Log In Sign up
The largest database of trusted experimental protocols

Anti sm22α

Manufactured by GeneTex
Visit Supplier

Anti-SM22α is a monoclonal antibody that specifically recognizes the SM22α protein. SM22α is a calponin-related, actin-binding protein that is involved in the regulation of smooth muscle cell differentiation and contractility. The Anti-SM22α antibody can be used for the detection and quantification of SM22α in various applications, such as Western blotting, immunohistochemistry, and flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Anti sma, by Merck Group (4 mentions) Protein assay kit, by Bio-Rad (3 mentions) Pvdf membrane, by Merck Group (3 mentions) Hrp conjugated secondary antibody, by Jackson ImmunoResearch (3 mentions) Anti actin, by Merck Group (3 mentions) Anti integrin α2, by Merck Group (2 mentions) Anti claudin 5, by Thermo Fisher Scientific (2 mentions) Anti occludin, by Thermo Fisher Scientific (2 mentions) Anti zo 1, by Thermo Fisher Scientific (2 mentions) Anti aqp4, by Merck Group (2 mentions) Anti myocardin, by R&D Systems (2 mentions) Mab4028, by Merck Group (2 mentions) Anti pdgfrβ, by Cell Signaling Technology (2 mentions) Abc kit, by Vector Laboratories (1 mentions) Dab kit, by Vector Laboratories (1 mentions) Anti nestin, by Merck Group (1 mentions) Anti collagen 1, by Merck Group (1 mentions) Anti ki67, by Merck Group (1 mentions) Anti fibronectin, by Merck Group (1 mentions) Anti collagen 4, by Merck Group (1 mentions)

4 protocols using anti sm22α

1

Immunophenotyping of Aortic Cell Populations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aortic sections were immunostained with anti-laminin γ1 (Abcam), anti-nestin (Sigma), anti-SMA (Sigma), anti-SM22α (GeneTex), anti-fibronectin (Millipore), anti-collagen I (Sigma), anti-collagen IV (Millipore), anti-CD90 (Abcam), anti-CD105 (Biolegend), anti-PDGFRα (eBiosciences), anti-c-Kit (R&D), and anti-Ki67 (Millipore) antibodies overnight at 4°C. For fluorescent staining, sections were incubated with appropriate fluorescent secondary antibodies (Invitrogen) for 1 hour at room temperature. Due to strong autofluorescence in the green channel of aortic tissue, Alexa-647 (artificially colored in green) rather than Alexa-488 was used. For DAB staining, sections were incubated with biotinylated secondary antibodies for 1 hour at room temperature, followed by ABC kit (Vector) and DAB Kit (Vector), according to the manufacturer’s instructions. After mounting, the sections were examined and photographed with Zeiss Axiovert 200 or Leica confocal microscope.
Yao Y., Norris E.H, & Strickland S. (2014). The cellular origin of laminin determines its role in blood pressure regulation. Cellular and molecular life sciences : CMLS, 72(5), 999-1008.
+ Open protocol
+ Expand
2

Quantitative Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein concentration from the lysates was determined using the Bio-Rad protein assay kit. Equal amount of protein was loaded and separated in 10% or 12% SDS-PAGE, followed by transfer to PVDF membrane (Millipore). The membranes were then incubated with anti-AQP4 (Millipore, AB3594, 1:500), anti-occludin (Invitrogen, 71-1500, 1:1000), anti-ZO-1 (Invitrogen, 40-2200, 1:1000), anti-Claudin-5 (Invitrogen, 34-1600, 1:1000), anti-PDGFRβ (Cell Signaling, 3169S, 1:500), anti-SMA (Sigma, F3777, 1:1000), anti-SM22-α (GeneTex, GTX113561, 1:1000), anti-integrin α2 (Millipore, AB1936, 1:500), anti-myocardin (R&D, MAB4028, 1:500) and anti-actin (Sigma, A5441, 1:2000) antibodies at 4 overnight, followed by incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Lab). The proteins were visualized by SuperSignal West Pico Chemiluminescent Substrate (Pierce). The density of bands was normalized to actin and quantified using NIH Image J.
Yao Y., Chen Z.L., Norris E.H, & Strickland S. (2014). Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nature communications, 5, 3413.
+ Open protocol
+ Expand
3

Quantitative Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein concentration from the lysates was determined using the Bio-Rad protein assay kit. Equal amount of protein was loaded and separated in 10% or 12% SDS-PAGE, followed by transfer to PVDF membrane (Millipore). The membranes were then incubated with anti-AQP4 (Millipore, AB3594, 1:500), anti-occludin (Invitrogen, 71-1500, 1:1000), anti-ZO-1 (Invitrogen, 40-2200, 1:1000), anti-Claudin-5 (Invitrogen, 34-1600, 1:1000), anti-PDGFRβ (Cell Signaling, 3169S, 1:500), anti-SMA (Sigma, F3777, 1:1000), anti-SM22-α (GeneTex, GTX113561, 1:1000), anti-integrin α2 (Millipore, AB1936, 1:500), anti-myocardin (R&D, MAB4028, 1:500) and anti-actin (Sigma, A5441, 1:2000) antibodies at 4 overnight, followed by incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Lab). The proteins were visualized by SuperSignal West Pico Chemiluminescent Substrate (Pierce). The density of bands was normalized to actin and quantified using NIH Image J.
Yao Y., Chen Z.L., Norris E.H, & Strickland S. (2014). Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nature communications, 5, 3413.
+ Open protocol
+ Expand
4

Molecular Profiling of Murine Aorta

Check if the same lab product or an alternative is used in the 5 most similar protocols
Descending thoracic aorta was dissected out from saline-perfused mice. After removing surrounding connective tissue, descending thoracic aorta was homogenized in tissue lysis buffer (100 mM Tris, pH 8, 1% SDS, 200 mM NaCl, 5 mM EDTA, 1X protease inhibitor cocktail, 1X phosphatase inhibitor cocktail). Total protein concentration from the lysates was determined using the Bio-Rad protein assay kit. Equal amounts of proteins were loaded and separated on 10% or 12% SDS-PAGE, followed by transfer to PVDF membrane (Millipore). The membranes were then incubated with anti-laminin γ1 (Neo Markers), anti-ATR1 (Abcam), anti-ATR2 (Abcam), anti-SM-calponin (Millipore), anti-SMA (Sigma), anti-SM22α (GeneTex), anti-nestin (Santa Cruz), or anti-actin (Sigma) antibodies at 4°C overnight, followed by incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch Lab). The proteins were visualized by SuperSignal West Pico Chemiluminescent Substrate (Pierce). Band density was normalized to actin and quantified using NIH Image J.
Yao Y., Norris E.H, & Strickland S. (2014). The cellular origin of laminin determines its role in blood pressure regulation. Cellular and molecular life sciences : CMLS, 72(5), 999-1008.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!