (2 mentions) Mci gel high porous polymer, by Mitsubishi Chemical Corporation (2 mentions) Ltq orbitrap xl, by Thermo Fisher Scientific (1 mentions) Api 4000 mass spectrometer, by AB Sciex (1 mentions) 6460 triple quadrupole, by Agilent Technologies (1 mentions) Masshunter quantitative analysis software, by Agilent Technologies (1 mentions) Niflumic acid, by Merck Group (1 mentions) Vincamine, by Tokyo Chemical Industry (1 mentions) Triple quad 6500, by AB Sciex (1 mentions) Agilent 1100 system, by Agilent Technologies (1 mentions) Api 4000 triple quadrupole mass spectrometer, by AB Sciex (1 mentions) Sephadex lh 20 resin, by GE Healthcare (1 mentions) Tripletof 5600 mass spectrometer, by AB Sciex (1 mentions) 2998 pda detector, by Waters Corporation (1 mentions) 6545 q tof mass spectrometer, by Agilent Technologies (1 mentions)

14 protocols using agilent 1290 system

UHPLC-MS Profiling of tRNAs

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UHPLC (Agilent 1290 system Agilent, U.S.A.) using an C 18 column (Acquity UPLC OST, 2.1×100 mm, 1.7 μm i.d., Waters, U.S.A.) at 60°C with a diode array detector. UHPLC-MS was performed using an Agilent 1290 system (Agilent Technologies, U.S.A.), equipped with a vacuum degasser, a quaternary pump, an autosampler, a diode was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
array detector and an Agilent ultrahigh definition 6545 Q-TOF mass spectrometer.
Separation was carried out on an ACQUITY UPLC OST C 18 Column (2.1×100 mm, 1.7 μm i.d., Waters, U.S.A.) at 60°C. tRNAs were separated by eluding the column at a flow rate of 0.2 mL/min with a mobile phase of 100 mM HFIP+15 mM TEAA containing MeOH of the following concentration: 1% (v:v) for 1.5 min, then 1-14% over 1.5-8.3 min, and finally 14-17 % over 8.3-16.5 min. ESI conditions were as follows: gas temperature 320°C, spray voltage 3.5 kV, sheath gas flow and temperature were set as 12 L/min and 350°C, respectively. Fractions corresponding to each chromatographic peak were collected and freeze-dried. For MS experiment, samples were analyzed in negative mode over an m/z range of 500 to 3200.

Cao K., Pan Y., Yan T., Tao P., Xiao Y., & Jiang Z. (2020). Cytotoxic effects of tRNA-derived fragments and tRNA halves from non-pathogenic Escherichia coli strain on colorectal cancer cells.

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HPLC-MS Analysis of Compounds

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HPLC was performed using an Agilent 1290 system equipped with a SunFire column (C18, 250 × 2.6 mm, Waters, USA). The mobile phase was 60% A [0.1% (v/v) aqueous acetic acid] and 40% B (acetonitrile) with a flow rate of 0.6 ml min⁻¹, column temperature 30°C and injection volume 10 μl.
The LC column was connected online to the standard ESI source of LTQ Orbitrap XL (Thermo Fisher Scientific) using a T‐port valve. The spray voltage, capillary voltage and tube lens were 4.0 kV, 16 V and 35 V respectively. The capillary temperature was 300°C with a sheath gas flow rate of 40 l min⁻¹, and an auxiliary gas flow rate of 10 l min⁻¹. External calibration of the mass spectra routinely produced a mass accuracy of better than 3 ppm. Full mass spectra were acquired in the positive ionization mode at a resolution of 30 000 with a 100–1500 Da mass range, and followed by a data‐dependent scan in the CID mode. Data acquisition and analysis were performed using XCalibur software version 4.1 (Thermo Fisher Scientific).

Chu D., Ilyas N., Peng L., Wang X., Wang D., Xu Z., Gao Q., Tan X., Zhang C., Li Y, & Yuan Y. (2021). Genomic insights on fighting bacterial wilt by a novel Bacillus amyloliquefaciens strain Cas02. Microbial Biotechnology, 15(4), 1152-1167.

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Quantitative analysis of cellular growth

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Cell growth was calculated by measuring the optical density at 600 nm (OD₆₀₀) using a spectrophotometer (Shanghai, China). Glucose and l-glutamate concentrations were determined using the SBA-40E immobilized enzyme biosensor (Shandong, China). l-proline was analyzed by reversed-phase high-pressure liquid chromatography (HPLC) using Agilent 1290 system (Agilent, Palo Alto, CA, USA). The sample preparation and the analysis procedure were carried out based on the procedure previous described (Zhang et al. 2020 (link)).

Zhang F., Liu Z.Y., Liu S., Zhang W.G., Wang B.B., Li C.L, & Xu J.Z. (2024). Rapid screening of point mutations by mismatch amplification mutation assay PCR. Applied Microbiology and Biotechnology, 108(1), 190.

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LC-MS/MS Analysis of 4A3NP Compound

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The LC-MS/MS analysis of 4A3NP was conducted using an Agilent 1290 system (Agilent Technology, Waldbronn, Germany) coupled with an API 4000 mass spectrometer (AB Sciex, Massachusetts, USA). The chromatographic column employed was a LUNA 3 μm C18 150 × 3.00 mm column (Phenomenex, CA, USA) with a guard column (SecurityGuard Cartridges RP-1, 4 × 3.0 mm, Phenomenex, CA, USA) maintained at a temperature of 40 °C. An isocratic system consisting of 40% acetonitrile in water with 0.1% formic acid (v/v) was utilized for a duration of 5 min. The injection volume was set to 10 μL, and the flow rate was maintained at 0.3 mL/min.
For multiple reaction monitoring (MRM), the positive mode was employed with the following transitions set: m/z 154.9 → 137 for 4A3NP and m/z 109.9 → 92 for 2AP (internal standard (IS)) (Table 1). The mass spectrometry system was configured with the following parameters: the IonSpray voltage (IS) set to 5000 V, the ion source temperature maintained at 350 °C, ion source gases 1 (GS1) and 2 (GS2) flowing at 50 and 55 L/h, respectively, the curtain gas (CUR) set to 20 L/h, the entrance potential (EP) set to 10 V, and the collision gas (CAD) set to 4 L/h.

Kim H.Y., Kim Y.J., Lee J.D., Kim H.R, & Seo D.W. (2024). Analytical Method Development and Dermal Absorption of 4-Amino-3-Nitrophenol (4A3NP), a Hair Dye Ingredient under the Oxidative or Non-Oxidative Condition. Toxics, 12(5), 340.

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Lipid Profiling of CDNs, Exosomes, and Cells

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Lipid standards were prepared in butanol: methanol (1:1), of which 200 µl was added to the samples of CDNs, exosomes and U937 cells. The tubes were sonicated for 30 minutes followed by centrifugation at 12000 G at 4 °C. The supernatant was analysed on Agilent 6460 triple quadrupole connected to a UHPLC Agilent 1290 system. A C18 Eclipse plus RRHD 2.1 × 50 mm analytical column with a particle size of 1.8 µm (Agilent Technologies) was used for lipid analysis. The solvents used were 40% acetonitrile in 60% water with 10 mM ammonium formate (Mobile phase A) and 10% acetonitrile in 90% isopropanol with 10 mM ammonium formate (Mobile Phase B). The peak areas of the lipids were integrated using Agilent Mass Hunter Quantitative Analysis Software. Peak areas were normalized to the internal standards.

Goh W.J., Zou S., Ong W.Y., Torta F., Alexandra A.F., Schiffelers R.M., Storm G., Wang J.W., Czarny B, & Pastorin G. (2017). Bioinspired Cell-Derived Nanovesicles versus Exosomes as Drug Delivery Systems: a Cost-Effective Alternative. Scientific Reports, 7, 14322.

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Quantitative Analysis of BK Powder

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BK powder (100 mg) was suspended in 4 mL of methanol/purified water (75 : 25, v/v). The suspension was shaken and sonicated for 5 min each. After centrifugation at 1700 g for 5 min at room temperature, the supernatant was collected as the first extract solution. Then, 4 mL of methanol/purified water (50 : 50, v/v) was added to the residue, and the second extraction solution obtained by the procedure described above was mixed with the first extract solution. The mixed extract solution undiluted or diluted 10- or 100-fold with methanol was injected into the LC-MS/MS system after pooling with a respective internal standard solution, niflumic acid (Sigma-Aldrich), or vincamine (Tokyo Chemical Industry Co.). Two systems were used for analysis of the constituents: system 1, an API4000 triple quadrupole mass spectrometer (AB SCIEX, Tokyo, Japan) equipped with an Agilent 1100 system (Agilent Technologies, Inc., Tokyo, Japan); system 2, a TripleQuad6500 (AB SCIEX) equipped with an Agilent 1290 system (Agilent Technologies, Inc.). Analytical conditions are summarized in the Supplementary Tables S1 and S2 (available online at http://dx.doi.org/10.1155/2015/853846).

Koseki J., Matsumoto T., Matsubara Y., Tsuchiya K., Mizuhara Y., Sekiguchi K., Nishimura H., Watanabe J., Kaneko A., Hattori T., Maemura K, & Kase Y. (2015). Inhibition of Rat 5α-Reductase Activity and Testosterone-Induced Sebum Synthesis in Hamster Sebocytes by an Extract of Quercus acutissima Cortex. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 853846.

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Multimodal Analytical Techniques for Compound Characterization

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Infrared radiation spectra were recorded with a Thermo Scientific Nicolet iS5 FT-IR spectrometer (Thermo, Waltham, MA, USA). Nuclear magnetic resonance spectra were measured with a Bruker Advance AV500 spectrometer (Bruker, Mannheim, Germany). LC-MS was performed with an Agilent 1290 system equipped with a 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). HRESIMS spectra were recorded with a AB Sciex TripleTOF 5600 mass spectrometer (AB Sciex, Redwood City, CA, USA). HPLC analyses were conducted on an Agilent 1260 system with a PDA detector (Agilent Technologies, USA) and a Waters 2695 system with a 2998 PDA detector (Waters, Milford, MA, USA). Preparative HPLC separation was conducted on a Waters 1525EF LC system (Waters). MCI Gel high-porous polymer (75–150 μm) was obtained from Mitsubishi Chemical Corporation (Mitsubishi, Sagamihara, Japan). Sephadex LH-20 resin (25–100 μm) was obtained from GE Healthcare Company (GE Healthcare, Danderyd, Sweden). Amberlite XAD16N resin was purchased from Taitan Company (Shanghai, China). Chemicals were purchased from Aldrich or Juyou Company (Nanjing, China).

Yang A., Hong Y., Zhou F., Zhang L., Zhu Y., Wang C., Hu Y., Yu L., Chen L, & Wang X. (2023). Endophytic Microbes from Medicinal Plants in Fenghuang Mountain as a Source of Antibiotics. Molecules, 28(17), 6301.

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UHPLC-QTOF MS Analysis of tRNAs

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Briefly, a UHPLC Agilent 1290 system (Agilent, USA) equipped with an ACQUITY UPLC OST C₁₈ column (2.1 × 100 mm internal diameter, 1.7 μm; Waters, USA) and a diode array detector. The column was maintained at 60°C and a gradient elution was performed with (A) 100 mM HFIP + 15 mM TEAA and (B) 50% MeOH in (A) as follows: 0–1.5 min, 2% (B); 1.5–8.3 min, 2%–28% (B); and 8.3–16.5 min, 28%–34% (B). The flow rate was set at 0.2 mL/min. MS data were acquired on an Agilent 6545 Q-TOF mass spectrometer with a gas temperature of 320°C, spray voltage of 3.5 kV, and sheath gas flow and temperature of 12 L/min and 350°C, respectively. Samples were analyzed in negative polarity over an m/z range of 500 to 3,200. Fractions from chromatographic peaks of individual tRNAs were collected and freeze dried.

Cao K.Y., Yan T.M., Zhang J.Z., Chan T.F., Li J., Li C., Lai-Han Leung E., Gao J., Zhang B.X, & Jiang Z.H. (2022). A tRNA-derived fragment from Chinese yew suppresses ovarian cancer growth via targeting TRPA1. Molecular Therapy. Nucleic Acids, 27, 718-732.

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Chemical Analysis and Purification Methods

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UV data were acquired on a Persee TU-1810 spectrophotometer (Persee analytics, Beijing, China). IR spectra were measured on a Thermo Scientific Nicolet iS5 FT-IR spectrometer (Thermo, United States). NMR spectra were obtained on a Bruker Advance AV500 spectrometer (Bruker, Germany). HRESIMS spectra were recorded on an Orbitrap Elite mass spectrometer (Thermo Scientific, United States). Liquid chromatography–mass spectrometry (LC-MS) was conducted with an Agilent 1290 system equipped with 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, United States). HPLC analysis was performed on a Waters 2695 system equipped with 2998 PDA detector. Total component analysis was performed on an Agilent 1290 UHPLC-6520 Q-TOF/MS. Preparative HPLC separation was performed on a Waters 1525 EF LC system (Waters Company, United States). MCI GEL high-porous polymer (75–150 μm) was purchased from Mitsubishi Chemical Corporation (Japan). Sephadex LH-20 resin (25–100 μm) was purchased from GE Healthcare Company (Sweden). XAD16N resin (20–60 mesh) was obtained from Yuanye Company (Nanjing, Jiangsu, China). Chemicals were purchased from Juyou Company or Aldrich and used without further purification unless otherwise noted.

Zhu Y., Kong Y., Hong Y., Zhang L., Li S., Hou S., Chen X., Xie T., Hu Y, & Wang X. (2022). Huoshanmycins A‒C, New Polyketide Dimers Produced by Endophytic Streptomyces sp. HS-3-L-1 From Dendrobium huoshanense. Frontiers in Chemistry, 9, 807508.

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JA Quantification in Arabidopsis Leaf

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JA levels were measured in Arabidopsis leaves in three experimental groups (WT group, cam1 group, wrky53 group). The internal standard was H₂JA [32 (link),33 (link)], and analysis was performed on an Agilent 1290 system (Agilent Technologies Co. Ltd., Palo Alto, CA, USA) with AB SCIEX-6500Qtrap (Anheuser-Busch, Saint Louis, MO, USA). Each group had three biological replicates and every replicate had more than 30 plants.

Jiao C., Li K., Zuo Y., Gong J., Guo Z, & Shen Y. (2022). CALMODULIN1 and WRKY53 Function in Plant Defense by Negatively Regulating the Jasmonic Acid Biosynthesis Pathway in Arabidopsis. International Journal of Molecular Sciences, 23(14), 7718.

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