I4000sr

The patients’ ages, number of pregnancies, parity, number of surviving children, number of aborti, number of cesareans, week of pregnancy, history of goiter and previous history of hydatidiform mole pregnancy were recorded. Serum samples were studied using an Abbott Architect I4000SR device using standard methods. The patients’ initial TSH, fT4, fT3, total T4 (TT4), total T3 (TT3), beta-HCG, anti-thyroid peroxidase (anti-TPO), anti-thyroglobulin (anti-TG) and thyroglobulin (TG) levels were measured. All patients with euthyroid values were operated at the gynecology and obstetrics clinic.
The patients underwent thyroid ultrasonography at the endocrine clinic using a Siemens-Acuson 7500 device. On thyroid ultrasonography images, the patients’ thyroid size (right lobe, left lobe and isthmus) was measured. Thyroid volume was calculated using the formula: width x length x height x 0.523.¹¹ (link)

Twenty-one PW who were HBeAg-negative chronic ASCs with normal ALT levels were enrolled according to the guidelines for the prevention and treatment of chronic hepatitis B (2019 version) (Cui et al., 2022 (link)), and fifteen sex- and age-matched healthy PW without HBV infection and 13 healthy controls (HC) without pregnancy were also recruited at Women’s Hospital, Zhejiang University School of Medicine (Hangzhou, China) in this study. Individuals were excluded from the study if they had haematological system diseases, infectious diseases, diabetes, autoimmune disease, or other hepatotropic diseases. Chronic HBeAg-negative ASCs with normal ALT levels were also excluded if they accepted HBV treatment within six months before blood sampling, and healthy PW and HC who had a vaccine history within six months were also excluded. Serum levels of 17-β-oestradiol (E2) and progesterone (P4) were detected by chemiluminescent microparticle immunoassay (Abbott i4000SR, USA). The demographic and laboratory characteristics of PW with chronic ASCs, healthy PW and HC are shown in Table 1. This study was approved by the Medical Ethical Committee of the Women’s Hospital, Zhejiang University School of Medicine according to the Declaration of Helsinki (1964).