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Apc conjugated cd11b

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The APC-conjugated CD11b is a flow cytometry reagent that binds to the CD11b antigen, which is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. The APC fluorescent dye is conjugated to the CD11b antibody, allowing for the detection and quantification of CD11b-expressing cells in a sample.

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Lab products found in correlation

Annexin 5 apoptosis detection kit apc, by Thermo Fisher Scientific (2 mentions) Iba1 antibody, by Fujifilm (1 mentions) Mhcii 1 a 1 e, by BioLegend (1 mentions) Dapi d3571, by Thermo Fisher Scientific (1 mentions) Pe conjugated cd86, by Thermo Fisher Scientific (1 mentions) Fitc conjugated cd3, by Thermo Fisher Scientific (1 mentions) Percp cy5.5 conjugated cd11c, by Thermo Fisher Scientific (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Anti mouse, by R&D Systems (1 mentions) Facsaria 1 cell sorter, by BD (1 mentions) Anti mouse, by Thermo Fisher Scientific (1 mentions) Facscanto 2 analyzer, by BD (1 mentions) Facsdiva 8, by BD (1 mentions) Efluor450 conjugated anti cd45, by Thermo Fisher Scientific (1 mentions) Fitc conjugated anti gr 1, by Thermo Fisher Scientific (1 mentions) Pe conjugated anti f4 80, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by BD (1 mentions)

6 protocols using apc conjugated cd11b

1

Murine Neuroinflammation Immunostaining Protocol

Cited 6 times
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We obtained murine recombinant IL-33 (580506), AF647-conjugated Aβ (clone: 6E10) antibody (803021), APC-conjugated MHC-II (clone: M5/114.15.2) antibody (107614), FITC-conjugated VCAM1 (clone: MVCAM.A) antibody (105706) and MHC-II (I-A/I-E) (clone: M5/114.15.2) antibody (107601) from BioLegend. We obtained ICAM1-neutralizing (clone: YN1/1.7.4) antibody (BE0020)75 (link),76 (link) and VCAM1-neutralizing (clone: M/K-2.7) antibody (BE0027)69 (link),77 (link) from Bio X Cell. We obtained AF488-conjugated CD11b (clone: M1/70) antibody (53-0112-82), APC-conjugated CD11b (clone: M1/70) antibody (17-0112-83) and biotinylated CD11b (clone: M1/70) antibody (13-0112-82) from eBioscience. ApoE-neutralizing (clone: HJ6.3) antibody was a gift from D. Holtzman36 (link). We obtained DAPI (D3571) from Life Technologies, and mouse ITGB2 recombinant protein (LS-G14036-10) was from LSBio. We obtained mouse ApoE recombinant protein (MBS955382) from MyBioSource as well as CCR7 neutralizing (clone: 4B12) antibody (MAB3477)78 (link) and VCAM1 antibody (BBA5) from R&D Systems. We obtained recombinant mouse CD44 protein (53953-M08H) from Sino Biological, MeX04 (4920) from Tocris Bioscience and Iba1 antibody (019-19741) from Wako.
Lau S.F., Wu W., Wong H.Y., Ouyang L., Qiao Y., Xu J., Lau J.H., Wong C., Jiang Y., Holtzman D.M., Fu A.K, & Ip N.Y. (2023). The VCAM1–ApoE pathway directs microglial chemotaxis and alleviates Alzheimer’s disease pathology. Nature Aging, 3(10), 1219-1236.
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2

Tolerogenic Dendritic Cell and T-Cell Analysis

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Directly after euthanasia of the mice, the mesenteric lymph node was placed in ice-cold PBS. Single-cell suspensions were prepared by disrupting the lymph node between two microscope glasses and passing it through a 70 μm nylon mesh. After washing and resuspension, 1 × 106 cells were surface stained for 30 min with antibodies for Percp-Cy5.5 conjugated CD11c, PE-conjugated CD86, APC-conjugated CD11b, and FITC-conjugated CD103 (all antibodies were purchased from eBiosciences) for the detection of tolerogenic dendritic cells (DCs). For the detection of T cell subsets, 1 × 106 cells were initially surface stained for 30 min with FITC-conjugated CD3, PercP-Cy5.5-conjugated CD4, and APC-conjugated CD8a (ebiosciences), then fixate and permeabilized with the FoxP3/Transcription Factor Staining Buffer Set (ebiosciences), and finally stained for 30 min with PE-conjugated intracellular forkhead box P3 (FOXP3) (ebioscience). Analysis was performed using an Accuri C6 flow cytometer (Accuri Cytometers).
Rasmussen T.S., Mentzel C.M., Danielsen M.R., Jakobsen R.R., Zachariassen L.S., Castro Mejia J.L., Brunse A., Hansen L.H., Hansen C.H., Hansen A.K, & Nielsen D.S. (2023). Fecal virome transfer improves proliferation of commensal gut Akkermansia muciniphila and unexpectedly enhances the fertility rate in laboratory mice. Gut Microbes, 15(1), 2208504.
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3

Multiparameter Flow Cytometry Analysis

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Cell suspensions were labelled with anti-mouse phycoerythrin (PE)-conjugated VEGFR1 (#FAB4711P; R&D Systems), anti-mouse PE-F4/80 (#12-4801; eBioscience Inc., CA, USA), anti-mouse PE-VEGFR2 (#12-5821; eBioscience), anti-mouse PE-CD335 (#12-3351; eBioscience), anti-mouse PE-CD202b (TIE2) (#12-3351; eBioscience), anti-mouse PE-Ly-6G (Gr-1) (#12-5931; eBioscience) and anti-mouse allophycocyanin (APC)-conjugated Cd11b (#53-0112; eBioscience) with corresponding isotype control. Then the samples were analysed on BD FACSCanto II Analyzer (BD Biosciences), and cell sorting was performed on BD FACSAria I Cell Sorter (BD Biosciences). The data analysis was carried out by using FlowJo software (Tree Star Inc., Ashland, OR, USA).
Xu W.W., Li B., Guan X.Y., Chung S.K., Wang Y., Yip Y.L., Law S.Y., Chan K.T., Lee N.P., Chan K.W., Xu L.Y., Li E.M., Tsao S.W., He Q.Y, & Cheung A.L. (2017). Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression. Nature Communications, 8, 14399.
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4

Phenotyping tumor-infiltrating immune cells

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Single-cell suspensions from MC38-Vector or MC38-IL33 tumors were incubated with APC-conjugated CD11b, FITC-conjugated anti-Gr1, PE-conjugated anti-F4/80, or eFluor450-conjugated anti-CD45 (all from eBioscience, San Diego, CA). One million cells were incubated with antibodies in 100 μL wash buffer (PBS with 0.1% BSA) at 4°C for 1 h. Cells were washed twice with ice-cold wash buffer and fixed with 1% paraformaldehyde. FACS analyses were performed on a Beckton Dickinson LSRII flow cytometer and analyzed with FACSDiva 8.0 software (BD Biosciences).
Zhang Y., Davis C., Shah S., Hughes D., Ryan J.C., Altomare D, & Peña M.M. (2016). IL-33 Promotes Growth and Liver Metastasis of Colorectal Cancer in Mice by Remodeling the Tumor Microenvironment and Inducing Angiogenesis. Molecular carcinogenesis, 56(1), 272-287.
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5

Assessing Knockout Cell Differentiation and Apoptosis

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Cells were transduced with gRNA-expressing lentivirus and BFP-positive (knock-out) cells were collected by cell sorting. For differentiation analysis, cells were stained with APC-conjugated CD11b (eBioscience) 7 days post transduction. For apoptosis assays, cells were analysed 9 days post transduction using Annexin V Apoptosis Detection Kit APC (eBioscience). Details in Supplementary Methods.
Au Y.Z., Gu M., De Braekeleer E., Gozdecka M., Aspris D., Tarumoto Y., Cooper J., Yu J., Ong S.H., Chen X., Tzelepis K., Huntly B.J., Vassiliou G, & Yusa K. (2020). KAT7 is a genetic vulnerability of acute myeloid leukemias driven by MLL rearrangements. Leukemia, 35(4), 1012-1022.
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6

Assessing Knockout Cell Differentiation and Apoptosis

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Cells were transduced with gRNA-expressing lentivirus and BFP-positive (knock-out) cells were collected by cell sorting. For differentiation analysis, cells were stained with APC-conjugated CD11b (eBioscience) 7 days post transduction. For apoptosis assays, cells were analysed 9 days post transduction using Annexin V Apoptosis Detection Kit APC (eBioscience). Details in Supplementary Methods.
Au Y.Z., Gu M., De Braekeleer E., Gozdecka M., Aspris D., Tarumoto Y., Cooper J., Yu J., Ong S.H., Chen X., Tzelepis K., Huntly B.J., Vassiliou G, & Yusa K. (2020). KAT7 is a genetic vulnerability of acute myeloid leukemias driven by MLL rearrangements. Leukemia, 35(4), 1012-1022.
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