Human galectin 3 elisa kit

Cells were seeded on a 6-well plate at a density of 3 × 10⁵ cells/well in 1 ml of complete medium in quadruplicate with relevant siRNA transfections and chemical inhibitor treatments. After a 48 h or 72 h incubation, the supernatants (i.e., conditioned medium) were collected and spun down at 3000g for 10 min to remove any cell debris. Supernatants were then analyzed for galectin 3 content using a human galectin 3 ELISA kit (Abcam, ab 188394) as recommended by the manufacturer. Cells in the well were trypsinized to detach and counted. Galectin 3 in the supernatant was normalized to cell number.

A549 and LK-2 cells (2.5×10⁵ and 5×10⁵ cells/10-cm dish, respectively) were cultured under a normoxic or hypoxic condition for 3 days. Cell culture supernatants were then harvested and centrifuged at 15,000 × g for 5 min at 4°C to remove cell debris, and the levels of galectin-3 were measured using the Human Galectin-3 ELISA kit (Abcam, Cambridge, UK) according to the manufacturer's instructions.