Hep3b

Five commercial human HCC cell lines (HuH7, HepG2, Hep3B, PLC/PFR/5 and SNU475), purchased from the Japanese Collection of Research Bioresources (JCRB) or the American Type Cell Collection (ATCC), and one primary HCC cell line, obtained in our laboratory (Hcc-1) [19] (link), were cultured in IMDM+GlutaMAX supplemented with 10% FBS, 1% penicillin-streptomycin and 1% non-essential amino acids (Invitrogen, Life Technologies, Milan, Italy) on collagen type I-coated flasks or multi-well plates. The cells were maintained at 37°C in a humidified incubator containing 5% CO₂.

HepG2, Hep3B, HLE, HLF, HuCCT1, and HuH28 cells were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan). Plat-E cells were obtained from Cell Biolabs Inc. (San Diego, CA). HeLa, NIH/3T3, TALDO1 KO, HepG2, Hep3B, HCE, and HCF cells were cultured in Dulbecco’s modified Eagle’s Medium (DMEM; Sigma-Aldrich, St. Louis, MO) supplemented with 10% foetal bovine serum (FBS). Plat-E cells were cultured in DMEM containing 10% FBS, puromycin (1 μg/mL), and blasticidin S (10 μg/mL). HuCCT1 and HuH28 were cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% FBS. The cells were maintained in a 37 °C incubator with 10% CO₂-humidified air.

The HCC cell lines: Huh7, HepG2 and Hep3B were obtained from Japanese Collection of Research Bioresources (National Institute of Health Sciences; Japan, JCRB) and maintained in Dulbecco’s Modified Eagle Medium-High Glucose (Invitrogen) medium with 10% fetal bovine serum (FBS), 2mM L-glutamine (Invitrogen), and 100 μg/mL penicillin-streptomycin (Invitrogen). Cells were cultured in a humidified atmosphere in 5% CO₂ at 37°C.

HEK-293T, NCI-H1299, BxPC-3, PANC-1, Hep3B, PLC/PRF/5,HepG2, SK-HEP-1, MCF7, A549, NCI-H460, Tera-1 and Tera-2 were purchased from ATCC; HuH-7 was purchased from Japanese Collection of Research Bioresources (JCRB), SMMC-7721 and BEL-7402 were purchased from Typical culture preservation commission cell bank, Chinese academy of sciences (NCB); MHCC-97L and LM3 were gifts from Zhongshan Hospital, Fudan University (Shanghai, China); SMMC-7721, BEL-7402, MHCC-97L and LM3 used in this study have been described in previous publication [24] (link), [30] (link), [31] , [32] (link).
HEK-293T, NCI-H1299, BxPC3, PANC1, Hep3B, PLC/PRF/5, HepG2, HuH-7, HepG2, SK-HEP-1, SMMC-7721, 97L, LM3, BEL-7402 and MCF7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum(FBS). A549 and NCI-H460 cells were cultured in RPMI1640 medium supplemented with 10% FBS. Tera1 and Tera2 cells were cultured in McCoy’s 5a medium supplemented with 15%FBS. All cell lines were cultured in the presence of antibiotics at 37°C with 5%CO₂.

The HCC cell lines, Huh7, Huh1, HLE, HLF, and Hep3B, were supplied by the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) or the American Type Culture Collection (Manassas, VA, USA). These cells were maintained at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco).