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9 protocols using sodium bisulfite

1

Antimicrobial Cassava Starch Biopolymer

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Cassava starch and octenyl succinate cassava starch ester are self-made in the laboratory; Chitosan (degree of deacetylation ≥ 90%) comes from Hefei Bomei Biotechnology Co., Ltd; Octenyl succinic anhydride comes from Guangzhou Guangjia Chemical Co., Ltd; Zinc oxide (particle size 30 nm) comes from cool chemistry; Sodium hydroxide, silver nitrate, glycerol, petroleum ether and absolute ethanol all come from Xilong science; 3,5-Dinitrosalicylic acid comes from Tianjin Dalong Chemical Reagent Factory; ε- Polylysine, hydrochloric acid, phenolphthalein, crystalline phenol, sodium bisulfite, potassium sodium tartrate, LB medium, agar and methyl orange were all purchased from Sinopharm Chemical Reagent Co., Ltd. PBS buffer solution (pH = 6.8) was purchased from Lvyuan Biotechnology Co., Ltd. the reagents were all analytical pure. Mouse embryonic fibroblasts) are from Wuhan prosai biotechnology company; Fetal bovine serum was purchased from Hangzhou Sijiqing; DMEM culture medium was purchased from Beijing solebao; Cell counting kit-8 was purchased from Shanghai Yisheng; E.coli and S.aureus come from Guangdong huankai Biotechnology Co., Ltd.
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2

Synthesis of Polymer Materials

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Acrylamide, sodium bisulfite, ammonium persulfate, sodium hydroxide, and N, N’-methylene bisAcrylamide are chemically pure reagents that were purchased from Sinopharm Chemical Reagent Co. Ltd., China. Nitrogen, >99% purity; 10# white oil; 4A zeolite powder; lithium soap clay; sericite; and industrial products were purchased from Shandong Usolf Chemical Technology Co., Ltd., China.
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3

Oilfield Chemical Reagent Synthesis

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St, BA, sodium hydroxide, potassium persulfate and sodium bisulfite were purchased from Sinopharm Chemical Reagent Co., Ltd. AMPS was purchased from Jiangxi Changjiu Agricultural Chemical Co., Ltd. Class G oil well cement was purchased from Sichuan Jiahua Cement Co., Ltd. The dispersant SGJZ was supplied by Shengli Oilfield.
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4

Synthesis and Characterization of Hydrogels

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Monomeric acrylamide (AM), metharoycholine (DMC), and itaconic acid (IA) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China), Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), and Tianjin Chemical Reagent Co., Ltd. (Tianjin, China), respectively. Microcrystalline cellulose (MCC) and sodium bisulfite were purchased from Sinopharm Chemical Reagent Co., Ltd., too. Polyethyleneimine (PEI, with Mw ~ 750,000, 50 wt.% in H2O) was purchased from Sigma-Aldrich Co. LLC (Shanghai, China). The rest of the chemicals were ordered form Nanjing Chemical Reagent Co., Ltd. (Nanjing, China). All chemicals are analytical grade and used without further purification. QCM-D sensors were AT cut quartz crystals and were supplied by Biolin Scientific Co., Ltd. (Gothenburg, Sweden) with silica surface.
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5

Synthesis of Diquat-Functionalized Silica Particles

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Sodium sulfite (Na2SO3, 97%), sodium bisulfite (NaHSO3, 99%), and cetyltrimethylammonium bromide (CTAB, 99%) were obtained from Sinopharm Chemical Reagent Co, Ltd. (Beijing, China). Diquat dibromide (DQ, 41.5%) was purchased from Shandong LvFeng Pesticide Co. Ltd. (Qingzhou, Shandong province, China). 20% DQ aqueous solution was obtained from Shandong Luba Chemical Co., Ltd. (Jinan, China). Tetraethyl orthosilicate (TEOS) with the purity of 99% was obtained from Fluorochem Ltd. (Hadfield, UK). 3-Glycidoxypropyltrimethoxysilane (GPTMS, 97%) was purchased from J&K Scientific Ltd. (Beijing, China). All other chemicals were commercially available and used as received. Deionized water used for reactions and the treatment process were attained from a Milli-Q water system (Millipore Corporation, Bedford, MA, USA).
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6

Synthesis of Sodium Hydrosulfide from Sodium Nitrite and Sodium Bisulfite

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Sodium nitrite (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) aqueous solution was added to a constant-pressure funnel at a molar ratio of 4.25:1, and the Sodium nitrite solution was added to a three-mouth flask dropwise to react with sodium bisulfite (Sinopharm Chemical Reagent Co., Ltd.) at 90°C. After a 90-minute isothermal reaction, the esterification agent sodium trisulfonate (N(SO3Na)3) was prepared.29 The pH was adjusted to 7 with 1 mol/L of sodium hydroxide solution at 40°C; then, SH (Liyang Biomaterials Co., China) was added (nNaNO2/mSH = 6/403 mol/g), and the mixture was magnetically agitated for 6 hours. The solution was washed with anhydrous ethanol and then dialyzed for 72 hours. Finally, SHS was obtained by isothermal drying. The reaction steps are shown in Figure 1.
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7

Synthesis and Characterization of Cationic Polymers

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Acrylamide (AM, CP), methacryloyloxyethyl trimethyl ammonium chloride (DMC, 75%), ammonium persulfate (APS, AR), sodium bisulfite (SBS, AR), hydrochloric acid (HCl, AR), calcium chloride (CaCl2, AR), calcium carbonate (CaCO3, AR), and anhydrous ethanol (>99.7%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), 2,2′-Azobis(2-methylpropionitrile) (AIBN,99%), 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (V-50,97%), and pyrene (97%) were purchased from Aladdin Reagent Network (Shanghai, China) alongside octadecyl polyoxyethylene methacrylate (OEMA, 50%) were purchased from Shanghai Palimo New Materials Co., Ltd.(Shanghai, China).
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8

Removal of Organic Pollutants using Reagents

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SMX, tert-butyl alcohol (TBA) and fulvic acid were produced by Aladdin Reagent Company, China. Ferric sulfate and sodium bisulfite were obtained from Sinopharm Chemical Reagent Corporation Ltd., China. Methanol and isopropanol (IPA) were the products of Fisher corporation. H2SO4, NaOH, NaCl, NaNO3, NaHCO3, Na2SO4, Na2S2O3, CuSO4, Mg(NO3)2, Ca(NO3)2 were all of analytical grade and purchased from Chengdu Kelong Chemical Reagent Co., Ltd, China. Deionized water was used in all experiments.
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9

Acrylamide-Based Hydrogel Synthesis

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Acrylamide (AM) and ammonium peroxydisulfate were provided by Beijing J&K Scientific Co., Ltd. (Beijing, China). Sodium bisulfite, sodium chloride, calcium chloride, magnesium chloride, and sodium dodecyl benzene sulfonate were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). N, N’-methylene diacrylamide (MBA) and sericite (3000 mesh) were provided by Shanghai Mindray Chemical Technology Co., Ltd. (Shanghai, China). The main chemicals and reagents used were of analytical grade.
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