Human umbilical vein endothelial cells (HUVEC) and HEK293 cells were maintained as previously described [28 (link)]. Fibroblast, MDC1 and HeLa cells were grown in DMEM media according to standard protocols [39 (link)]. Primary renal Proximal Tubule Epithelial cells (PTEC) were obtained from ATCC and maintained according to the manufacturer's protocol. Osteosarcoma SAOS2 (Lonza), KHOS (Lonza) and malignant glioma [U251and M049 (gift from Dr. R. Godbout)] were cultured using McCoy's 5A (for SAOS2) and DMEM (KHOS and glioma) media according to the Lonza protocol.
Saos2
Manufactured by Lonza
Sourced in Switzerland
The SAOS2 is a cell line derived from human osteosarcoma. It is a type of lab equipment used for biological research and experiments. The SAOS2 cell line is widely used in the study of bone and cancer biology.
Automatically generated - may contain errors
Lab products found in correlation
Mg 63, by Lonza (2 mentions)
Rpmi 1640, by Lonza (2 mentions)
Saos 2, by American Type Culture Collection (2 mentions)
Penicillin streptomycin, by InvivoGen (1 mentions)
Mccoy 5a medium, by Lonza (1 mentions)
Geneticin g418, by InvivoGen (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Mg 63, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
Niclosamide, by Cayman Chemical (1 mentions)
Pyrvinium pamoate, by Merck Group (1 mentions)
Sirna duplexes, by Santa Cruz Biotechnology (1 mentions)
Mp0040, by Merck Group (1 mentions)
Saos2, by Korean Cell Line Bank (1 mentions)
Dulbecco modified eagle medium (dmem), by Lonza (1 mentions)
Rpmi 1640 medium, by Lonza (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
Sjsa 1, by American Type Culture Collection (1 mentions)
Crl1554, by American Type Culture Collection (1 mentions)
4 protocols using saos2
1
Culturing Diverse Cell Lines
2
Osteosarcoma Cell Line Culture Protocols
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OS cell lines (U2-OS (HTB-06), Saos-2 (HTB-85), MG-63 (CRL-1427)) were of human origin (ATCC, Manassas, VA, USA). U2-OS and MG-63 cells were cultured in Eagle’s Alpha-Minimal Essential medium (EMEM) supplemented with 2.5 mM (w/v) L-glutamine, 10% (v/v) FCS, and 1% (v/v) penicillin/streptomycin (Gibco). Saos-2 cells were cultured in McCoy 5A medium (Lonza, Switzerland) supplemented with 2.5 mM (w/v) L-glutamine, 10% (v/v) FCS, and 1% (v/v) penicillin/streptomycin. OS cell lines were cultivated under standardized conditions (5% CO2, 37 °C, 98% humidity) and medium was exchanged twice per week. Human osteoblasts (hFOB1.19), provided by the Core Facility Alternative Biomodels & Preclinical Imaging of the Medical University Graz, were cultivated at 34 °C in a 1:1 (v/v) mixture of Ham’s F12 medium and Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 2.5 mM (w/v) L-glutamine, 10% (v/v) FCS, 1% (v/v) penicillin/streptomycin, and 0.3 mg/mL G-418 (Geneticin, InvivoGen, Toulouse, France). All cell lines were split at 80–90% confluence and cell numbers were determined by CASY (OMNI Life Sciences, Bremen, Germany). The mycoplasma test was performed every three months.
3
Osteosarcoma Cell Lines and Reagents
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Human osteosarcoma cell lines (MG-63 and SAOS-2) were obtained from the Korean Cell Line Bank (Seoul, Korea). MG-63 and SAOS-2 cells were cultured in RPMI 1640 (Lonza, Basel, Switzerland) with 10% FBS maintained at 37 °C in a humidified atmosphere containing 5% carbon dioxide. The human fetal osteoblastic hFOB 1.19 cell line was cultured according to established ATCC protocols. Mycoplasma infection was tested regularly using a PCR-based kit (MP0040; Sigma, St. Louis, MO, USA). siRNA duplexes directed against human Axin2 (sc-35087) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Niclosamide (2′,5-dichloro-4′-nitrosalicylanilide) was purchased from Cayman (Ann Arbor, MI, USA), and pyrvinium (6-(dimethylamino)-2-[2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl) ethenyl]-1-methyl-4,4′-methylenebis[3-hydroxy-2-naphthalenecarboxylate] (2:1)-quinolinium) was purchased from Sigma-Aldrich (Burlington, MA, USA). Niclosamide (Cayman, Ann Arbor, MI, USA) and pyrvinium pamoate (Sigma, St. Louis, MO, USA) were solubilized in DMSO for in vitro experiments.
4
Murine and Human Osteosarcoma Cell Lines
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The murine osteosarcoma MOS-J cell line, called MOS-J/Native in this study, derived from a spontaneous C57BL/6 mouse osteosarcoma, was provided by Prof. L. Shultz [21 ]. Two subclones derived from this cell line were also used in the experiments [6 (link)]. The first clone, MOS-J/PG1, revealed a high proliferation rate in vitro in contrast to the second, MOS-J/A3N. These clones, as well as the MOS-J/Native, were grown in RPMI1640 medium (Lonza, Walkersville, MD, USA) supplemented with 5% fetal bovine serum (FBS; Hyclone, Logan, UT, USA) and a mix of 100 U/mL of penicillin and 100 µg/mL of streptomycin (Lonza). The human osteosarcoma cell lines G292 (clone A141B1), KHOS/NP (R-970-5) named HOS in the manuscript, 143B, MG63, SJSA-1, and SaOS2 were purchased at the American Type Culture Collection (ATCC, Manassas, VA, USA), respectively, under references CRL-1423, CRL-1554, CRL-8303, CRL-1427, CRL-2098, and HTB-85. The CAL-72 cell line was purchased at the DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ, Leibniz Institute, Braunschweig, Germany) under reference ACC-439. G292, HOS, 143B, MG63, SaOS2, and CAL-72 cell lines were cultured in DMEM (Lonza) and the SJSA-1 cell line in RPMI-1640 (Lonza), both supplemented with 10% FBS.
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