Animals were intraperitoneally injected with the H3R antagonist thioperamide (2, 5, or 10 mg/kg/day, Sigma-Aldrich, USA) within 1 h of and then once per day after TBI. The H1R antagonist pyrilamine (10 mg/kg/day, Sigma-Aldrich, USA) and H2R antagonist cimetidine (10 mg/kg/day, Sigma-Aldrich, USA) were intraperitoneally injected 15 min before treatment with thioperamide. α-FMH (5 μg/day in saline, intracerebroventricularly [i.c.v.]) (Provensi et al., 2014 (link)) or the H3R agonist immepip (1 μg/day in saline, Sigma-Aldrich, USA, i.c.v.) was given at 15 min before treatment with thioperamide.
Thioperamide
Manufactured by Merck Group
Sourced in United States
Thioperamide is a laboratory equipment product manufactured by Merck Group. It is a chemical compound used for research purposes in various scientific fields. The core function of Thioperamide is to serve as a tool for researchers and scientists in their experiments and investigations.
Automatically generated - may contain errors
Lab products found in correlation
Cimetidine, by Merck Group (2 mentions)
Carboxymethylcellulose, by Merck Group (1 mentions)
β eudesmol, by Fujifilm (1 mentions)
Immepip, by Merck Group (1 mentions)
Pyrilamine, by Merck Group (1 mentions)
Isoproterenol, by Merck Group (1 mentions)
Clobenpropit, by Bio-Techne (1 mentions)
Imetit, by Merck Group (1 mentions)
Histamine, by Merck Group (1 mentions)
35s gtpγs, by PerkinElmer (1 mentions)
4 protocols using thioperamide
1
Histamine Receptor Modulation in TBI
2
Rat TRPA1 Channel Expression
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β-Eudesmol was purchased from Wako Pure Chemical Industries (Osaka, Japan). Thioperamide and carboxymethylcellulose were purchased from Sigma-Aldrich (MO, USA) and Calbiochem (MA, USA). Rat TRPA1 channel expression vector was purchased from OriGene Technologies (MD, USA).
3
Histamine Receptor Antagonists Effects
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The H1 receptor antagonists pyrilamine (30 nM, Cayman Chemicals, Michigan, USA), fexofenadine (1 µM, Cayman Chemicals, Michigan, USA) and cyproheptadine (30 nM, Cayman Chemicals, Michigan, USA), the H2 receptor antagonist cimetidine (1 µM, Sigma Aldrich, Missouri, USA) and the dual H3&H4 receptor antagonist thioperamide (1 µM, Sigma Aldrich, Missouri, USA) were separately applied to tissues. After 30-minute incubation with antagonists, a single dose of histamine (100 µM) was applied to the tissues. In another group of experiments, a combination of antagonists was used. Histamine responses to pyrilamine (30 nM) treated tissues were compared with a combination of pyrilamine (30 nM) and cimetidine (1 µM) treated tissues; cimetidine (1 µM) treated tissues were compared with a combination of pyrilamine (30 nM) and cimetidine (1 µM) treated tissues; combination of pyrilamine (30 nM) and cimetidine (1 µM) treated tissues were compared with a combination of pyrilamine (30 nM), cimetidine (1 µM) and thioperamide (1 µM) treated tissues.
4
Radiolabeled Agonist Preparation and Use
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[125I]Iodoproxyfan (2000 Ci/mmol) was prepared as described [88 (link),89 (link)]. [35S]GTPγS (1250 Ci/mmol) was from Perkin Elmer Life Sciences (Boston, MA, USA). Clobenpropit was from Tocris (Bristol, UK). R-α-methylHistamine, ciproxifan, and proxyfan were provided by W. Schunack (Freie Universität Berlin, Berlin, Germany). Histamine, imetit, thioperamide, and isoproterenol were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). All other chemicals were from commercial sources and were of the highest purity available. Interventionary studies involving animals or humans and other studies that require ethical approval must list the authority that provides the approval and the corresponding ethical approval code.
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