The rat right hind ankle joint was soaked in EDTA decalcifying solution for two months. Immunohistochemistry (IHC) staining was performed according to the protocol in the SP9000 IHC reagents kit (ZSGB Bio, China), and HE staining was performed according to the protocol in the HE staining kit (Beyotime, China). Each sample was observed by a digital pathology slide scanner (3DHISTECH, Hungary). The IHC results were quantitatively analyzed by the Image-ProPlus Software (MEDIA CYBERNETICS, USA) to calculate the integral optical density (IOD).
Digital pathology slide scanner
Manufactured by 3DHISTECH
Sourced in Hungary
The Digital pathology slide scanner is a laboratory equipment designed to digitize glass microscope slides. It captures high-resolution images of the slide contents, allowing for digital storage, analysis, and sharing of the samples.
Automatically generated - may contain errors
4 protocols using digital pathology slide scanner
1
Rat Ankle Joint Decalcification and IHC Analysis
2
Histological Analysis of Liver Tissue
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Liver tissues were fixed with 4% paraformaldehyde for 24 h, then embedded in paraffin blocks and stained with hematoxylin and eosin (H&E). Immunohistochemistry (IHC) was performed according to a standard procedure. The pathological changes were assessed by a digital pathology slide scanner (3 DHISTECH, Hungary). The IHC results were quantitatively analyzed by the Image-ProPlus Software (MEDIA CYBERNETICS, USA) to calculate the integral optical density (IOD).
3
Histological Analysis of Rat Ankle Joints
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Rat secondary ankle joints were harvested, fixed in 4% paraformaldehyde, soaked in 10% ethylenediaminetetraacetic acid, and embedded in paraffin. Immunohistochemistry (IHC) staining was performed using the SP9000 IHC reagent kit (ZSGB Bio, China) according to the manufacturer’s protocol. Hematoxylin and eosin (H&E) staining and toluidine blue staining were performed using the H&E staining kit (Beyotime, China) and toluidine blue according to the manufacturer’s protocol. Serial sections were examined using a digital pathology slide scanner (3DHISTECH, Hungary).
4
Immunohistochemical Analysis of Nesfatin-1 in Synovium
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Due to limitations in the available and small sample volume, 5 synovium samples in each group were harvested, fixed in 4% paraformaldehyde for 48 h, and embedded in paraffin. Serial sections (4 μm) were cut. Immunohistochemistry for nesfatin-1 was performed using the SP-9000 Histostain-Plus kit (ZSGB Bio, China), according to the manufacturer’s instructions. Samples were visualized using a digital pathology slide scanner (3DHISTECH Ltd., The Digital Pathology Company, Budapest, Hungary). Image-ProPlus Software (Media Cybernetics, Silver Spring, MD, USA) was used to calculate the integral optical density of the sections.
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