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Sa00004 1

Manufactured by Proteintech
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SA00004-1 is a laboratory equipment product from Proteintech. It is designed for a core function of biological sample preparation and analysis. Further details on the specific features and capabilities of this product are not available at this time.

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Lab products found in correlation

Ab7959, by Abcam (1 mentions) Fluorescence microscope, by Nikon (1 mentions) Enhanced chemiluminescence, by Bio-Rad (1 mentions) Sa00004 2, by Proteintech (1 mentions) 60004 1 ig, by Proteintech (1 mentions) Ab5535, by Proteintech (1 mentions) Ipvh00010, by Merck Group (1 mentions)

2 protocols using sa00004 1

1

Immunohistochemical Analysis of p-ERK and Cyclin E1

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As previously described (27 (link)), sections were deparaffinized, rehydrated, soaked in an antigen retrieval solution with citrate buffer, and incubated overnight at 4°C with a rabbit polyclonal antibody against p-ERK (9101; 1:200 dilution; Cell Signaling Technology) or cyclin E1 (ab7959; 1:200 dilution; Abcam). Biotinylated secondary antibody (SA00004-1; 1:200 dilution; Proteintech Group, Inc.) was then added and followed by incubation with a streptavidin-peroxidase conjugate for 30 min at room temperature. Images were captured under a fluorescence microscope (Nikon Corporation).
Yu M., Liu X., Wu H., Ni W., Chen S, & Xu Y. (2017). Small interfering RNA against ERK1/2 attenuates cigarette smoke-induced pulmonary vascular remodeling. Experimental and Therapeutic Medicine, 14(5), 4671-4680.
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2

Whole-Cell Protein Extraction and Western Blot Analysis

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For whole-cell protein extraction, liver tissues were prepared in lysis buffer (Beyotime, P0013B). Protein lysates were separated by SDS-PAGE. Next, the gel was transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). After being blocked with 5% skimmed milk, the membranes were incubated overnight with the anti-Sox9 (1:200 dilution Millipore,AB5535), anti-GAPDH (1:500 dilution Proteintech, 60004-I-Ig) at 4°Cfor 12h. Then, the membranes were incubated with the corresponding secondary antibodies (1:1000 dilution Proteintech SA00004-2; 1:1000 dilution Proteintech SA00004-1;) at room temperature for 1.5h. Finally, the membranes were visualized with enhanced chemiluminescence (Bio-Rad, USA).
Liu S., Qin D., Yan Y., Wu J., Meng L., Huang W., Wang L., Chen X, & Zhang L. (2021). Metabolic nuclear receptors coordinate energy metabolism to regulate Sox9+ hepatocyte fate. iScience, 24(9), 103003.
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