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3 protocols using potassium persulphate kps

1

Ticagrelor-Loaded Chitosan Nanoparticles

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Ticagrelor (TG) was provided as a gift from CCL Pharmaceuticals Pvt. Ltd., Lahore, Pakistan. Low molecular weight chitosan (CH) (100 kDa, 20–300 cP viscosity, 75–85% deacetylation), acrylic acid, acetonitrile, ammonium hydroxide, ammonium acetate, dithio-bis nitrobenzoic acid (Ellman’s reagent), ethyl 3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDAC), ethanol, methylene-bis acrylamide (MBAA), methanol, potassium persulphate (KPS) and sodium tripolyphosphate (TPP) were purchased from Sigma-Aldrich, USA. Glacial acetic acid (GAA), sodium hydroxide (NaOH), sodium dihydrogen phosphate (NaH2PO4) and, thioglycolic acid (TGA) were purchased from Merck Darmstadt, Germany.
Sodium chloride (NaCl) and hydrochloric acid (HCl) were purchased from Supelco, USA. Distilled water was acquired from the research laboratory of The University of Lahore. All the chemicals and reagents utilized were of analytical grade.
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2

Tamarind-based Doxorubicin Delivery System

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Bis(2-(methacryloyloxyethyl) phosphate (BMEP), acylamidoglycolic acid (AGA) and potassium persulphate (KPS) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Sodium hydroxide and hydrochloride were purchased from S.D Fine Chemical Limited (Mumbai, India). Acrylamide, silver nitrate and potassium dihydrogen phosphate (KH2PO4) were purchased from Merck. Doxorubicin Hydrochloride (DOX) were received from LC laboratories (Woburn, MA, USA). Tamarind seeds were collected from the local market. Double distilled water (DDW) water was used for all experiments.
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3

Synthesis of Fluorescent Thermoresponsive Hydrogels

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N-Isopropylacrylamide (NIPAM, 97%, Aldrich), N,N 0 -methylenebis(acrylamide) (BIS, 99%, Sigma-Aldrich), acrylic acid (AAc, 99.5%, Acros Organics), methacryloxyethyl thiocarbamoyl rhodamine B (MRB, Polysciences Inc.), sodium dodecyl sulphate (SDS, 499%, Duchefa Biochemie), potassium persulphate (KPS, 499%, Sigma-Aldrich), pyrromethene 546 (PM546, Exciton Inc.), dimethyldiethoxysilane (DMDES, 97%, Acros Organics) and ammonia (ammonium hydroxide solution, 28.0-30.0% NH 3 basis, Sigma-Aldrich) were all used as received. Water was purified using a Milli-Q water purification system (resistivity 18.2 MO cm, Millipore).
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