Bio 5192

Manufactured by R&D Systems

BIO 5192 is a laboratory equipment product offered by R&D Systems. It is a device designed for conducting scientific research and experiments. The core function of BIO 5192 is to provide a controlled environment for various experimental procedures.

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Lab products found in correlation

Latrunculin a, by Bio-Techne (1 mentions) Y 27632 dihydrochloride, by Bio-Techne (1 mentions) Tgx 221, by MedChemExpress (1 mentions)

3 protocols using bio 5192

Blocking Antibodies for Cell Adhesion

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Blocking antibodies, isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3, 102511, BioLegend), LEAF™ purified anti-CD18 (clone M18/2, 101409, BioLegend), LEAF™ Purified Rat IgG2a, κ Isotype Ctrl (clone RTK2758, 400515, BioLegend), Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl (clone HTK888, 400969, BioLegend), BIO 5192 (R&D systems), anti-CD54 (ICAM-1; clone YN1/1.7.4; eBioscience).

Ross E.C., ten Hoeve A.L, & Barragan A. (2021). Integrin-dependent migratory switches regulate the translocation of Toxoplasma-infected dendritic cells across brain endothelial monolayers. Cellular and Molecular Life Sciences, 78(12), 5197-5212.

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Inhibition of Integrin and Cytoskeletal Dynamics

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Cells were treated with BIO 5192 (20 nM, 45 min; R&D Systems) to inhibit the VLA-4 integrin; latrunculin A (1 µM, 1 h; Tocris Bioscience) to inhibit actin polymerization; Blebb (50 µM, 10 min; MedChemExpress) to inhibit nonmuscle myosin II; Y-27632 dihydrochloride (10 µM, 1 h; Tocris Bioscience) to inhibit ROCK signaling; LY (5 µM, 1 h; MedChemExpress), BAY (Copanlisib; 64 nM, 1 h), CAL-101 (Idelalisib; 50 nM, 1 h), MLN1117 (Serabelisib; 150 nM, 1 h), and TGX-221 (85 nM, 1 h) to inhibit PI3K; and MK (5 µM, 1 h; MedChemExpress) and GSK2141795 (1 µM, 1 h) to inhibit Akt activity. Vehicle solvents were used accordingly as controls.

Hao J., Zhou H., Nemes K., Yen D., Zhao W., Bramlett C., Wang B., Lu R, & Shen K. (2021). Membrane-bound SCF and VCAM-1 synergistically regulate the morphology of hematopoietic stem cells. The Journal of Cell Biology, 220(10), e202010118.

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Evaluating GM6001 in PDAC Cells

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Reck-null PDAC cells (1.0 × 10⁵ /well) were seeded onto 6-well plates and allowed to attach overnight. Medium was then replaced with fresh medium containing GM6001 (30 μM; BIO 5192; R&D Systems) or DMSO, and the cells were incubated for 48 hours. After incubation, the cells were used for immunoblot assay, RT-qPCR, or invasion assay.

Masuda T., Fukuda A., Yamakawa G., Omatsu M., Namikawa M., Sono M., Fukunaga Y., Nagao M., Araki O., Yoshikawa T., Ogawa S., Masuo K., Goto N., Hiramatsu Y., Muta Y., Tsuda M., Maruno T., Nakanishi Y., Masui T., Hatano E., Matsuzaki T., Noda M, & Seno H. (2023). Pancreatic RECK inactivation promotes cancer formation, epithelial-mesenchymal transition, and metastasis. The Journal of Clinical Investigation, 133(18), e161847.

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