Rayonet photochemical chamber reactor rpr 200

All reagents and deuterated solvents used for reactions and spiking experiments were purchased from Sigma-Aldrich and were used without further purification. All photochemical reactions were carried out in Norell Suprasil quartz NMR tubes purchased from Sigma-Aldrich using Hg lamps with principal emission at 254 nm in a Rayonet photochemical chamber reactor RPR-200, acquired from The Southern New England Ultraviolet Company. StarLab is an in-house constructed photoreactor that delivers broadband UV-Vis (from 220 nm to ~750 nm by using water as an optical filter) irradiation to a sample from a 75W xenon lamp manufactured by Horiba³⁸ . A Mettler Toledo SevenEasy pH Meter S20 was used to monitor the pH, and degassed H₂O or D₂O was achieved by four rounds of freeze-pump-thaw cycling. ¹H-, and ¹³C-nuclear magnetic resonance (NMR) spectra were acquired using a Bruker Ultrashield 400 Plus or Bruker Ascend 400 operating at 400.1, and 100.6 MHz, respectively. Samples consisting of H₂O/D₂O mixtures were analyzed using HOD suppression to collect ¹H-NMR data. Chemical shifts (δ) are shown in ppm. Coupling constants (J) are given in Hertz and the notations s, d, t represent the multiplicities singlet, doublet, and triplet. The conversion yields were determined by relative integrations of the signals using a known amount of acetamide as internal reference in the ¹H-NMR spectrum.