The in vitro simulated fermentation solutions were shown in Table 5 . A total of three different treatments were included: (i) non-rumen fluid + non-melatonin (CK0); (ii) rumen fluid + non-melatonin (CK1); (iii) rumen fluid + melatonin (MLT). Three replicates for each treatment. The 1.2 g feed provided by Youran Dairy Farm (Inner Mongolia, China; Table 4 ) was weighed and used as fermentation substrates for every fermentation bottle. The standard solutions of MLT (9 ng/mL) were prepared according to Özcan and Bagci (61 (link)) using melatonin powder purchased from Sigma-Aldrich company (Sigma-Aldrich, USA). The rumen fluid was collected from four different directions of rumen of three fistula healthy Holstein cows after 2 h of morning feeding. Artificial saliva in vitro culture experiments were prepared according to Cone et al. (62 (link)). All incubation procedures were performed in a thermostatic water bath shaker (SHA-A, Hengfeng Instrument, China) at 39°C (63 (link)). After 24 h of cultivation, the fermentation bottle was taken out and placed in an ice water bath to stop the fermentation.
Melatonin powder
Manufactured by Merck Group
Sourced in United States
Melatonin powder is a natural product derived from the pineal gland of animals. It is a white or off-white crystalline powder that is used as a research material in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Melatonin, by Carl Roth (1 mentions)
Anhydrous ethanol, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Congo red, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Merck Group (1 mentions)
Thioflavin t tht, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
5 protocols using melatonin powder
1
In Vitro Rumen Fermentation with Melatonin
2
Melatonin's Effects on Microcirculation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To study the effects of melatonin, in total 3.3 mg melatonin [melatonin powder, Sigma-Aldrich, St. Louis, MO, USA; 0.3 mg melatonin diluted in NaCl 0.9% to a total volume of 3 ml, 5 min later followed by 3 mg melatonin diluted in 3 ml NaCl 0.9% containing 5% ethanol (Carl Roth, Karlsruhe, Germany)] was applied to the oral and gastric mucosa, and all variables were recorded for 2.5 h. melatonin was applied next to the site of the microcirculation measurement. The gastric bolus reached the stomach via a gastric tube fixed to the O2C probe, whereas the bolus to the oral mucosa was applied around the measuring point via a perforated silicon tube. As melatonin is poorly soluble in water, we choose the solvent ethanol. melatonin mediated effects on local microcirculation may be superseded by direct effects of ethanol. Therefore, melatonin was applied in two subsequent dosages with the lower concentration dissolved in NaCl 0.9% and the higher dissolved in ethanol. To assess early effects of melatonin on local microcirculation, microcirculatory oxygenation and perfusion were analyzed 5 min after each melatonin application.
3
Amyloid Aggregation Assay Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gold Medal all-purpose flour was purchased from Kroger, Cincinnati OH, USA. Hydrochloric acid (HCl) and sodium dodecyl sulfate (SDS) were purchased from Thermo Fisher Scientific, Waltham MA, USA. Sodium chloride (NaCl), ethanol anhydrous, phosphate buffer saline (PBS) solution, Thioflavin T (ThT), Congo Red, and melatonin powder (>=98% purity) were all purchased from Sigma Aldrich, St. Louis MO, USA.
4
Melatonin Effects on Sirohi Buck Semen
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The bucks were randomly divided into 2 groups with 6 animals in each group for the present experiment in order to study effect of exogenous melatonin administration on freezability of Sirohi buck semen during non-breeding season in Southern Rajasthan. The first group treated (T) animals (n=06) were administered single subcutaneous injection of melatonin (Melatonin powder Sigma, USA dissolved in sterile corn oil) @18mg/50kg body weight as per previously described methods (Chemineau et al., 1995, Kumar and Purohit, 2009, Kumar, 2014) [3, 17, 18] .The second group of bucks (n=6) were given only corn oil subcutaneously and considered as control group (C). The semen collections were done aseptically and hygienically on weekly basis during nonbreeding seasons. The artificial vagina (Danish model) of size 20 cm in length and 4.5 cm in diameter with smooth lining (IMV Pvt. Ltd.) was used for semen collection. The semen collection cup with the freshly collected semen was immediately transferred to the laboratory and immersed in a water bath at 30 ºC. Total 48 semen samples non-breeding season-I and 96 semen samples non-breeding season-II during 4 weeks and 8 weeks were collected from the control (n=6) and treated (n=6) buck group per week in the semen collection area of semen laboratory, in a clean dust-free area.
5
Melatonin Stock Solutions Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A series of Mel stock solutions were prepared by dissolving lyophilized melatonin powder (Sigma Aldrich, USA) in 5% ethanol (v/v) and PBS (Gibco, USA). All other chemicals and reagents used were purchased from Sigma-Aldrich unless stated otherwise.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!