The human benign prostatic epithelial RWPE1 cell line (ATCC CRL-11609) was cultivated in a Keratinocyte serum-free medium (Cat. No. 17005–042) supplemented with BPE (bovine pituitary extract) and EGF (epidermal growth factor) provided with the medium and antibiotics (penicillin 100 U·mL−1, streptomycin 100 μg·mL−1). The human prostate carcinoma 22Rv1 cell line (ATCC CRL-2505) was cultured in an ATCC-formulated Roswell Park Memorial Institute (RPMI)-1640 medium (ATCC 30–2001) supplemented with 10% exosome-depleted foetal bovine serum (A2720801) and antibiotics (penicillin 100 U·mL−1, streptomycin 100 μg·mL−1). Cell lines were purchased from the American Type of Culture Collection (ATCC, Manassas, VA, USA). The cells were maintained at 37 °C in a humidified atmosphere of 5% CO2. The culture media, foetal bovine serum (FBS), antibiotics and other chemicals used for cell cultivation were purchased from GIBCO (Thermo Fisher Scientific, Waltham, MA, USA).
Rwpe 1 cell line
Manufactured by American Type Culture Collection
Sourced in United States
The RWPE-1 cell line is a normal human prostatic epithelial cell line derived from the peripheral zone of the prostate. It is commonly used as a non-tumorigenic control in prostate cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Epidermal growth factor (egf), by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Bovine pituitary extract, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Du145 cells, by American Type Culture Collection (2 mentions)
Du145 cells, by Thermo Fisher Scientific (2 mentions)
Lncap, by American Type Culture Collection (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
Keratinocyte sfm medium, by Thermo Fisher Scientific (1 mentions)
Du 145 pca cells, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Stromal cell basal medium, by Lonza (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Rpmi medium without phenol red, by Merck Group (1 mentions)
Rwpe 1, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Euroclone (1 mentions)
Htb 37, by American Type Culture Collection (1 mentions)
Minimum essential medium (mem), by Euroclone (1 mentions)
11 protocols using rwpe 1 cell line
1
Culturing Prostate Cell Lines
2
Culturing Prostate Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PTEN-deficient LNCaP and PC3 PCa cells and PTEN-wild type DU145 PCa cells were from American Type Culture Collection (ATCC, Manassas, VA, USA), and they were cultured in RPMI medium supplemented with 7.5% FBS, glutamine and antibiotics, in a humidified atmosphere of 5% CO2/95% air at 37 °C. Normal prostate epithelial RWPE-1 cell line was also from ATCC and was grown in keratinocyte-SFM medium supplemented with bovine pituitary extracts and EGF (2.5 μM) (Thermo Fisher Scientific, Waltham, MA, USA). Original stocks of cells were stored frozen in liquid nitrogen; after resuscitation, cells were kept in culture for no more than 10–12 weeks. Cells were detached through trypsin-EDTA solution and passaged once/week.
3
Culturing DU145 and RWPE-1 Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DU145 cells were purchased from the American Type Culture Collection (ATCC, RRID:CVCL_0105 ). All cells used in this study were within 20 passages after thawing. DU145 cells were cultured (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Gibco) and penicillin/streptomycin (1%, Gibco). The RWPE-1 cell line was obtained from ATCC (CRL-11609; RRID:CVCL_3791 ). RWPE-1 cells were maintained in KSFM (Life Technologies) supplemented with 5 ng/mL epidermal growth factor (Life Technologies), 50 mg/mL bovine pituitary extract (Life Technologies), and 1% penicillin-streptomycin (Life Technologies). The cells were routinely cultured in a humidified atmosphere with 5% CO2 at 37°C. All cell lines were found to be Mycoplasma free.
4
Prostate Cancer Cell Line Culturing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LNCaP, VCaP and PC3 cells were purchased from ATCC (Manassas, United States). Cells were grown in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with fetal bovine serum (FBS) (BioWest, South American origin), at 10% in 37 °C and 5% CO2 atmosphere. RWPE-1 cell line was acquired from ATCC and was cultured in Keratinocyte Serum Free Medium (Lonza, Allendale, NJ, USA), PrSC cell line was purchased from LONZA and was cultured in Stromal Cell Basal Medium (LONZA). When cells were treated with DHT hormone, RPMI medium without phenol red (Sigma) supplemented with 5% of charcoal stripped FBS (LONZA) was used.
5
Culturing Prostate, Caco-2, and HEp-2 Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The RWPE-1 cell line (derived from prostate epithelial cells isolated from the peripheral zone of a nonneoplastic human prostate and immortalized with human papillomavirus 18) were purchased from ATCC (Manassas, VA). These cells mimic normal prostate epithelial cell behavior in their response to growth factors and in the expression of prostate-specific antigen (PSA) and androgen receptor. RWPE-1 cells were maintained in a humidified atmosphere of 5% CO2 at 37°C in 1% in keratinocyte serum-free medium (K-SFM) (Gibco, Life Technologies) supplemented with 0.05 mg/ml bovine pituitary extract (BPE), 5 ng/ml human recombinant epidermal growth factor (EGF), and 20 μg/ml gentamicin. Human Caco-2 (ATCC HTB-37) cells were grown in minimum essential medium (MEM [Euroclone, Milan, Italy]). HEp-2 cells (ATCC CCL-23) were maintained in Eagle's minimal essential medium (EMEM [Sigma, Italy]). Both lines were supplemented with 5% heat-inactivated fetal calf serum (FCS [Euroclone, Italy]) and 1% penicillin-streptomycin.
6
Prostate Cancer Cell Culture Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DU145 cells were purchased from the American Type Culture Collection (ATCC). All cells used in this study were within 20 passages after thawing. DU145 cells were cultured (37°C, 5% CO2) in Dulbecco's Modified Eagle's Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Gibco) and Penicillin/Streptomycin (1%, Gibco). The RWPE-1 cell line was obtained from ATCC (CRL-11609). RWPE-1 cells were maintained in KSFM (Life Technologies) supplemented with 5 ng/mL Epidermal Growth Factor (Life Technologies), 50 mg/mL Bovine Pituitary Extract (Life Technologies) and 1% Penicillin-Streptomycin (Life Technologies). The cells were routinely cultured in a humidified atmosphere with 5% CO2 at 37 °C.
7
Establishing YAP-overexpressing BPH-1 Cell Line
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The BPH-1 cell line was purchased from KeyGEN (KG1008, KeyGen Biotech Co., Ltd., NJ, China) and cultured in Roswell Park Memorial Institute (RPMI)-1640 medium with 10% fetal bovine serum (Gibco) supplemented with 1% penicillin and streptomycin. The RWPE-1 cell line was purchased from the American Type Culture Collection and cultured in complete keratinocyte serum-free medium (Invitrogen, USA). All cells were cultured in a humidified atmosphere with 5% CO2 maintained at 37°C and tested for mycoplasma on a regular basis. All cell lines were regularly tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma).
For overexpression of YAP, recombinant pLV-MCS-3FLAG-YAP-SV40-EGFP-IRES-puromycin, the corresponding control plasmid vectors and the lentivirus were produced by Shanghai GeneChem Co., Ltd. The stable cell line was established by lentivirus infection according to the multiplicity of infection (MOI) value. The stable YAP-overexpressing BPH-1 cell line was selected with puromycin (1 μg/mL).
For overexpression of YAP, recombinant pLV-MCS-3FLAG-YAP-SV40-EGFP-IRES-puromycin, the corresponding control plasmid vectors and the lentivirus were produced by Shanghai GeneChem Co., Ltd. The stable cell line was established by lentivirus infection according to the multiplicity of infection (MOI) value. The stable YAP-overexpressing BPH-1 cell line was selected with puromycin (1 μg/mL).
8
Cultivation of Prostate Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human prostatic carcinoma LNCaP, PC-3 and DU 145 cell lines and the immortalized human prostatic epithelial RWPE-1 cell line were obtained from the American Type Culture Collection (Manassas, VA, USA). LNCaP, PC-3 and DU 145 cells were maintained in RPMI-1640 medium (Cellgro; Corning Life Sciences, Corning, NY, USA) supplemented with 10% fetal bovine serum (FBS; Biowest USA, Riverside, MO, USA). RWPE-1 cells were maintained in keratinocyte serum-free medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA). All cells were incubated in an environment of 5% CO2 at 37°C.
9
Culture of Prostate Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The RWPE-1 cell line (normal human prostatic epithelial cells) and LNCap cell line (human prostatic adenocarcinoma cells) were obtained from the American Type Culture Collection. RWPE-1 cells were cultured in keratinocyte serum-free medium supplemented with 0.05 mg mL−1 bovine pituitary extract, 5 ng mL−1 epidermal growth factor, and 1% (v/v) antibiotics (100 U mL−1 penicillin and 100 μg mL−1 streptomycin). LNCap cells were cultured in RPMI1640 containing 10% (v/v) fetal bovine serum and 1% (v/v) antibiotics (100 U mL−1 penicillin and 100 μg mL−1 streptomycin).
10
Prostate Cancer Cell Line Coculture and Irradiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human AR‐positive PCa LNCaP and 22Rv1 cell lines, AR‐negative PCa PC‐3 and DU‐145 cell lines, human prostatic stromal myofibroblast WPMY‐1 cell line, and human prostatic viral immortalized epithelial RWPE‐1 cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). PCa cell lines were cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA), and WPMY‐1 and RWPE‐1 cell lines were cultured in Dulbecco's modified Eagle's medium (DMED) medium, supplemented with 10% foetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) in a humidified atmosphere of 5% CO2 at 37°C. PCa cells were cocultured with sEVs prior to IR treatment using an X‐ray machine (Rad Source RS2000 X‐ray, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!