β-sitosterol was purchased from Shanghai YuanYe Biotechnology Co., Ltd. (Shanghai, China). Oil red O stain solution, filipin stain solution, dimethyl sulfoxide (DMSO), tricaine methanesulfonate (MS222), and phenylthiourea (PTU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cholesterol, egg yolk powder, and glucose were purchased from Sangon (Shanghai, China). Zebrafish feed was purchased from Shengsuo Co., Ltd. (Yantai, Shandong, China).
Cholesterol
Manufactured by Sangon
Sourced in China
Cholesterol is a versatile lab equipment used for the measurement and analysis of cholesterol levels in various biological samples. It serves as a core tool for researchers and clinicians to assess and monitor cholesterol-related health conditions.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
β sitosterol, by Yuanye Bio-Technology (1 mentions)
Tricaine methanesulfonate, by Merck Group (1 mentions)
Phenylthiourea, by Merck Group (1 mentions)
Oil red o stain solution, by Merck Group (1 mentions)
Filipin, by Merck Group (1 mentions)
Folic acid, by Sangon (1 mentions)
Sodium alginate, by Sangon (1 mentions)
Nano z, by Malvern Panalytical (1 mentions)
Talos l120c, by Thermo Fisher Scientific (1 mentions)
Whatman, by Cytiva (1 mentions)
5 protocols using cholesterol
1
Cholesterol Modulation in Zebrafish
2
Cholesterol-Loaded Alginate Nanoparticles
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Sodium alginate, folic acid, and cholesterol were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). MET, filipin, N,N'-dicyclohexylcarbodiimide (DCC), 4-(dimethyl-amino) pyridine (DMAP), (N1-(ethylimino-methylene)-N3, N3-dimethylpropane-1,3-diamine (EDC), N-hydroxysuccinimide (NHS), and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich Company (St. Louis, MO, USA). Doxorubicin, chlorpromazine hydrochloride, and EIPA were obtained from Shanghai Aladdin Bio-Chem Technology Co. Ltd. (Shanghai, China). All other chemicals and reagents used in this study were of analytical grade.
3
Liposomal Formulation Characterization
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In brief, 20 mg HSPC (A.V.T. Pharmaceutical Co. Ltd., China), 5 mg cholesterol (Sangon, China), 2 mg octadecylamine (Aladdin, China), and 1 mg CLX (Macklin, China) were dissolved into 5 mL chloroform. The mixed solution was then evaporated at 60 °C to obtain dried lipid film. Subsequently, 4 mL deionized water was added, followed by a 20‐minute sonication. Afterward, the liposome solution was extruded through 0.45- and 0.22-μm membrane filters (Millex, Ireland). The morphologies of the liposomes were observed by TEM (FEI Talos L120C, USA). The size, PDI, and zeta potential of the liposomes were analyzed by the dynamic light scattering method using a Zetasizer (Malvern Nano-ZS, UK).
4
Liposome Preparation via Thin-Film Hydration
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The thin-film hydration method plus the liposome extrusion method used in preparation was as follows: 1) At a mass ratio of 3:1:1, cholesterol-free distearoylphosphatidylcholine, distearoylphosphatidylethanolamine–polyethylene glycol 2000 (Avanti Polar Lipids, Inc., Alabaster, AL, USA), and cholesterol (Sangon Biotech Co., Ltd, Shanghai, People’s Republic of China) were added to 10 mL of chloroform (Chongqing Chuandong Chemical Group Co., Ltd, Chongqing, People’s Republic of China) and mixed thoroughly. 2) On a rotavapor (Shanghai Yarong Biochemical Instruments Factory, Shanghai, People’s Republic of China), the above-mentioned solution was rotated at 50°C at a rotational speed of 60 rpm for ~60 min. 3) After the rotation, 5 mL of NH4HCO3 (Chengdu Kelong Chemical Reagent Factory, Chengdu, People’s Republic of China) saturated solution was added, and the resulting solution was placed in an ultrasound cleaner (Ningbo Scientz Biotechnology Co., Ltd, Ningbo, People’s Republic of China) to be shaken for a few seconds. 4) The above-mentioned solution was passed through extruder filter films (Whatman Plc, Kent, UK) with apertures of 800, 400, and 200 nm, respectively. 5) The extruded solution was dissolved in a 5% glucose solution (Chengdu Kelong Chemical Reagent Factory) for dialysis.
5
Synthesis and Characterization of Novel Reagents
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Conventional experimental reagents and consumables were from Sigma Aldrich. The special reagents used in the experiment are shown below. HA, N‐[2‐(3,4‐Dihydroxyphenyl)ethyl]‐2‐methylacrylamide, and BAI were obtained from Macklin Co., Ltd. (Shanghai, China). Cholesterol, lecithin (from egg yolk), and MIA were obtained from Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). CA‐needles were obtained from Yuwell Co., Ltd. (Shanghai, China).
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