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21 protocols using anhydrous methanol

1

Synthesis of Tellurium-based Nanoparticles

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Tellurium powder, thioglycolic acid (TGA), CdCl2·2H2O, and sodium borohydride (NaBH4) were supported by Aladdin Reagent Co., Ltd. (Shanghai, China). TEOS, APTES, NaOH, anhydrous methanol, acetone, Triton X-100, Tris (hydroxymethyl) aminomethane, and anhydrous methanol were acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Standards, ferulic acid (FA), chlorogenic acid (CGA), 4-hydroxybenzoic acid (4-HBA), caffeic acid (CA), p-coumaric acid (pCA), vanillic acid (VA), and protocatechuic aldehyde (PA) were ordered from Xiya Reagent Co., Ltd. (Chengdu, China). All stock solutions were prepared with ultrapure water (18.2 MΩ) from a Milli-Q water purification system (Millipore, Bedford, MA, USA).
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2

Antioxidant Evaluation of Sea Buckthorn and Microbial Cultures

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Folin–Ciocalteu reagent and 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox) were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and gallic acid were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). 2,2′–azino-bis-(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ) were purchased from Aladdin Industrial Corporation (Shanghai, China). Ethanol absolute, methanol anhydrous, sodium carbonate, potassium persulfate, glacial acetic acid, sodium acetate, acetic acid, iron trichloride, and hydrochloric acid (HCL) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Sea buckthorn (named “shengqiuhong”, Fructus Hippophae, produced in Tacheng, Xinjiang, China) was provided by Gansu Aikang Sea buckthorn Products Co.,Ltd. (Gansu, China). Lactobacillus paracasei was provided by Xi’an Jushengyuan Biotechnology Co., Ltd.(Xi’an, China). Saccharomyces cerevisiae was provided by Angelyeast Inc.(Hubei, China). Sucrose was from the local market.
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3

Dopamine-loaded Zn-based Metal-Organic Nanoparticles

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Zinc acetate dihydrate (Zn(OAC)2·2H2O, 99.99%) and dopamine hydrochloride (AR, 98.0%,) were purchased from Aladdin Corp. Cetyltrimethylammonium bromide (CTAB, 99%) and triblock poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) Pluronic F-127 (Mav = 12,600) were purchased from Sigma-Aldrich. Tris(hydroxymethyl)aminomethane (Tris, ≥99.5%), 2-methylimidazole (1-MeIM, AR, ≥98.0%), zinc nitrate hexahydrate (Zn(NO3)2·6H2O, AR, ≥99.0%), cobalt (II) nitrate hexahydrate (Co(NO3)2·2H2O, ≥ 98.5%,), 1,3,5-trimethylbenzene (TMB, ≥ 99.5%), hydrochloric acid (HCl, 36.0–38.0%), 2,3,6-trimethylphenol (TMP, 98.0%), methanol anhydrous (AR, ≥99.5%), hydrogen peroxide (30 wt.%), ammonium hydroxide solution (28 wt.% NH3 in H2O), polyvinylpyrrolidone K30 (GR), copper(II) chloride dihydrate(AR, ≥99.0%) and anhydrous ethanol (AR, ≥99.7%) were purchased from Sinopharm Chemical Reagent Co., Ltd. Disulfiram (97%) was purchased from Aladdin Reagent Co. Ltd. Fetal bovine serum (FBS), penicillin-streptomycin, trypsin, and RPMI 1640 medium were provided by Gibco Life Technologies Co. Cell Counting Kit-8 (CCK-8), calcein-AM/propidium iodide (PI) and annexin V-FITC Apoptosis Detection Kit were obtained from Beyotime Biotech (China).
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4

Cellulose Acetate Synthesis and Characterization

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Cellulose acetate (CA, Mn ~30,000) was provided by Sigma-Aldrich (St. Louis, MO, USA). Acetone was purchased from Hengfa Chemical Reagent Co., Ltd. (Tianjin, China). Methanol anhydrous, anhydrous citric acid, sodium citrate, sodium chloride, sodium hydroxide, N, N-Dimethylacetamide (DMAc), and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Anhydrous ethanol was provided by Kermel Chemical Reagent Co., Ltd. (Tianjin, China). Hydrochloric acid was purchased from China Pingmei Shenma Group Kaifeng Dongda Chemical Co., Ltd. (Kaifeng, China).
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5

Synthesis and Characterization of Zinc-Based Photosensitizers

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Zinc acetate (99%), thiourea (AR, 99%), indocyanine green (ICG), iron (III) chloride (FeCl3) and 1,3-diphenylisobenzofuran (DPBF) were purchased from Aladdin Co. Ltd. Polyvinylpyrrolidone (K30), Zinc nitrate hexahydrate (AR), methanol anhydrous, sulfuric acid and sodium acetate anhydrous were obtained from Sinopharm Chemical Reagent Co. Ltd. Tirapazamine and CCK-8 were supplied by Dalian Meilun Biotechnology Co. Ltd. Poly (sodium 4-styrenesulfonate) (PSS, Mw = ~70000), 2-methylimidazole, N, N-Dimethyl-p-phenylenediamine (≥99.0%) and dichlorofluorescein diacetate (DCFH-DA, ≥97%) were purchased from Sigma-Aldrich Co. Ltd.
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6

Jute-based Cellulose Nanofiber Production

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Jute was produced in Bangladesh and purchased from Zhejiang Baisheng Industrial, Taizhou, China (cellulose content is about 67.59%). NMMO aqueous solution (50 wt%) and sodium hypochlorite solution (active chlorine content is 13%) were analytical pure and purchased from McLean, Palm Springs, CA, USA. Bovine serum albumin (BSA) was purchased from Solarbio, Beijing, China. Standard MCC, whose purity is over 99% and average diameter is less than 25 μm, was purchased from Aladdin Chemical Reagents company, Shanghai, China. Hydrogen peroxide (30%, v/v), nitric acid, anhydrous sodium carbonate, glacial acetic acid, anhydrous ethanol, anhydrous methanol, hydrochloric acid (37 wt%), propyl gallate and glycerol (98% purity) were purchased from Sinopharm Group, Beijing, China. All the regents had not been further purified before use.
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7

Fatty Acid and Surfactant Synthesis

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N-Decanoic
acid (99%), lauric acid (98%), myristic acid (99%), palmitic acid
(97%), N,N-dimethylethylenediamine
(98%), dimethyl carbonate (99%), and sodium lauryl benzene sulfonate
(LAS, 95%) were analytical pure and purchased from Shanghai Maclean
Biochemical Technology Co., Ltd. Toluene (99.5%) and anhydrous methanol
(99.5%) were analytical pure and provided by Sinopharm Chemical Reagent
Beijing Co., Ltd. The high-purity water (ρ = 18.25 MΩ·cm–1) was supplied by a Milli-Q ultrapure water purification
system.
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8

Dasatinib-Cisplatin Drug Delivery System

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N, N-Dimethylformamide (DMF) was dried overnight with calcium hydride (98%, Aladdin), then distilled to use. Dasatinib (DAS) (99%) and O-(1H-benzotriazol-1-yl)- N,N,N',N'-tetramethyluronium tetrafluorob-orate (TBTU) (98%) and sodium diethyldithiocarbamate (98%) were obtained from Aladdin (Shanghai, China). Cisplatin (CP) was obtained from Shandong platinum source chemical Co. Ltd (Jinan, China). Anhydrous ethanol, hydrogen peroxide (30%), anhydrous ether, DMF, triethylamine (TEA), succinic anhydridedichloromethane (DCM), anhydrous methanol and pyridine were all purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Cell culture medium, trypsin and fetal bovine serum were bought from KeyGEN BioTECH (Nanjing, China). Poly(2-hydroxyethyl methacrylate) (PHEMA) and 3-(4, 5-dimethylthiazol-2-yl)−2, 5-diphenyl tetrazolium bromide (MTT) were bought from Sigma-Aldrich. Male ICR mice (SPF, 18–22 g) were obtained from Animal Center of Anhui Medical University (Hefei, China).
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9

Synthesis of Ferrosoferric Oxide Nanoparticles

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Ferrosoferric oxide (Fe3O4, 50 nm particle size), anhydrous ferric chloride (FeCl3), 3-phenylglutaric acid, antipyrine, and dimethylformaldehyde (FDA) from Shanghai Aladdin Reagent (Shanghai, China); phenol, 1,2-dichloroethane (DCE), dichloromethane, and anhydrous methanol from Sinopharm Chemical Reagent Company (Shanghai, China); and diclofenac sodium (DFS), 4-acetylaminophenol (AMP), and sodium hydroxide (CaH2) from Shanghai Sahn Chemical Technology company (Shanghai, China). Before the experiment, the DCE and pitch were dried and dehydrated.
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10

Synthesis of Amino-Functionalized MOF

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Zirconium tetrachloride
(ZrCl4, 98%), branched polyethyleneimine (PEI, 99%, Mw = 600, viscous liquid, theoretical amino group
content 32.9 wt %, primary amino group content 12.8 wt %), and glutaraldehyde
(GD, 50%) were purchased from Aladdin Chemical Co. Ltd. China. 2-Aminobenzene-1,4-dicarboxylic
acid (ABDC or H2BDC-NH2, 98%) was supplied by
J&K Chemicals. N,N-dimethylformamide
(DMF), anhydrous ethanol, anhydrous methanol, sodium hydroxide (NaOH),
and hydrochloric acid (HCl, 36.5%) were all analytical grade reagents
purchased from Sinopharm Chem. Reagent Co. Ltd. China. All chemicals
were used as received. Nitrogen (N2, ≥99.5%) and
carbon dioxide (CO2, ≥99.995%) were obtained from
Jingong Specialty Gases Co., Ltd. China.
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