Ftc236

The human FTC cell line, FTC236, was purchased from European Collection of Cell Cultures (ECACC) through Sigma-Aldrich (St. Louis, MO) and maintained in Dulbecco’s Modified Eagle/Ham’s F-12 medium (1:1; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum, 0.01 IU/mL thyroid-stimulating hormone, 40 μg/mL insulin (Sigma-Aldrich), and 50 μg/mL penicillin/streptomycin (Invitrogen).
HTh7, a human-derived anaplastic thyroid cancer (ATC) cell line authenticated by DNA profiling, was provided kindly by Dr Rebecca Schweppe, (University of Colorado, Denver, CO) and maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (Sigma-Aldrich) and 50 μg/mL penicillin/streptomycin (Invitrogen). Both cell lines were maintained in a humidified atmosphere of 5% CO₂ in air at 37°C.

Four human DTC cell lines used in this study were as following: the papillary cancer cell lines TPC1 and BCPAP were kindly provided by Dr. Daniel Ruan (Brigham and Women’s Hospital, Boston, MA) and Dr. Rebecca Schweppe (University of Colorado Denver, CO), respectively; the follicular cancer cell lines FTC133 and FTC236 were purchased from European Collection of Cell Cultures (ECACC) through Sigma-Aldrich (St Louis, MO). TPC1 and BCPAP were maintained in RPMI-1640 (Invitrogen Life Technologies, Carlsbad, CA) medium supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich). FTC133 and FTC236 were maintained in DMEM/Ham’s F-12 (1:1; Invitrogen) medium supplemented with 10% FBS, 0.01 U/mL of thyroid-stimulating hormone (TSH) and 10 μg/mL of insulin (Sigma-Aldrich). All cell lines were grown at 37 °C in a humidified atmosphere containing 5% CO2. To verify the authenticity of the cell lines, DNA short tandem repeat STR analysis was performed by either DDC (DNA Diagnostics Center, Fairfield, OH) (24 (link)) or ECACC. All four cell lines were tested and were found to be authentic.