Ab17677

Manufactured by Abcam

Sourced in China

Ab17677 is a primary antibody that recognizes the Histone H3 (tri methyl K4) protein. This antibody is intended for use in immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab18208, by Abcam (1 mentions) Ab24834, by Abcam (1 mentions) Ab31830, by Abcam (1 mentions) Anti h3, by Active Motif (1 mentions) Anti h2a, by Merck Group (1 mentions) Hmgn2, by Cell Signaling Technology (1 mentions) Atto 488 goat anti mouse igg, by Merck Group (1 mentions) Goat anti rabbit igg atto 488, by Merck Group (1 mentions) Ab1791, by Abcam (1 mentions) Dynabead, by Thermo Fisher Scientific (1 mentions) C1158, by Beyotime (1 mentions)

4 protocols using ab17677

Antibody Immunostaining Protocol

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The following primary rabbit antibodies were purchased from Abcam: anti-H1.2, ab17677; H1.5, ab18208; HMGB2, ab124670; Ki67, ab15580; H3S10p, ab5176; CTCF, ab128873; SMC2, ab10412; RAD21, ab154769; H1x, ab31972. From Cell Signaling: anti-HMGN1, #5692; HMGN2, #9437. From Sigma-Aldrich: anti-H1.4, H7665. From Millipore: anti-H2A, #07-146. The following mouse antibodies were purchased from Abcam: anti-H3, ab24834; H4, ab31830; H2B, ab52584. From Active Motif: anti-H3, #61475. mAb PL2-6 was a gift from M. Monestier (Temple Univ.), see previous use [26,27]. Secondary antibodies included Invitrogen Alexa 568 goat anti-rabbit IgG, Sigma-Aldrich Atto 488 goat anti-rabbit IgG and Atto 488 goat anti-mouse IgG.

Olins A.L., Gould T.J., Boyd L., Sarg B, & Olins D.E. (2020). Hyperosmotic stress: in situ chromatin phase separation. Nucleus, 11(1), 1-18.

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Cytoplasmic Protein Isolation and Immunoprecipitation

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Cytoplasmic fractions were isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents as described above, then incubated overnight with Dynabeads® (Invitrogen) coated with rabbit IgG, antibodies to αS (NACP98, Mayo Clinic [7 (link)]), H1.2 (ab17677, Abcam), H3 (ab1791, Abcam), following the manufacturer’s instruction. Proteins bound to the beads were released by boiling in sample loading buffer and probed with antibodies of interest by western blotting.

Jiang P., Gan M., Yen S.H., McLean P.J, & Dickson D.W. (2016). Histones facilitate α-synuclein aggregation during neuronal apoptosis. Acta neuropathologica, 133(4), 547-558.

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Antibody-based Protein Expression Analysis

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The antibodies against ILK (sc-13075), Bcl-2 (sc-492), Bax (sc-493), beta-actin (sc-1616), GAPDH (sc-25778), NF-κB p65 (sc-372), IKB alpha (IKBa; sc-371), and IKBa (ser32; sc-7977) were purchased from Santa Cruz Biotechnology Inc., CA, USA. The antibodies against Histone 1.2 (ab17677) and NF-κB p65 (phospho-S536; ab86299) were purchased from Abcam (Shanghai, China). Kits for measuring caspase-3 (C1116) and caspase-9 (C1158) activities and a lactate dehydrogenase (LDH) assay kit (C0017) were obtained from Beyotime Institute of Biotechnology (Haimen, China). All other reagents were obtained from common commercial sources.

Liu Q., Tan W., Che J., Yuan D., Zhang L., Sun Y., Yue X., Xiao L, & Jin Y. (2018). 12-HETE facilitates cell survival by activating the integrin-linked kinase/NF-κB pathway in ovarian cancer. Cancer Management and Research, 10, 5825-5838.

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Stability Assessment of H1.2 KxUb Conjugates

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To determine the stability of the H1.2 KxUb conjugates in human cell lysates, 4.7 µM H1.2 or H1.2 KxUb were incubated in HEK 293T cell lysates (5 mg/ml). Samples were incubated at 4 °C, 25 °C, or 37 °C for up to 24 h and finally analyzed by western blot with anti-H1.2 (Abcam ab17677) in dilution 1:1000 and anti-Ub (BioLegend P4G7), dilution 1:1000, antibodies. For unprocessed scans see also the Source Data file.

Höllmüller E., Geigges S., Niedermeier M.L., Kammer K.M., Kienle S.M., Rösner D., Scheffner M., Marx A, & Stengel F. (2021). Site-specific ubiquitylation acts as a regulator of linker histone H1. Nature Communications, 12, 3497.

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