For post infection flow cytometry, fixed iAT2s were either stained for cell surface expression of ACE2 (R&D, #AF933, 4-8 μg/2.5x106 cells) followed by donkey anti-goat IgG-AF647 (Invitrogen, #A21447) or were permeabilized with saponin buffer (Biolegend) then stained with SARS-CoV nucleoprotein (N) antibody (Rockland, #200-401-A50, 1:1000), followed by donkey anti-rabbit IgG-AF488 (Jackson ImmunoResearch, #711-545-152). Gating was based on either mock infected stained controls or infected, isotype-stained controls. Flow cytometry staining was quantified using a Stratedigm S1000EXI and analyzed with FlowJo v10.6.2 (FlowJo, Tree Star Inc). FACS plots shown represent single-cells based on forward-scatter/side-scatter gating.
Donkey anti goat igg af647
Manufactured by Thermo Fisher Scientific
Donkey anti-goat IgG-AF647 is a secondary antibody that binds to goat immunoglobulin G (IgG) and is conjugated to the fluorescent dye Alexa Fluor 647. It is designed for use in various immunoassay and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using donkey anti goat igg af647
1
Quantification of SARS-CoV-2 Infection in iAT2 Cells
2
Transparent Optic Nerve Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
iDISCO was carried out as previously described (http://www.idisco.info )22 (link). The following antibodies were used: goat anti-mouse VEGFR3 (R&D, No. AF743,1:400), rat anti-mouse LYVE1 (R&D, MAB2125,1:400), rabbit anti-mouse LYVE1 (AngioBio, No. 11-034,1:200), mouse anti-human VEGFR3 (Santa Cruz Biotechnology, SC-28297, 1:200), rabbit anti-human LYVE1 (Angio-Proteomie, 102-PA50S, 1:200), goat anti-mouse IgG–AF647 (Invitrogen, A21235, 1:500), donkey anti-goat IgG–AF647 (Invitrogen, A21447, 1:500), goat anti-rabbit IgG–AF555 (Invitrogen, A21428, 1:500). Subsequently, the transparent optic nerves with optic nerve sheaths were imaged using a Leica confocal microscope (Stellaris 8). Three-dimensional rendering was completed using Imaris 8 software (Oxford Instruments).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!