T-tau protein, P-tau181 protein, and Aβ antibody were obtained from Abcam Ltd. (Hong Kong, China). Aβ protein, K3[Fe(CN)6]/K4[Fe(CN)6], glucose (GLU), bovine serum albumin (BSA), potassium chloride (KCl), phosphate-buffered solution (PBS, pH = 7.4, 10 mM), 2-mercaptoethylamine, and chloroauric acid (HAuCl4) were purchased from Sigma-Aldrich (Shanghai, China). The antibodies of T-tau and P-tau181 were purchased from Thermo Fisher Scientific Co., Ltd. (Beijing, China). All chemical reagents used were of high purity grade. All solution preparations were made using ultrapure water (Milli-Q, 18.2 MΩ).
K3 fe cn 6 k4 fe cn 6
Manufactured by Merck Group
Sourced in China, United States
K3[Fe(CN)6]/K4[Fe(CN)6] is an inorganic compound that serves as a laboratory reagent. It is commonly used as a redox indicator in various chemical analyses and procedures.
Automatically generated - may contain errors
Lab products found in correlation
Chloroauric acid haucl4, by Merck Group (1 mentions)
2 mercaptoethylamine, by Merck Group (1 mentions)
Aβ antibody, by Abcam (1 mentions)
P tau181 protein, by Abcam (1 mentions)
Glucose glu, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Kh2po4, by Merck Group (1 mentions)
K2hpo4, by Merck Group (1 mentions)
Ni no3 2 6h2o, by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Streptolysin o from streptococcus pyogenes, by Merck Group (1 mentions)
Dl dithiothreitol, by Merck Group (1 mentions)
Erythromycin, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
4 protocols using k3 fe cn 6 k4 fe cn 6
1
Amyloid-Tau Protein Biomarker Assay
2
Magneto-Electrochemical Immunoassay for CEA
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Distilled water was utilized in the preparation of all solutions. Dextran, K3[Fe(CN)6], K4[Fe(CN)6], K2HPO4, KH2PO4, NaOH, KCl, and Ni(NO3)2.6H2O were purchased from Sigma-Aldrich. Fe3O4 nanoparticles salts, urea, and ethanol were stocked in the laboratory. MWCNTs functionalized with carboxyl groups were purchased from US Nano. Monoclonal anti-CEA antibodies were purchased from Arigo Biolaboratories (Hsinchu, Taiwan) and CEA was purchased from Santa Cruz Biotechnology.
3
CYP3A4 Expression and Quantification
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In our study, we used human recombinant CYP3A4 (142 µM CYP3A4 in 550 mM potassium phosphate buffer (pH 7.2), containing 0.2% CHAPS, 1 mM dithiothreitol and 20% glycerol (v/v)). This enzyme was purified according to a published earlier protocol [30 (link)] in the Institute of Bioorganic Chemistry (Minsk, Belarus). The concentration of CYP3A4 was determined by a complex formation of a reduced form with carbon monoxide using the absorption coefficient ɛ450= 91 mM−1 cm−1 [31 (link)]. Acetylacetone was purchased from Fluka (Switzerland). Potassium hydroxide, potassium dihydrogen phosphate, sodium chloride were purchased from Spectrchem (Moscow, Russia). Acetic acid was purchased from Fisher Scientific (USA). Chemical reagents such as ammonium acetate, erythromycin, chloroform, didodecyldimethylammonium bromide (DDAB), disodium phosphate, DL-dithiothreitol, K3[Fe(CN)6]/K4[Fe(CN)6] and streptolysin O from Streptococcus pyogenes were purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Electrochemical Biosensor Fabrication
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Tetrachloroauric (III) acid (Sigma-Aldrich, 99%), trisodium citrate (Merck, > 99%), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Sigma-Aldrich), N-hydroxysuccinimide (NHS, Sigma-Aldrich), 4-aminothiophenol (ATP, Sigma-Aldrich), acetonitrile (Riedel, HPLC grade), ethanol (Merck, absolute), K3Fe(CN)6/K4Fe(CN)6 (Sigma-Aldrich, > 99.9%), DNA probes (Helix Bio), CTP (Orcan Company) and other chemicals of reagent grade were used as received from the supplier. All reagents and standards were prepared in ultrapure water (UPW, Human Power 1 + , Korea). The electrochemical experiments were carried out with an Ivium CompactStat (NL). The electrochemical measurements were performed using a three-electrode system under high-purity argon (99.999%) atmosphere at room temperature (24 ± 1 °C). The reference electrode was an Ag/AgCl (Sat) electrode, the auxiliary electrode a platinum wire. AuNP-modified glass fiber/carbonaceous disk electrode was used as a working electrode. The electrode surface area was adjusted as 0.071 cm2 with an O-ring in the Teflon tube.
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