Victor3 multilabel plate reader 96 well plates black

Lysozyme activity was determined by a fluorescence-based assay (EnzChek® Lysozyme Assay Kit, Molecular Probes Europe BV, Leiden, The Netherlands) using a suspension of Micrococcus lysodeikticus labeled with fluorescein. The assay determines the lysozyme activity on the cell walls of Micrococcus lysodeikticus, which are labeled to such a degree that fluorescence is quenched. The fluorescence increase was measured using a microplate reader with a fluorescein filter and OptiPlate™-96 F microwell plates (VICTOR3™ Multilabel Plate Reader; 96-Well plates black, PerkinElmer Life and Analytical Sciences, Shelton, Germany). Preparation of DQ Lysozyme substrate stock suspension, lysozyme standard curve, as well as the procedure were conducted according to the manufacturer's protocol. The reaction mixtures were incubated at 37 °C for 30 min, protected from light. The fluorescence intensity of each reaction in a microwell plate was measured at 494 nm (absorption maximum) and 518 nm (emission maximum). The fluorescence values obtained from the control without enzyme were subtracted.

Lysozyme activity was determined by a fluorescence-based assay (EnzChek® Lysozyme Assay Kit, Molecular Probes Europe BV, Leiden, The Netherlands) using a suspension of Micrococcus lysodeikticus labeled with fluorescein. The assay determines the Lysozyme activity on labeled cell walls of Micrococcus lysodeikticus. The fluorescence increase was measured using a microplate reader with a fluorescein filter and OptiPlate™-96 F microwell plates (VICTOR3™ Multilabel Plate Reader; 96-Well plates black, PerkinElmer Life and Analytical Sciences, Shelton, USA). Preparation of DQ Lysozyme substrate stock suspension, Lysozyme standard curve, as well as the procedure were conducted according to the manufacturer's protocol. The reaction mixtures were incubated at 37 °C for 30 min, protected from light. The fluorescence intensity of each reaction in a microwell plate was measured at 494 nm (absorption maximum) and 518 nm (emission maximum). The fluorescence values obtained from the control without enzyme were subtracted.