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Axiocam hrc microscope

Manufactured by Zeiss
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Sourced in Germany

The AxioCam HRc is a high-resolution digital microscope camera from Zeiss. It features a 12.6-megapixel CCD sensor and can capture images with a resolution of up to 4080 x 3072 pixels. The camera is designed to be integrated with Zeiss microscopes and provides reliable image capture for a variety of microscopy applications.

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Lab products found in correlation

Axiovision rel software, by Zeiss (2 mentions) Cytoseal, by Thermo Fisher Scientific (2 mentions) Biotinylated conjugated donkey anti rabbit antiserum, by Jackson ImmunoResearch (2 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions) Axiovision 4, by Zeiss (1 mentions) Axiovert 200, by Zeiss (1 mentions) Ab150079, by Abcam (1 mentions)

Close spelling variants of this product

AxioCam HRc microscope camera AxioCam HRc digital microscope camera AxioCam HRc color microscope camera AxioCam HRc AxioCam microscope AxioCam

7 protocols using axiocam hrc microscope

1

Quantifying Autophagy in PC3 Cells

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PC3 cells were seeded in 24-well plates at a density of 8 × 104 cells/well and transfected with a GFP-tagged LC3 plasmid (GFP-LC3; Addgene plasmid # 11546)52 (link) using FuGENE® HD Transfection Reagent (Roche). Twenty hours after transfection, PC3 cells were treated as indicated and the formation of GFP-LC3 puncta in response to chemicals was observed for 48 hours thereafter using the Zeiss Axiovert 200 inverted fluorescence microscope equipped with the AxioCam HRc microscope color camera and the AxioVision 4.8 software (all from Carl Zeiss, Göttingen, DE).
Modernelli A., Naponelli V., Giovanna Troglio M., Bonacini M., Ramazzina I., Bettuzzi S, & Rizzi F. (2015). EGCG antagonizes Bortezomib cytotoxicity in prostate cancer cells by an autophagic mechanism. Scientific Reports, 5, 15270.
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2

Tracing PVHCrh Neuronal Projections

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To track PVHCrh projections in the brain, we stereotaxically microinjected 40 nl of AAV8-FLEX-hsyn-ChR2-mCherry (UNC Vector Core) into the unilateral PVH (from bregma: A/P 0.26, M/L 0.76, D/V −4.8) of adult Crh-ires-Cre mice44 (link) (a gift from Dr. Bradford Lowell) using a glass micropipette and an air pressure injection system. Mice were perfused 7 weeks later, the brains were removed and stored in 10% formalin overnight followed by transfer into 20% sucrose. Sectioned brain tissue (30um) was quenched in 0.3% H2O2 for 30 minutes, and then incubated overnight with rabbit polyclonal antiserum against mCherry (1:5000; Clontech #632496). Between this incubation and each subsequent incubation, tissue was washed with 1XPBS for 5 min × 5 times. On the second day, sections were incubated with biotinylated-conjugated donkey anti-rabbit antiserum (1:500, Jackson#712065152) for 2 h, followed by avidin-biotin complex (ABC) solution for 1h. Sections were incubated in DAB (3, 3′-Diaminobenzidine), TBS, H2O2 for less than 5 minutes. Sections were mounted and coverslipped (Cytoseal, Thermoscientific). Images were captured with a Zeiss AxioCam HRc microscope and AxioVision Rel software (v4.8).
Zhang R., Asai M., Mahoney C.E., Joachim M., Shen Y., Gunner G, & Majzoub J.A. (2016). Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice. Molecular psychiatry, 22(5), 733-744.
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3

Femoral Head Histological Analysis

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The femoral heads obtained from rats were fixed in 4% paraformaldehyde for 48 h and decalcified by using 10% diamine ethylene tetraacetic acid (EDTA, Sigma) for 4 weeks. Then, the femoral head samples were embedded in paraffin, sectioned into 7-µm-thick slices, and placed on slides. Finally, the femoral head slices were stained using H&E and Masson sealed with neutral resins. We observed the results under an AxioCam HRC microscope (Carl Zeiss, Oberkochen, Germany).
Li W., Li W., Zhang W., Wang H., Yu L., Yang P., Qin Y., Gan M., Yang X., Huang L., Hao Y, & Geng D. (2023). Exogenous melatonin ameliorates steroid-induced osteonecrosis of the femoral head by modulating ferroptosis through GDF15-mediated signaling. Stem Cell Research & Therapy, 14, 171.
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4

Tracing PVHCrh Neuronal Projections

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
To track PVHCrh projections in the brain, we stereotaxically microinjected 40 nl of AAV8-FLEX-hsyn-ChR2-mCherry (UNC Vector Core) into the unilateral PVH (from bregma: A/P 0.26, M/L 0.76, D/V −4.8) of adult Crh-ires-Cre mice44 (link) (a gift from Dr. Bradford Lowell) using a glass micropipette and an air pressure injection system. Mice were perfused 7 weeks later, the brains were removed and stored in 10% formalin overnight followed by transfer into 20% sucrose. Sectioned brain tissue (30um) was quenched in 0.3% H2O2 for 30 minutes, and then incubated overnight with rabbit polyclonal antiserum against mCherry (1:5000; Clontech #632496). Between this incubation and each subsequent incubation, tissue was washed with 1XPBS for 5 min × 5 times. On the second day, sections were incubated with biotinylated-conjugated donkey anti-rabbit antiserum (1:500, Jackson#712065152) for 2 h, followed by avidin-biotin complex (ABC) solution for 1h. Sections were incubated in DAB (3, 3′-Diaminobenzidine), TBS, H2O2 for less than 5 minutes. Sections were mounted and coverslipped (Cytoseal, Thermoscientific). Images were captured with a Zeiss AxioCam HRc microscope and AxioVision Rel software (v4.8).
Zhang R., Asai M., Mahoney C.E., Joachim M., Shen Y., Gunner G, & Majzoub J.A. (2016). Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice. Molecular psychiatry, 22(5), 733-744.
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5

Histological Analysis of Rat Femoral Heads

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The femoral heads of rats were immersed in 4% paraformaldehyde for 48 h. After 4 weeks of decalcification in 10% ethylenediaminetetraacetic acid, the specimens were dehydrated, paraffin embedded, sliced (6 μm), and mounted onto glass slides. After hematoxylin and eosin (H&E) staining, the sections were mounted with neutral resins and observed under an AxioCam HRC microscope (Carl Zeiss, Oberkochen, Germany).
Yang N., Sun H., Xue Y., Zhang W., Wang H., Tao H., Liang X., Li M., Xu Y., Chen L., Zhang L., Huang L, & Geng D. (2021). Inhibition of MAGL activates the Keap1/Nrf2 pathway to attenuate glucocorticoid‐induced osteonecrosis of the femoral head. Clinical and Translational Medicine, 11(6), e447.
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6

Immunofluorescence Analysis of Becn1 Expression

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We used immunofluorescence (IF) staining to detect the expression of Ferr-DEGs between the two groups. Antigen repair of the sections was performed, and the sections were blocked with horse serum. Finally, the primary antibody was incubated at 4°C overnight, and fluorescent-labeled secondary antibodies (ab150079, abcam, UK) were incubated at room temperature for 1 hour. The localization and protein expression level of Becn1 (A7353, ABclonal, China) were observed by IF staining under an AxioCam HRC microscope (Carl Zeiss, Germany).
Xia Y., Zhang H., Wang H., Wang Q., Zhu P., Gu Y., Yang H, & Geng D. (2022). Identification and validation of ferroptosis key genes in bone mesenchymal stromal cells of primary osteoporosis based on bioinformatics analysis. Frontiers in Endocrinology, 13, 980867.
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7

Multimodal Histopathological Analysis of Tissue Remodeling

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All the calvaria were fixed in 10% formalin for a minimum of 2 days. After assessment by high-resolution micro-CT, the calvaria were decalcified with 10% ethylenediaminetetraacetic acid (EDTA, Sigma) for 28 days, embedded and sectioned into 6 μm sections. Lung, liver and kidney tissues from the four groups were harvested immediately after the mice were sacrificed and then fixed in 10% formalin. Next, they were embedded and sliced into 6 μm sections. H&E staining was performed to investigate the morphological changes of tissues following the manufacturer’s protocols.
A Masson trichrome staining kit (Leagene Biotech. Co., Ltd, Beijing) was used following the manufacturer’s protocols to evaluate the osteogenesis of new immature collagen by detecting the blue area after coloration of the tissues. The new immature collagen in the blue zone implied new bone formation. Morphological analysis of new immature collagen was performed through quantifying the blue staining average area percentage (average%) of five fields in each histopathologic section. The pictures were captured through Axio-Cam HRC microscope (Carl Zeiss, Germany).
Wang Q., Zhang W., Peng X., Tao Y., Gu Y., Li W., Liang X., Wang L., Wu Z., Wang T., Zhang H., Liu X., Xu Y., Liu Y., Zhou J, & Geng D. (2022). GSK-3β suppression upregulates Gli1 to alleviate osteogenesis inhibition in titanium nanoparticle-induced osteolysis. Journal of Nanobiotechnology, 20, 148.
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