Ch30 compound microscope

Appendages of a paratype were dissected for description under Olympus SZ51 stereo microscope and drawn under an Olympus CH30 compound microscope with a camera lucida. The holotype dorsal and lateral drawings are based on photos taken by Olympus DP71 microscope digital camera with Olympus SZH10 stereo microscope. Drawings were inked using Adobe Illustrator with Wacom Bamboo drawing tablet. Morphological characters for the descriptions follow Bruce (2004a) , and were prepared using DELTA (Descriptive Language for Taxonomy: Coleman et al. 2010 ; Dallwitz 1980 ; Dallwitz et al. 1997 , 2006 ).
Abbreviations: PSUZC, Prince of Songkla University Zoological Collection; MTQ, Museum of Tropical Queensland. Queensland Museum; PMS, plumose marginal setae; RS, robust seta/setae; CPS, circumplumose setae.

The specimens were preserved in 95% ethanol. Parts of the specimens were dissected and mounted on microscope slides fixed in Euparal or glycerin. Ethanol-preserved specimens were studied using a Nikon SMZ745 stereomicroscope. Drawings of the microscope slides were generated via a camera lucida on an Olympus CH30 compound microscope and subsequently scanned using the Procreate application (iOS application) for illustration. The larvae were captured in photographs using a Nikon Research Stereomicroscope SMZ25 and afterwards processed using NIS-Elements software. The final plates were created and processed using Adobe Photoshop software (http://www.adobe.com). The distribution map was generated with SimpleMappr software (https://simplemappr.net). They were subsequently transferred to absolute ethanol to facilitate the dehydration process to conduct scanning electron microscopy (SEM). The specimens were then dissected, transferred to microtubes, and covered with a fine mesh net (mesh size 60 µm) for drying in a critical point dryer (CPD). The specimens were placed on stubs and coated with a 20-nm gold layer using a Cressington sputter coater. Zeiss LEO 1450 VP was implemented for taking SEM images.