Aurora 5 l spectral flow cytometer

Manufactured by Cytek

The Aurora 5 L is a spectral flow cytometer designed for advanced cell analysis. It utilizes multiple lasers and detectors to capture a broad spectrum of cellular information simultaneously. The core function of the Aurora 5 L is to provide high-dimensional, multiparametric data on individual cells within a sample.

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Lab products found in correlation

Live dead fixable near ir cell stain kit, by Thermo Fisher Scientific (2 mentions) Lsrfortessa analyzer, by BD (2 mentions) Trustain fcx plus anti mouse cd16 32 antibody, by BioLegend (1 mentions) Thiazole orange, by Merck Group (1 mentions) Anti ly6g clone 1a8, by BioLegend (1 mentions) Fcs express 7, by De Novo Software (1 mentions) Mouse fc block, by BD (1 mentions) Anti ia ie clone m5 114, by BD (1 mentions) Sp6800 spectral analyzer, by Sony (1 mentions) Anti cd69 clone h1.2f3, by BioLegend (1 mentions) Anti cd4 clone gk1, by Thermo Fisher Scientific (1 mentions) Anti cd11c clone hl3, by BD (1 mentions)

Spelling variants of this product

Cytek Aurora flow cytometer (114 citations) Aurora spectral flow cytometer (79 citations) Aurora spectral cytometer (51 citations) Cytek Aurora cytometer (41 citations) Spectral flow cytometer (6 citations) Aurora 5 l (4 citations)

2 protocols using aurora 5 l spectral flow cytometer

Multiparameter Flow Cytometry Analysis

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In all flow cytometry experiments, except for flow cytometric analysis of RBCs, live dead staining was performed using the LIVE/DEAD Fixable Near-IR cell stain kit (Invitrogen) as described by the manufacturer. Fc receptor blockade was performed by preincubating cells with TruStain FcX PLUS (anti-mouse CD16/32) antibodies (BioLegend). Multiparameter analysis was performed with an LSR Fortessa analyzer (BD Biosciences) or an Aurora 5 L spectral flow cytometer (Cytek). The autofluorescence of cells was subtracted in all experiments using a spectral flow cytometer. Data were analyzed using FlowJo software (V.10.7.1) and flow cytometry software express V.6 (De Novo software).

Pfefferlé M., Dubach I.L., Buzzi R.M., Dürst E., Schulthess-Lutz N., Baselgia L., Hansen K., Imhof L., Koernig S., Le Roy D., Roger T., Humar R., Schaer D.J, & Vallelian F. (2023). Antibody-induced erythrophagocyte reprogramming of Kupffer cells prevents anti-CD40 cancer immunotherapy-associated liver toxicity. Journal for Immunotherapy of Cancer, 11(1), e005718.

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Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were preincubated with a LIVE/DEAD Fixable Near-IR cell stain kit (Invitrogen) and with Mouse BD Fc Block™ (≤1 μg/million cells in 100 μl, BD Biosciences) at 4 °C for 10 min.
The following antibodies were purchased from BD Bioscience: anti-CD45 (clone 30-F11), anti-CD19 (clone 1D3), anti-CD3 (clone 17A2), anti-CD25 (clone 7D4), anti-CD11c (clone HL3), and anti-I-A/I-E (clone M5/114.15.2). The following antibodies were purchased from BioLegend: anti-CD45.1 (clone A20), anti-CD45.2 (clone 104), anti-CD4 (clone GK1.5), anti-CD8 (clone 53-6.7), anti-CD11b (clone M1/70), anti-CD115 (AFS98), anti-CD11c (clone N418), F4/80 (clone BM8), anti-Ly-6G (clone 1A8), and anti-CD69 (clone H1.2F3). The following antibody was purchased from eBioscience: anti-CD4 (clone GK1.5). Corresponding isotype-matched irrelevant specificity controls were purchased from BD, BioLegend, and eBioscience. Reticulocytes were stained with thiazole-orange (Sigma-aldrich). Multiparameter analysis was performed with an LSRFortessa analyzer (BD Biosciences), a SP6800 Spectral Analyzer (Sony) or an Aurora 5 L spectral flow cytometer (Cytek). The data were analyzed using FlowJo software (version 10.7.1) and FCS express 7 (De Novo software).

Vallelian F., Buzzi R.M., Pfefferlé M., Yalamanoglu A., Dubach I.L., Wassmer A., Gentinetta T., Hansen K., Humar R., Schulthess N., Schaer C.A, & Schaer D.J. (2022). Heme-stress activated NRF2 skews fate trajectories of bone marrow cells from dendritic cells towards red pulp-like macrophages in hemolytic anemia. Cell Death and Differentiation, 29(8), 1450-1465.

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