Total body gamma irradiation of spontaneously hypertensive rats given sub-lethal (0.5, 3.0, and 5.0Gy) and normotensive Wistar-Kyoto rats given lethal irradiation (10.0Gy) was produced with a
Gammacell 40 Cs-137 irradiator (MDS Nordion, Mississauga, ON). The dose rate range of the irradiator was determined to be 64–68 cGy/min using Gafchromic EBT-XD dosimetry film (Bridgewater, NJ) that is placed in the middle of two high density polyethylene panels, performed annually. Four unanesthetized animals were placed in a plastic irradiation container with individual compartments at one time. Animals in the control group were sham-irradiated for the same periods of time as needed for irradiations.
Rosen E., Kryndushkin D., Aryal B., Gonzalez Y., Chehab L., Dickey J, & Rao V.A. (2020). Acute total body ionizing gamma radiation induces long-term adverse effects and immediate changes in cardiac protein oxidative carbonylation in the rat. PLoS ONE, 15(6), e0233967.