Cells were fixed with 4% paraformaldehyde (PFA) in PBS for 10 minutes before permeabilisation in PBS containing 0.2% Triton-X for 10 minutes at room temperature. The cells were stained with DAPI (Sigma, d9542, MO, USA) to reveal DNA in cell nuclei and TRITC-phalloidin (Sigma, p1951, MO, USA) to reveal F-actin in 3% bovine serum albumin with PBS + 0.1% Tween-20. Cells were stained using anti-pMLC (S19) (Cell signaling technology #3675) or anti-pERM antibody (Cell signaling technology #3141) followed by appropriate Alexafluor-conjugated secondary antibodies (Invitrogen). PDPN-V5 was stained using anti-V5 FITC-conjugated antibody (Invitrogen R963-25). ARP2/3 was stained using Anti-p34-Arc/ARPC2 antibody (07–227 Millipore).
Anti v5 fitc conjugated antibody
Manufactured by Thermo Fisher Scientific
The Anti-V5 FITC-conjugated antibody is a fluorescein isothiocyanate (FITC)-labeled monoclonal antibody that specifically recognizes the V5 epitope tag. The antibody can be used to detect and visualize recombinant proteins that have been engineered to express the V5 tag.
Automatically generated - may contain errors
Lab products found in correlation
Anti pmlc s19, by Cell Signaling Technology (2 mentions)
Anti perm antibody, by Cell Signaling Technology (2 mentions)
Anti p34 arc arpc2 antibody, by Merck Group (2 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Phalloidin tritc, by Merck Group (2 mentions)
2 protocols using anti v5 fitc conjugated antibody
1
Cytoskeletal Protein Localization Assay
2
Cytoskeletal Protein Localization Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed with 4% paraformaldehyde (PFA) in PBS for 10 minutes before permeabilisation in PBS containing 0.2% Triton-X for 10 minutes at room temperature. The cells were stained with DAPI (Sigma, d9542, MO, USA) to reveal DNA in cell nuclei and TRITC-phalloidin (Sigma, p1951, MO, USA) to reveal F-actin in 3% bovine serum albumin with PBS + 0.1% Tween-20. Cells were stained using anti-pMLC (S19) (Cell signaling technology #3675) or anti-pERM antibody (Cell signaling technology #3141) followed by appropriate Alexafluor-conjugated secondary antibodies (Invitrogen). PDPN-V5 was stained using anti-V5 FITC-conjugated antibody (Invitrogen R963-25). ARP2/3 was stained using Anti-p34-Arc/ARPC2 antibody (07–227 Millipore).
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