Anti alpha tubulin mouse monoclonal antibody

The following antibodies were used: anti-SMN mouse monoclonal antibody (cat. no. 610647, BD Transduction Laboratories; work dilution for Western blotting, 1:10,000; for immunofluorescence, 1:150); anti-ABC1 mouse monoclonal antibody (cat. no. sc-53482 Santa Cruz Biotechnology, Dallas, TX, USA; work dilution for Western blotting, 1:200); anti-alpha-tubulin mouse monoclonal antibody (cat. no. T6074, Sigma-Aldrich, St. Louis, MO, USA; work dilution for Western blotting, 1:2000). The secondary antibodies conjugated to horseradish peroxidase were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA) and used at a dilution of 1:5000. The Alexa Fluor488-conjugated secondary antibodies were purchased from Thermo Fisher (Waltham, MA, USA), and were used at a dilution of 1:200. Filipin (cat. no. F-9765) was purchased from Sigma-Aldrich.