Muc 1 fitc

Manufactured by BD

MUC-1-FITC is a fluorescently labeled antibody that binds to the MUC-1 antigen. It is used in research applications to detect and quantify the expression of the MUC-1 protein.

Automatically generated - may contain errors

Lab products found in correlation

Lsr 2 flow cytometer, by BD (1 mentions) Cd90 apc, by BD (1 mentions) Cd73 pe, by BD (1 mentions) Cd24 pe, by BD (1 mentions) Cd44 apc, by BioLegend (1 mentions) Epcam apc, by Miltenyi Biotec (1 mentions) Cd10 pe, by BD (1 mentions) Cd166 pe, by BD (1 mentions) Epcam pe, by Miltenyi Biotec (1 mentions) Facsverse flow cytometer, by BD (1 mentions) Live dead fixable violet dead cell stain, by Thermo Fisher Scientific (1 mentions)

2 protocols using muc 1 fitc

Multiparametric Flow Cytometric Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adherent cells were collected by trypsinization, stained using antibodies CD49f-APC (FAB13501A), CD140b-FITC (FAB1263F) (R&D Systems), PROCR (CD201)-PE (130-105-256), EpCAM-PE (130-091-253), EpCAM-APC (130-091-254) (Miltenyi Biotech Inc.), CD271-APC (345108) (Biolegend), CD44-APC (559942), CD24-PE (555428), CD73-PE (561014), CD90-APC (559869), CD166-PE (559263), JAM-1-PE (552556), MUC-1-FITC (559774) (BD Pharmingen), CD10-PE (340920) (BD Biosciences), and CD117-FITC (11-1178-42) (eBioscience), and were acquired using a BD LSR II flow cytometer. Data were analyzed using CellQuest or FlowJo software. Forward and side scatter were used to ensure that only live cells were considered in the analysis. Gating was done using appropriate FITC (555573), PE (555749) and APC (555576) (BD Pharmingen) isotype control antibodies and only a representative isotype control for two fluorescent markers are shown.

Prasad M., Kumar B., Bhat-Nakshatri P., Anjanappa M., Sandusky G., Miller K.D., Storniolo A.M, & Nakshatri H. (2019). Dual TGFβ/BMP pathway inhibition enables expansion and characterization of multiple epithelial cell types of the normal and cancerous breast.. Molecular cancer research : MCR, 17(7), 1556-1570.

+ Open protocol

+ Expand

Enzalutamide Modulates Breast Cancer Cell Surface Phenotype

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the effect of enzalutamide on the cell surface phenotype of breast cancer cells, ZR75-1, BT549 and MDA MB 231 cells were treated with either enzalutamide or vehicle for 48 hours. After 48 hours, cells were harvested and stained with the following antibodies: HLA A2-PE-Cy7 (MHC-I), MUC-1-FITC (TAA), CD54-BV421 (ICAM-1), CD95-FITC (Fas) (BD Biosciences, San Jose, CA), CEA-APC (TAA) (Miltenyi Biotec, Auburn, CA), TRAIL receptor 1 and TRAIL receptor 2 (R & D Systems, Minneapolis, MN). LIVE/DEAD Fixable Violet Dead Cell Stain (Life Technologies, Grand Island, NY) was used to determine cell viability. Cells were incubated with the antibodies for 30 min at 4°C, acquired on a FACS Verse flow cytometer (Becton Dickinson, Franklin Lakes, NJ), and analyzed using FlowJo software (TreeStar, Inc., Ashland, OR).

Kwilas A.R., Ardiani A., Gameiro S.R., Richards J., Hall A.B, & Hodge J.W. (2016). Androgen deprivation therapy sensitizes triple negative breast cancer cells to immune-mediated lysis through androgen receptor independent modulation of osteoprotegerin. Oncotarget, 7(17), 23498-23511.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!