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Ab60157

Manufactured by Abcam
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Sourced in United Kingdom

Ab60157 is a mouse monoclonal antibody that recognizes the human GADPH protein. It is designed for use in Western blotting applications.

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Lab products found in correlation

Ab104224, by Abcam (1 mentions) Immu mount medium, by Thermo Fisher Scientific (1 mentions) Hoechst 33342 dye, by Thermo Fisher Scientific (1 mentions) Lsm 510 confocal microscope, by Zeiss (1 mentions) Triton x 100, by Merck Group (1 mentions) Ab39973, by Abcam (1 mentions) Ab35088, by Abcam (1 mentions) Ab107595, by Abcam (1 mentions)

3 protocols using ab60157

1

Immunocytochemistry of Hippocampal Neurons

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Primary hippocampal neuronal cultures grown on glass coverslips for 7 days were fixed using 2% paraformaldehyde and permeabilized using phosphate-buffered saline (PBS) containing 0.5% Triton X-100 (Sigma-Aldrich). The cells were then blocked using 2% bovine serum albumin in PBS containing 0.1% Triton X-100 (PBS-T) before applying the primary antibodies against NeuN (1 : 200, cat#ab104224, RRID:AB_10711040) and ALK2 (1 : 200, cat#ab60157, RRID:AB_940117, both Abcam). After overnight incubation, the cultures were washed with PBS-T and incubated for 1 h with secondary antibodies, followed by nuclear staining with Hoechst 33342 dye (Invitrogen). Coverslips were mounted using Immu-mount medium (ThermoFisher, Mississauga, Ontario, Canada) and the images were collected using an LSM 510 confocal microscope (Zeiss, Toronto, Ontario, Canada), equipped with a 10×/0.3 objective.
Beraldo F.H., Ostapchenko V.G., Xu J.Z., Di Guglielmo G.M., Fan J., Nicholls P.J., Caron M.G., Prado V.F, & Prado M.A. (2018). Mechanisms of neuroprotection against ischemic insult by stress–inducible phosphoprotein–1/prion protein complex. Journal of neurochemistry, 145(1), 68-79.
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2

Investigating BMP Signaling Pathway

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Bmp4 (ab39973; Abcam, Cambridge, UK), Bmp9 (ab35088; Abcam, Cambridge,
UK), Alk2 (ab60157; Abcam, Cambridge, UK) and Eng (ab107595; Abcam, Cambridge,
UK).
De Vilder E.Y., Cardoen S., Hosen M.J., Le Saux O., De Zaeytijd J., Leroy B.P., De Reuck J., Coucke P.J., De Paepe A., Hemelsoet D, & Vanakker O.M. (2018). Pathogenic variants in the ABCC6 gene are associated with an increased risk for ischemic stroke. Brain Pathology, 28(6), 822-831.
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3

Immunohistochemistry for ACVR1 in Tissues

Cited 1 time
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Tissues were fixed, decalcified, and then processed for paraffin embedding as previously described (6 (link), 12 (link)). Following deparaffinization, immunohistochemistry for ACVR1 was performed using a rabbit anti-ACVR1 antibody (Abcam, ab60157), as previously described (6 (link)). Hematoxylin, eosin, and Alcian blue staining was performed using standard methods.
Lees-Shepard J.B., Stoessel S.J., Chandler J.T., Bouchard K., Bento P., Apuzzo L.N., Devarakonda P.M., Hunter J.W, & Goldhamer D.J. (2022). An anti-ACVR1 antibody exacerbates heterotopic ossification by fibro-adipogenic progenitors in fibrodysplasia ossificans progressiva mice. The Journal of Clinical Investigation, 132(12), e153795.
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