The surface texture and morphology of the casted pure PA-12 and 3D-printed PA-12 were observed using Scanning Electron Microscopy (SEM). Specimens were fixed on metal stubs using double-sided carbon tape, gold sputter coated (BALTEC, SCD 005 Sputter Coater, Scotia, NY, USA), and scanned at an accelerating voltage of 10 keV using JEOL JSM-5500LV (Japan) (n = 3).
Scd 005 sputter coater
Manufactured by JEOL
Sourced in United States
The SCD 005 Sputter Coater is a laboratory equipment designed for depositing thin films of conductive materials onto various substrates. It is a compact and versatile instrument used to prepare samples for analysis in electron microscopy and other surface characterization techniques. The SCD 005 Sputter Coater utilizes a sputtering process to uniformly coat the sample surface with a thin, conductive layer, which enhances the imaging and analysis capabilities of the equipment.
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Lab products found in correlation
2 protocols using scd 005 sputter coater
1
Scanning Electron Microscopy of PA-12
2
Collagen Gel Fixation and SEM Imaging
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Collagen gels with and without BV2 cells were processed based on a modified version of Lizárraga and colleagues
[17 (link)]. Briefly, samples were prefixed in 2.5% v/v glutaraldehyde at 4°C for 4 hrs and washed with 0.1 M sodium cacodylate buffer. After overnight incubation, samples were post-fixated in 1% osmium tetraoxide. Samples were then dehydrated using a graded series of ethanol followed by immersion in 100% acetone. The samples were then transferred to a Baltec CPD 030 Critical Point Dryer for critical point drying. Samples were coated with gold-palladium in a Baltec SCD 005 Sputter Coater and examined under JEOL JSM-6400 SEM.
[17 (link)]. Briefly, samples were prefixed in 2.5% v/v glutaraldehyde at 4°C for 4 hrs and washed with 0.1 M sodium cacodylate buffer. After overnight incubation, samples were post-fixated in 1% osmium tetraoxide. Samples were then dehydrated using a graded series of ethanol followed by immersion in 100% acetone. The samples were then transferred to a Baltec CPD 030 Critical Point Dryer for critical point drying. Samples were coated with gold-palladium in a Baltec SCD 005 Sputter Coater and examined under JEOL JSM-6400 SEM.
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