AS-IV (HPLC ≥ 98.81%) and fine particulate matter standards were respectively purchased from Chengdu Munster Company (China) and National Institute of Standards and Technology (USA). 3-Methyladenine (3-MA) was purchased from Selleck, and polyvinylidene fluoride membranes were purchased from Bio-Rad (USA). Antibodies against the following targets were obtained from Abcam (Cambridge, UK): LC3B (ab48394), p62 (ab56416), PI3K (ab182651), Akt (ab185633), p-Akt (ab38449), mTOR (ab2732), p-mTOR (ab137133), Lamin B (ab16048), and GAPDH (ab181602). An antibody against p-PI3K (AF3242) was obtained from Affinity Biosciences (USA). An antibody against p65 (8242S) was obtained from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary antibody (GB23303) and 4°C tissue radioimmunoprecipitation assay lysates were obtained from Servicebio (Wuhan, China).
Ab185633
Manufactured by Abcam
Sourced in United Kingdom
Anti-Histone H3 (phospho S10) antibody (Ab185633) is a rabbit polyclonal antibody that recognizes histone H3 phosphorylated at serine 10. This antibody is suitable for use in various applications including Western blot, immunocytochemistry, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Ab38449, by Abcam (2 mentions)
Ab182651, by Abcam (1 mentions)
Ab181602, by Abcam (1 mentions)
Ab2732, by Abcam (1 mentions)
Ab137133, by Abcam (1 mentions)
Ab56416, by Abcam (1 mentions)
Af3242, by Affinity Biosciences (1 mentions)
3 methyladenine 3 ma, by Selleck Chemicals (1 mentions)
Ab16048, by Abcam (1 mentions)
Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions)
Ab151549, by Abcam (1 mentions)
Ab181603, by Abcam (1 mentions)
Ab75732, by Abcam (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
Ab109199, by Abcam (1 mentions)
Ab131443, by Abcam (1 mentions)
Ab4051, by Abcam (1 mentions)
Ab28472, by Abcam (1 mentions)
Ab131442, by Abcam (1 mentions)
Ab49822, by Abcam (1 mentions)
3 protocols using ab185633
1
Autophagy Pathway Regulation Assay
2
Western Blot Analysis of LGR5, PI3K, AKT
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Western blot analysis was performed according to standard western blot procedures. First, the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 10% acrylamide gels. Following, the protein bands were transferred onto nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After being blocked using 5% nonfat milk, the membranes were incubated with the following primary antibodies from Abcam, Cambridge, UK: rabbit anti-LGR5 polyclonal antibodies (ab75732; 1:1,000), rabbit anti-PI3K antibodies (ab151549; 1:1,000), rabbit anti-phosphorylated AKT (p-AKT) antibodies (ab38449; 1:500), rabbit anti-AKT antibodies (ab185633; 1:2,500), and rabbit anti-GAPDH antibodies (ab181603; 1:9,000). The proteins were visualized using enhanced chemiluminescence reagents (Pierce, Rockford, IL, USA).
3
Western Blot Analysis of Apoptosis and Signaling
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Cells were collected and lysed in RIPA lysis buffer (Beyotime) supplemented with protease inhibitors (Roche, Switzerland). The equivalent amounts of proteins (20 µg) were denatured at 100°C in loading buffer for 15 min, resolved on 8–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, and transferred to polyvinylidene fluoride (PVDF) membranes by voltage gradient transfer. The blots were blocked overnight with 5% non-fat milk. The membrane was incubated with primary antibodies and proper secondary antibodies. The used primary antibodies against p53 (ab131442), p21 (ab109199), cyclin D1 (ab134175), caspase-3 (ab4051), cleaved-caspase-3 (ab49822), caspase-9 (ab202068), cleaved-caspase-9 (ab2324), MLK3 (ab51068), Sirt1 (ab110304), AKT (ab185633), p-AKT (ab131443), Wnt3a (ab28472), β-catenin (ab32572), and β-actin (ab8227) purchased from Abcam (UK) were used at the dilution of 1:1000. β-actin was used as a loading control. After washing, the binding antibody was visualized using the enhanced chemiluminescence assay kit (Tiangen Biotechnology Corp., China) according to the manufacturer’s instructions.
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