Human colorectal carcinoma cells (HT29, LS513, LS174T and HCT116) were kindly provided by Richard Camalier (Division of Cancer Treatment and Diagnosis Tumor Repository, National Cancer Institute) and Richard Hamelin (Paris, France). Colon carcinoma cell lines were maintained in DMEM (except for HCT116 cell lines, which were cultured in McCoy medium) supplemented with 5% FBS and 100 units/mL penicillin and 100 µg/mL streptomycin. The FHC cell line (established from normal human fetal colonic mucosa) was obtained from ATCC (American Type Culture Collections, VA, USA), while the hTERT and cdk4 immortalized human colon epithelial cell line (HCEC-1CT) was purchased from Evercyte. Both FHC and HCEC-1CT cell lines were maintained as described by the suppliers.
ATP (adenosine 5′-trisphosphate disodium salt hydrate), ATPγS (adenosine 5′-[γ-thio]triphosphate tetralithium salt), adenosine, QVD-Oph, Necrostatin-1 and dipyridamole (DIP) were purchased from Merck. MRS 2179 and AR-C 118925XX were purchased from Tocris, while forskolin (FSK) was obtained from abcam.
ATP (adenosine 5′-trisphosphate disodium salt hydrate), ATPγS (adenosine 5′-[γ-thio]triphosphate tetralithium salt), adenosine, QVD-Oph, Necrostatin-1 and dipyridamole (DIP) were purchased from Merck. MRS 2179 and AR-C 118925XX were purchased from Tocris, while forskolin (FSK) was obtained from abcam.