K3 edta

Two blood samples of all the subjects were collected in the morning before the first meal: one on the day before the beginning of the procedure and the other on the day after completing the research program. Samples of whole blood (5 ml) were collected from the basilic vein into tubes containing ethylenediaminetetraacetic acid tripotassium salt (Sarstedt, S-Monovette with 1.6 mg/ml EDTA-K₃) and into tubes with a clot activator (Sarstedt, S-Monovette). The blood samples were centrifuged (10 min., 900 g 4°C), and then, the plasma and serum were immediately separated and stored at a temperature of −75°C, until biochemical analyses could be performed. The red blood cells retained from the removal of EDTA-plasma were rinsed with an isotonic salt solution, and then, 10% of the hemolysates were prepared for further analyses. Hemoglobin concentration in the hemolysates was determined by the standard cyanmethemoglobin method. The inter- and intra-assay coefficients of variations (CV) were 1.1% and 2.4%, respectively.

Blood samples of all the subjects were collected in the morning before the first meal. Samples of whole blood (5 ml) were drawn from the basilic vein of each subject and then collected into tubes containing ethylenediaminetetraacetic acid (Sarstedt, S-Monovette with 1.6 mg/ml EDTA-K₃) and into tubes with a clot activator (Sarstedt, S-Monovette). The blood samples were centrifuged (10 min, 900g at 4°C), and then the plasma and serum were immediately separated and stored at the temperature of −75°C, until biochemical analyses could be performed. In turn, the red blood cells retained from the removal of EDTA plasma were rinsed with isotonic salt solution and then 10% of the hemolysates were prepared for further analyses. The hemoglobin concentration in the hemolysates was determined by the standard cyanmethemoglobin method. The inter- and intra-assay coefficients of variations (CV) were 1.1% and 2.4%, respectively.

Blood samples of all the subjects were collected in the morning before the first meal one day before the beginning and one day after the completion of the research program. Samples of whole blood (5 ml) were drawn from the basilic vein and then collected into tubes containing ethylenediaminetetraacetic acid (Sarstedt, S-Monovette with 1.6 mg/ml EDTA-K₃) and into tubes with a clot activator (Sarstedt, S-Monovette). The blood samples were centrifuged (10 min., 900g 4°C) and then the plasma and serum were immediately separated and stored at the temperature of −75°C, until biochemical analyses could be performed. The red blood cells retained from the removal of EDTA plasma were rinsed with isotonic salt solution, and then 10% of the hemolysates were prepared for further analyses. The hemoglobin concentration in the hemolysates was determined by the standard cyanmethemoglobin method. The inter- and intra-assay coefficients of variations (CV) were 1.1% and 2.4%, respectively.

Blood samples (10 mL) were collected from the ulnar vein, in the morning, before breakfast. Blood was collected in standard blood tubes with EDTA (1.6 mg/mL EDTA- K3; S-Monovette, SARSTEDT). The samples for serum analysis were centrifuged at 4000× g rpm for 10 min at 4 °C and stored at −80 °C. Plasma and serum samples were subsequently frozen and stored at −80 °C until biochemical analyses could be performed.

Blood samples of all the subjects were collected in the morning before the first meal. Samples of whole blood (5 ml) were collected from the basilic vein into tubes containing ethylenediaminetetraacetic acid tripotassium salt (Sarstedt, S-Monovette with 1.6 mg/ml EDTA-K3) and into tubes with a clot activator (Sarstedt, S-Monovette). Total cholesterol, HDL cholesterol, and LDL cholesterol (T-Chol, HDL-Chol, and LDL-Chol) and triglyceride (TG) concentrations in serum were estimated using routine techniques (COBAS INTEGRA 400 plus analyzer, Roche Diagnostics, Mannheim, Germany). Concentrations were expressed in mg/dl.
Estimated GFR (eGFR) was calculated using Cockroft-Gault formula. Fasting glucose and hsCRP levels were also determined in all patients.

Blood samples of all the subjects were collected in the morning before the first meal. Samples of whole blood (5 ml) were drawn from a basilic vein of each subject and then collected into tubes containing ethylenediaminetetraacetic acid (Sarstedt, S-monovette with 1.6 mg/ml EDTA-K_3; catalogue number 04.1931) and into tubes with a clot activator (Sarstedt, S-monovette, catalogue number 04.1934). The blood samples were centrifuged (10 min., 900g, 4°C), and then the plasma and serum were immediately separated and stored at the temperature of −70°C, until biochemical analyses were performed. In turn, the red blood cells retained from removal of EDTA-plasma were rinsed with isotonic salt solution and then 10% of hemolysates were prepared for further analyses. Hemoglobin concentration in hemolysates was determined by standard cyanmethemoglobin method. The inter- and intra-assay coefficients of variations (CV) were, respectively, 1.1% and 2.4%.