The HL-60 leukemia cell volume and count were analyzed using a Z2 Coulter counter (Beckman Coulter, USA) as previously described in detail by Mazur et al. [15 (link)]. The mean cell volume and also the cell count were determined at a range 832–7346 fL, using Z2 AccuComp software (Beckman Coulter, USA).
Z2 accucomp software
Manufactured by Beckman Coulter
Sourced in United States
The Z2 Accucomp software is a tool designed to facilitate the analysis and comparison of cell populations. It provides data processing capabilities to assist in the evaluation of cell characteristics, such as size and volume, within a sample. The software enables users to perform comparative analyses between different samples or conditions.
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3 protocols using z2 accucomp software
1
Analyzing Leukemia Cell Volume and Count
2
Cell Counting and Sizing Protocol
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Cells were plated in triplicate in 35 mm dishes and grown until 80% confluent. Cells were lifted up by trypsin (Sigma, #T3924) and resuspended in pre-warmed 1 x PBS. Cell number and size was measured using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter), and processed using Z2 Accucomp software (Beckman Coulter).
3
MIA PaCa-2 Cell Size Measurement
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A total of 5 × 104 WT or SNAT7 KO MIA PaCa-2 cells, HA-SNAT7WT, HA-SNAT7N62H, or HA-SNAT721-C were seeded into 12-well plates and were cultured in low glutamine medium (0.2 mM glutamine). After 48 h, cells were lifted by 0.25% Trypsin-EDTA solution (Sigma, #5642) and then resuspended with 1× PBS. Cell size was measured by using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter) and processed by Z2 Accucomp software (Beckman Coulter).
Forty-eight hours later, the cells were harvested by trypsinization and resuspended in 1 mL PBS, diluted 1:50 with counting solution (isoton, Beckman Coulter), and cell diameters determined using a particle size counter (Coulter Z2, Beckman Coulter) with Coulter Z2 Accucomp software.
Forty-eight hours later, the cells were harvested by trypsinization and resuspended in 1 mL PBS, diluted 1:50 with counting solution (isoton, Beckman Coulter), and cell diameters determined using a particle size counter (Coulter Z2, Beckman Coulter) with Coulter Z2 Accucomp software.
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