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Oregon green s9104s

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Oregon Green S9104S is a fluorescent dye used for labeling and detection. It has an excitation maximum at 496 nm and an emission maximum at 524 nm, making it suitable for applications involving green fluorescence.

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Lab products found in correlation

Snap cell block, by New England Biolabs (2 mentions) T1895, by Merck Group (2 mentions) Thymidine, by New England Biolabs (2 mentions) Snap cell substrates, by New England Biolabs (2 mentions) Snap cell block s9106s, by Thermo Fisher Scientific (2 mentions) 647 sir s9102s, by Thermo Fisher Scientific (2 mentions) Tmr star s9105s, by Thermo Fisher Scientific (2 mentions) Snap cell 430 s9109s, by Thermo Fisher Scientific (2 mentions) P35g 1.5 14 c, by MatTek (2 mentions)

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Oregon Green (29 citations)

2 protocols using oregon green s9104s

1

Synchronizing Cells and Labeling Mitochondria

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were synchronised with a double thymidine block21 (link)
. After incubation in 2 mM thymidine (Sigma-Aldrich, T1895) for 19h, PBS washes, collection using trypsin-EDTA (Thermo Fisher Scientific, 15400054), and centrifugation, cells were treated with Snap-Cell substrates to label old mitochondria, washed, and plated on cell culture dishes, or poly-L-lysine (Merck Millipore, A-005-C, final concentration 1 μg/ml) coated 35 mm glass bottom dishes (MatTek, P35G-1.5-14-C). After 10 hours, thymidine was added for 17 hours, cells washed and returned to MEGM. Cells divided 21-26h after the second thymidine release 21 (link)
.
Snap-Cell Block or Snap-Cell substrates (New England Biolabs) were applied for 30min at 37°C as indicated, diluted in full MEGM with 0.5% BSA (Thermo Fisher Scientific, 15260037) at 5-10μM (Snap-Cell Block S9106S), 1.5-3μM (Snap-Cell 647-SiR S9102S), 3μM (Snap-Cell TMR-Star S9105S), 5μM (Snap-Cell 430 S9109S), 5μM (Snap-Cell Oregon Green S9104S).
Döhla J., Kuuluvainen E., Gebert N., Amaral A., Englund J.I., Gopalakrishnan S., Konovalova S., Nieminen A.I., Salminen E.S., Muñumer R.T., Ahlqvist K., Yang Y., Bui H., Otonkoski T., Käkelä R., Hietakangas V., Tyynismaa H., Ori A, & Katajisto P. (2022). Metabolic determination of cell fate through selective inheritance of mitochondria. Nature cell biology, 24(2), 148-154.
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2

Synchronizing Cells and Labeling Mitochondria

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were synchronised with a double thymidine block21 (link)
. After incubation in 2 mM thymidine (Sigma-Aldrich, T1895) for 19h, PBS washes, collection using trypsin-EDTA (Thermo Fisher Scientific, 15400054), and centrifugation, cells were treated with Snap-Cell substrates to label old mitochondria, washed, and plated on cell culture dishes, or poly-L-lysine (Merck Millipore, A-005-C, final concentration 1 μg/ml) coated 35 mm glass bottom dishes (MatTek, P35G-1.5-14-C). After 10 hours, thymidine was added for 17 hours, cells washed and returned to MEGM. Cells divided 21-26h after the second thymidine release 21 (link)
.
Snap-Cell Block or Snap-Cell substrates (New England Biolabs) were applied for 30min at 37°C as indicated, diluted in full MEGM with 0.5% BSA (Thermo Fisher Scientific, 15260037) at 5-10μM (Snap-Cell Block S9106S), 1.5-3μM (Snap-Cell 647-SiR S9102S), 3μM (Snap-Cell TMR-Star S9105S), 5μM (Snap-Cell 430 S9109S), 5μM (Snap-Cell Oregon Green S9104S).
Döhla J., Kuuluvainen E., Gebert N., Amaral A., Englund J.I., Gopalakrishnan S., Konovalova S., Nieminen A.I., Salminen E.S., Muñumer R.T., Ahlqvist K., Yang Y., Bui H., Otonkoski T., Käkelä R., Hietakangas V., Tyynismaa H., Ori A, & Katajisto P. (2022). Metabolic determination of cell fate through selective inheritance of mitochondria. Nature cell biology, 24(2), 148-154.
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