Cytokine levels in culture supernatants were measured by enzyme-linked immunosorbent assays (ELISA) using an OptEIA mouse TNF-α set (BD Bioscience Pharmingen, CA, USA), a mouse IL-6, adiponectin set, and an IL-4 kit (R&D Systems, Minneapolis, MN). Values for cytokine levels were derived from standard curves using the curve-fitting program SOFTmax (Molecular Devices, Sunnyvale, CA, USA).
Il 4 kit
Manufactured by R&D Systems
Sourced in United States
The IL-4 kit is a laboratory reagent used for the detection and quantification of the cytokine interleukin-4 (IL-4) in biological samples. It provides a standardized and reliable method for measuring IL-4 levels, which is important for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using il 4 kit
1
Measuring Cytokine Levels by ELISA
2
Cytokine Signaling Modulation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NH4F (Macklin, China). Polyethylene glycol (Sinopharm, China). Mouse IL-4 protein (R&D, USA). Heparin (Sangon Biotech, China). Carboxymethyl chitosan and Genipin (Aladdin, China). Matrigel (BD Biosciences, USA). IL-4 Kit, IL-1 Kit, IL-6 Kit, IL-10 Kit and TNF-α Kit were purchased from R&D (USA). Heparin sodium test Kit (Leagene Biotechnology, China).
3
Rabbit Model of Endotoxin-Induced Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
(1) Animals. A total of 30 healthy male New Zealand White (NZW) rabbits, weighing 2–2.2 kg, were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine [license number: SXCK (Shanghai) 2012-0008]. Basal body temperature (BBT) screening was conducted prior to the experiment.
(2) Major Agent. Bacterial endotoxin (Sigma, L2630, 10 mg), type I collagenase (Sigma, lot number: C0130), Masson staining kit (Beijing Reagan, lot number: DC0032), rabbit TNF-α kit (R&D Systems subpackage, rb201510200842), IL-1β kit (R&D Systems subpackage, rb201510190821), and IL-4 kit (R&D Systems subpackage, rb201510270951) were the major agents used in this study.
(3) Major Instruments. Pyrogen measurer (ZRY-3, Tianjin Tianda Tianfa), Upright Microscope (CX41, OLYMPUS), IMS Image Analysis System (Jrdun Biotechnology, Shanghai), Microplate Reader, and a spectrophotometer were used in this study.
+ Expand
(1) Animals. A total of 30 healthy male New Zealand White (NZW) rabbits, weighing 2–2.2 kg, were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine [license number: SXCK (Shanghai) 2012-0008]. Basal body temperature (BBT) screening was conducted prior to the experiment.
(2) Major Agent. Bacterial endotoxin (Sigma, L2630, 10 mg), type I collagenase (Sigma, lot number: C0130), Masson staining kit (Beijing Reagan, lot number: DC0032), rabbit TNF-α kit (R&D Systems subpackage, rb201510200842), IL-1β kit (R&D Systems subpackage, rb201510190821), and IL-4 kit (R&D Systems subpackage, rb201510270951) were the major agents used in this study.
(3) Major Instruments. Pyrogen measurer (ZRY-3, Tianjin Tianda Tianfa), Upright Microscope (CX41, OLYMPUS), IMS Image Analysis System (Jrdun Biotechnology, Shanghai), Microplate Reader, and a spectrophotometer were used in this study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!