Dimethylsulfoxide (DMSO), tetrahydrofuran (THF), dimethyl formamide (DMF) was purchased from China National Medicines Co. Ltd (Shanghai, China). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinimidyl(polyethylene-glycol)-2000] (NHS-PEG-DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N [methoxy(polyethylene glycol)-2000] (PEG-DSPE) were purchased from Xi'an Ruixi Biological Technology Co. Ltd (Xi'an, China). The peptide TMTP1 was purchased from GL Biochem Ltd (Shanghai, China). PVDF membrane was purchased from Millipore Corporation (Bedford, MA, USA). Fetal bovine serum was purchased from Gibco Company (Grand Island, NY, USA). 2′,7′-dichlorofluorescin diacetate (DCFH-DA) and Rose Bengal (RB) were provided by Yeasen Co. Ltd. (Shanghai, China). 9,10-Anthracenediyl-bis(methylene)dim alonic acid (ABDA) and 5-chloromethylfluorescein diacetate (CMFDA) were provided by Sigma-Aldrich (St Louis, MO, USA). Cell Counting Kit-8 (CCK8) was purchased from Beyotime Biotechnology (Shanghai, China).
9 10 anthracenediyl bis methylene dimalonic acid abda
Manufactured by Merck Group
Sourced in United States
9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) is a chemical compound used as a fluorescent probe in various laboratory applications. It is composed of an anthracene core with two malonic acid substituents. ABDA exhibits fluorescent properties and is utilized for specific detection and measurement purposes in research and analytical settings.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
2 7 dichlorofluorescindiacetate dcfh da, by Yeasen (1 mentions)
N isopropylacrylamide nipam, by Merck Group (1 mentions)
N n methylenebisacrylamide bis, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Potassium persulfate k2s2o8, by Merck Group (1 mentions)
Calcein am, by Yeasen (1 mentions)
Annexin 5 fitc, by Yeasen (1 mentions)
Fitc phalloidin, by Yeasen (1 mentions)
Ultrapure water system, by Merck Group (1 mentions)
Methylene blue, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Dojindo Laboratories (1 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc propidium iodide apoptosis detection kit, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Deoxyribonucleic acid sodium salt, by Merck Group (1 mentions)
9 protocols using 9 10 anthracenediyl bis methylene dimalonic acid abda
1
Synthesis and Characterization of Multifunctional Nanomaterials
2
Synthesis of Photosensitizing Polymeric Nanoparticles
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Styrene (ST, Ukraine) of p.a. quality was purified via standard method directly before polymerization. N-isopropylacrylamide (NIPAM, Sigma-Aldrich Inc.), N,N′-methylenebisacrylamide (BIS, Sigma-Aldrich Inc.), potassium persulfate K2S2O8 (KPS, Ukraine), sodium phosphate monobasic dihydrate NaH2PO4 × 2H2O (KPS, Ukraine), were of reagent grade and used without further purification. HPPH (2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-alpha) was purchased from Raybiotech, China. Phosphate-buffered saline with pH 7.4 (PBS) was purchased from Gibco Life Technologies (AG, Switzerland). 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA), anionic surfactant sodium dodecyl sulfate (SDS), chloroform, and dimethyl sulfoxide (DMSO) were purchased from Sigma Aldrich (Saint Louis, MO). Two 2-azaazulene polymethine dyes with different conjugation lengths (JB7-08 and JB17-08) were synthesized as described previously [25 (link)].
3
Fluorescent Probes for Cell Assays
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Detailed material information was provided in our previous article45 (link). Additionally, FITC Phalloidin, Calcein-AM, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), and Annexin V-FITC were provided by yeasen Co. Ltd. (Shanghai, China). 9,10-anthracenediylbis(methylene)dimalonic acid (ABDA) was provided by Sigma-Aldrich.
4
Photocrosslinkable Hydrogel Synthesis
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Poly(ethylene glycol)diacrylate (PEGDA), eosin y, 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluene-sulfonate) (TMPyP), sodium meso-tetraphenylporphine-4,4′,4′′,4′′′-tetrasulfonat (TPPS4), methylene blue, 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) and phosphate buffered saline (PBS) were purchased from Sigma Aldrich (St. Louise, USA).
The photoinitiator 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one (α-HAP) was purchased from IGM Resins B.V. (Waalwijk, the Netherlands). A Merck ultrapure water system was used to purify water. All chemicals were used without further purification.
The photoinitiator 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one (α-HAP) was purchased from IGM Resins B.V. (Waalwijk, the Netherlands). A Merck ultrapure water system was used to purify water. All chemicals were used without further purification.
5
Synthesis and Characterization of Photosensitive Polymers
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The conjugated polymers poly[(9,9'-dioctyl-2,7-divinylene-fluorenylene)-alt-2-methoxy-5-(2-ethyl-hexyloxy)-1,4-phenylene] (PFPV) and poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-(2,1′,3)-thiadazole)] (PFBT) were purchased from Luminescence Technology Corp. and ADS Dyes, Inc., respectively. Tetraphenylporphyrin (TPP) was purchased from Adamas-beta, and bis[3,4,6-trichloro-2-(pentyloxycarbonyl) phenyl] oxalate (CPPO) was supplied by TCI. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) was synthesized according to previously reported methods.1 Cholesterol-PEG-folate (Mw = 2000) was purchased from Xi'an Ruixi Biological Technology Co., Ltd. 9,10-Anthracenediyl-bis(methylene) dimalonic acid (ABDA) and 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich. 4',6-Diamidino-2-phenylindole (DAPI) and Cell Counting Kit-8 (CCK-8) were obtained from Dojindo Molecular Technologies. The LIVE/DEAD Viability/Cytotoxicity Kit and Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit were provided by Invitrogen. All other chemicals, if not specified, were used as received without further purification.
6
Analyzing Nucleic Acid Components
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Adenine, guanine, cytosine, thymine, adenosine, 2′-deoxyadenosine 5′-monophosphate, 2′-deoxyguanosine 5′-monophosphate, 2′-deoxycytidine 5′-monophosphate, 2′-deoxythymidine 5′-monophosphate, adenosine 5′-diphosphate, deoxyadenosine 5′-triphosphate, deoxyribonucleic acid sodium salt from salmon testes (approximately 2000 bp), 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich. Deionized (DI) water with a specific resistance of 18.2 MΩ was used.
7
Establishing Murine Breast Cancer Model
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1-Vinylimidazole (VIm), acrylonitrile (AN), acrylamide (AAm), 2, 2'-azoisobutyronitrile (AIBN), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), Hoechst 33342, IR780, N-hydroxysuccinimide (NHS), 2-aminoethanethiol hydrochloride (AET‧HCl), 9,10-anthracenediyl-bis(methylene) dimalonic acid (ABDA) and MTT were obtained from Sigma. Methoxy poly(ethylene glycol) carboxyl acid (mPEG-COOH, 5 kDa) was obtained from Yare Bio-Technology Co. Ltd. (China). DOX was obtained from Beijing Huafeng United Technology Co., Ltd. (China).
The protocol for animal experiments was approved by the Animal Experimentation Ethics Committee of Xi'an Jiaotong University. In order to obtain mice bearing 4T1 tumors, approximately 1×106 4T1 cells (murine breast cancer cells) were inoculated subcutaneously into the flank of female balb/c mice (4 weeks old). The length and width of tumor were measured and the following formula was employed to calculate the tumor volume: volume = 0.5 × width2 × length.
The protocol for animal experiments was approved by the Animal Experimentation Ethics Committee of Xi'an Jiaotong University. In order to obtain mice bearing 4T1 tumors, approximately 1×106 4T1 cells (murine breast cancer cells) were inoculated subcutaneously into the flank of female balb/c mice (4 weeks old). The length and width of tumor were measured and the following formula was employed to calculate the tumor volume: volume = 0.5 × width2 × length.
8
Reagents for Photodynamic Therapy Research
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KMnO4 (≥ 99%) (223,468, sigma), dopamine hydrochloride (DA·HCl, 98%) (1,225,204, sigma), hydrogen peroxide (H2O2, 30%) (349,887, sigma), polyvinyl pyrrolidone (PVP, 98%, Mw = 8,000 Da) (P110608, Aladdin), formamide (F7503, sigma) (99%), and 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) (75,068, sigma) were purchased from Sigma-Aldrich. Chlorin e6 (Ce6, 98%) (R-GMJCe6, Ruixi) was supplied by Xi’an Ruixi Biological Technology. Ethanol (≥ 99.7%) (10,009,218, Sinopharm) and dimethyl sulfoxide (DMSO, > 99.8%) (30,072,418, Sinopharm) were purchased from Sinopharm Chemical Reagent Company. Sorafenib (SOR, 99.5%) (S125098, Aladdin), FA-PEG-SH (Mw = 3,400 Da, 95%) (F163751, Aladdin) and HO-PEG-SH (Mw = 3,400 Da, 95%) (T164376, Aladdin) were supplied by Shanghai Aladdin Chemistry Company. Aqueous dilutions were prepared with deionized H2O. All other chemicals and reagents were commercially available and were used as received.
9
Synthesis and Purification of Novel Compounds
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NRBr and AzBBr were synthesized in our laboratory according to the procedure previously described [19, 20] .
AA, N,N,N´,N´-tetraethylmethylenediamine (TEMED), ammonium persulfate (APS), polyethylene glycol dodecyl ether (Brij 30), AHM, dioctyl sulfosuccinate (AOT), APMA, HEMA, DAT, and 9,10-anthracenediyl-bis (methylene)dimalonic acid (ABDA) were purchased from Sigma-Aldrich.
Hexane, N,N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), and ethanol were obtained from Sintorgan. Trypticase soy agar (TSA) was obtained from Britania (Buenos Aires, Argentina).
Purification by dialysis was performed with cellulose ester membranes (MWCO 50 KDa; SpectrumLabs).
Phosphate-buffered saline (PBS, 10 mM pH 7.4) solution was prepared using sodium chloride, potassium chloride, sodium hydrogen phosphate dihydrate, and potassium dihydrophosphate. All the chemicals were of the highest purity commercially available (Cicarelli) and the solutions were prepared using ultrapure water from a Milli-Q® purification system.
AA, N,N,N´,N´-tetraethylmethylenediamine (TEMED), ammonium persulfate (APS), polyethylene glycol dodecyl ether (Brij 30), AHM, dioctyl sulfosuccinate (AOT), APMA, HEMA, DAT, and 9,10-anthracenediyl-bis (methylene)dimalonic acid (ABDA) were purchased from Sigma-Aldrich.
Hexane, N,N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), and ethanol were obtained from Sintorgan. Trypticase soy agar (TSA) was obtained from Britania (Buenos Aires, Argentina).
Purification by dialysis was performed with cellulose ester membranes (MWCO 50 KDa; SpectrumLabs).
Phosphate-buffered saline (PBS, 10 mM pH 7.4) solution was prepared using sodium chloride, potassium chloride, sodium hydrogen phosphate dihydrate, and potassium dihydrophosphate. All the chemicals were of the highest purity commercially available (Cicarelli) and the solutions were prepared using ultrapure water from a Milli-Q® purification system.
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