Human adipose tissue was obtained from liposuction procedures (n = 4) in compliance with the guidelines of Ethics Committee of Plastic Surgery Hospital. The lipoaspirates were washed twice, digested with 0.1% (w/v) type I collagenase (Sigma, USA) with agitation at 37°C for 30 minutes, passed through a 74 μm filter, and finally selected on the basis of adherence to plastic culture flasks. Cells were grown in MSC culture medium (Sciencell, USA) supplemented with 5% fetal bovine serum, 1% MSC growth supplement, 100 U/mL penicillin, and 100 g/mL streptomycin in a 5% CO2 incubator at 37°C, and medium was changed every 3 days. HADSCs cultured at passage 3 were used for subsequent experiments.
Msc culture medium
Manufactured by ScienCell
Sourced in United States
The MSC culture medium is a specialized cell culture medium designed to support the growth and maintenance of mesenchymal stem cells (MSCs). It provides the essential nutrients and growth factors required for the optimal in vitro expansion of MSCs.
Automatically generated - may contain errors
Lab products found in correlation
Collagenase type 1, by Merck Group (1 mentions)
Mesenchymal stem cell growth supplement, by ScienCell (1 mentions)
Mouse neutrophil isolation kit, by Miltenyi Biotec (1 mentions)
Trypsin edta 0.25, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by ScienCell (1 mentions)
Fetal bovine serum (fbs), by ScienCell (1 mentions)
2 protocols using msc culture medium
1
Isolation and Culture of Human Adipose-Derived Stem Cells
2
Isolation and Characterization of Mouse Bone Marrow-Derived Mesenchymal Stem Cells and Neutrophils
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Mouse bone marrow-derived MSCs were generally isolated from the tibia and femoral marrow compartments and were identified by flow cytometry as described previously.18 (link) The cells cultured in MSC culture medium (Science Cell, 7501) with 1% mesenchymal stem cell growth supplement (Science Cell,7552), 5% fetal bovine serum (Science Cell,0025) and 1% Penicillin/Streptomycin (Science Cell,0503). The cell incubator was maintained at 37°C with 5% CO2 and 95% humidity. Every 2–3 days the culture medium was changed and Trypsin-EDTA (0.25%) (Gibco, 25200056) was used for passaging.
Neutrophils were isolated from bone marrow of 4-week-old C57BL/6J mice. The femur and tibia were resected intactly and cut the two ends of the bone in a clean bench to flush the bone marrow out. After treatment with red cell lysis buffer, the neutrophils were isolated using Mouse neutrophil isolation kit (Miltenyi Biotec, 130–097-658) according to the manual. The isolated neutrophils were cultured in RPMI1640 medium supplemented with 10%FBS for future use.
Neutrophils were isolated from bone marrow of 4-week-old C57BL/6J mice. The femur and tibia were resected intactly and cut the two ends of the bone in a clean bench to flush the bone marrow out. After treatment with red cell lysis buffer, the neutrophils were isolated using Mouse neutrophil isolation kit (Miltenyi Biotec, 130–097-658) according to the manual. The isolated neutrophils were cultured in RPMI1640 medium supplemented with 10%FBS for future use.
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