As SBF, Hanks’ balanced salt solution (HBSS) was prepared without organic species and adjusted pH = 7.4 [27 (link)]. Ti and ZrO2/Ti disks were immersed in 20 mL of HBSS at 37 °C in a polypropylene bottle. After immersing for 6 and 24 h, the excess HBSS on the disks was removed using soft paper, and the disks were immediately dried in a desiccator. The surfaces of the Ti and ZrO2/Ti disks were then observed using SEM at an accelerating voltage of 15 kV after sputter-coating with Au. The crystallographic structures of the precipitates on the Ti and ZrO2/Ti disks were analyzed using XRD (RINT-RAPID2 MM007; Rigaku, Tokyo, Japan), with an X-ray source of Cu-Kα at a power of 40 kV × 30 mA, and FT-IR (FT/IR-430; Jasco, Tokyo, Japan) using the KBr method. The precipitated products were detached from the Ti and ZrO2/Ti disks for FT-IR measurements.
Ft ir 430
Manufactured by Jasco
Sourced in Japan
The FT/IR-430 is a Fourier Transform Infrared (FT-IR) spectrometer. It is a core laboratory instrument used for the analysis and identification of chemical compounds through the detection and measurement of their infrared absorption spectrum.
Automatically generated - may contain errors
Lab products found in correlation
Tga sdta851, by PerkinElmer (1 mentions)
Jsm 6500lv, by JEOL (1 mentions)
Knowitall informatics system, by Bio-Rad (1 mentions)
Inova 500, by Agilent Technologies (1 mentions)
Gemini c18, by Phenomenex (1 mentions)
Lambda 7 spectrophotometer, by PerkinElmer (1 mentions)
713 ph meter, by Metrohm (1 mentions)
1100 series, by Agilent Technologies (1 mentions)
Agilent 6530 q tof mass spectrometer, by Agilent Technologies (1 mentions)
Smartlab xrd, by Rigaku (1 mentions)
Fluoview fv1000, by Olympus (1 mentions)
Tga sdta 851, by Mettler Toledo (1 mentions)
11 protocols using ft ir 430
1
Biomineralization on Ti and ZrO2/Ti
2
Structural Characterization of Cellulose and S-CSHE
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The chemical structures of cellulose and sulfonic-cellulose succinate half ester (S-CSHE) were characterized by FT-IR spectroscopy (FT/IR-430, JASCO, Tokyo, Japan). The samples were prepared by grinding to a fine powder (sample/KBr 1:1000 ratio). FT-IR spectra were recorded over the wavenumber range from 500 cm−1 to 4000 cm−1.
3
Characterization of GSA Catalyst
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FT-IR spectra of the GSA catalyst were recorded and qualitatively analyzed using (FT/IR-430, JASCO, Japan). Samples were prepared by grinding into fine powders with KBr. The spectrum was recorded in the range of 400–4000 cm−1.
Thermo-gravimetric analysis (TGA) measurement was conducted on a thermal analyzer TGA/SDTA 851 (Perkin-Elmer, USA). Samples (20 mg) were placed in corundum crucibles non-hermetically closed with lids, and heated under argon from room temperature to 400 °C at a heating rate of 5 °C min−1.
Field emission scanning electron microscopy (FE-SEM) images of the samples were obtained by scanning electron microscope (JEOL, JSM-6500 LV).
NH3-TPD technique was used to estimate the acidity of the GSA catalyst. Prior to NH3 adsorption, the catalyst was outgassed at 300 °C for 1 h. After cooling down to room temperature, NH3 adsorption was carried out under a flow of NH3/He (10 vol%, 50 mL min−1) and the temperature was maintained for 30 min. Then, the flow was switched to He (50 mL min−1) so as to remove the weakly adsorbed NH3. NH3-TPD was conducted in the He flow (50 mL min−1) by increasing the temperature to 800 °C with a heating rate of 5 °C min−1, while the desorbed NH3 molecules were detected using online mass spectrometry (Inficon quadrupole).
Thermo-gravimetric analysis (TGA) measurement was conducted on a thermal analyzer TGA/SDTA 851 (Perkin-Elmer, USA). Samples (20 mg) were placed in corundum crucibles non-hermetically closed with lids, and heated under argon from room temperature to 400 °C at a heating rate of 5 °C min−1.
Field emission scanning electron microscopy (FE-SEM) images of the samples were obtained by scanning electron microscope (JEOL, JSM-6500 LV).
NH3-TPD technique was used to estimate the acidity of the GSA catalyst. Prior to NH3 adsorption, the catalyst was outgassed at 300 °C for 1 h. After cooling down to room temperature, NH3 adsorption was carried out under a flow of NH3/He (10 vol%, 50 mL min−1) and the temperature was maintained for 30 min. Then, the flow was switched to He (50 mL min−1) so as to remove the weakly adsorbed NH3. NH3-TPD was conducted in the He flow (50 mL min−1) by increasing the temperature to 800 °C with a heating rate of 5 °C min−1, while the desorbed NH3 molecules were detected using online mass spectrometry (Inficon quadrupole).
4
Fourier Transform Infrared Spectroscopy Analysis
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Spectra of Flu, Gel, and F-AC0 were obtained by a Fourier transform-infrared spectrometer (FT-IR430, JASCO, Tokyo, Japan). FT-IR spectra were obtained from discs containing 1 mg sample in approximately 100 mg potassium bromide (KBr). The measuring was performed at a resolution of 1 cm -1 for 100 times iterations in the wavelength range from 4,000 to 400 cm -1 . The obtained spectra (1,600-1,500cm -1 ) were analyzed using a software (KnowItAll Informatics System, Bio-Rad, Hercules, CA, USA).
5
KBr Pellet FT-IR Spectroscopy
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FT-IR spectra were recorded as KBr pelletson JASCO FT/IR-430.
6
X-ray Photoelectron Spectroscopy and Thermal Desorption Analysis
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XPS spectra
were obtained with
a PHI 5600 multitechnique ESCA-Auger spectrometer equipped with a
monochromated Al Kα X-ray source. Analyses were carried out
with a photoelectron takeoff angle of 45° (relative to the sample
surface) with an acceptance angle of ±7°. The XPS binding
energy scale was calibrated by centering the C 1s peak due to hydrocarbon
moieties and “adventitious” carbon at 285.0 eV.
FTIR spectra were obtained with JASCO FTIR 430, using 100 scans per
spectrum (scan range 560–4000 cm–1, resolution
4 cm–1).
Thermal desorption experiments were
performed in a UHV chamber
(basic pressure ∼ 10–8 Torr). For the experiments,
the holder (Vacuum Science, Italy) was resistively heated with a ramp
of about 10 °C/min from 30 to 200 °C. The desorbed molecules
were detected with a Smart-IQ + (Thermo Electron Corporation) quadrupole
mass spectrometer equipped with an electron filament as ion source
and a multiplier detector (mass range 1–300).
were obtained with
a PHI 5600 multitechnique ESCA-Auger spectrometer equipped with a
monochromated Al Kα X-ray source. Analyses were carried out
with a photoelectron takeoff angle of 45° (relative to the sample
surface) with an acceptance angle of ±7°. The XPS binding
energy scale was calibrated by centering the C 1s peak due to hydrocarbon
moieties and “adventitious” carbon at 285.0 eV.
FTIR spectra were obtained with JASCO FTIR 430, using 100 scans per
spectrum (scan range 560–4000 cm–1, resolution
4 cm–1).
Thermal desorption experiments were
performed in a UHV chamber
(basic pressure ∼ 10–8 Torr). For the experiments,
the holder (Vacuum Science, Italy) was resistively heated with a ramp
of about 10 °C/min from 30 to 200 °C. The desorbed molecules
were detected with a Smart-IQ + (Thermo Electron Corporation) quadrupole
mass spectrometer equipped with an electron filament as ion source
and a multiplier detector (mass range 1–300).
7
Comprehensive Spectroscopic Characterization
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Nuclear magnetic resonance (NMR) spectra were recorded on a Varian Inova-500 instrument operating at 499.6 and 125.6 MHz for 1 H and 13 C, respectively, and referenced with deuterated solvents (CDCl3 or CD3OD). The carbon multiplicity was evidenced by distortion-less enhancement by polarisation transfer (DEPT) experiments. The proton couplings were evidenced by 1 H-1 H COSY experiments. The heteronuclear chemical shift correlations were determined by HMQC and HMBC pulse sequences.
IR spectra were recorded on a Jasco FT/IR-430 instrument equipped with single reflection ATR using CHCl3 as solvent.
UV-Vis spectra were recorded with a Varian Cary 300 UV-Vis spectrophotometer or on a PerkinElmer Lambda 7 spectrophotometer. pH was measured with a Metrohm combined glass electrode, connected to a Metrohm 713 pHmeter.
HPLC-UV was an Agilent 1100 Series binary pump HPLC equipped with a UV detector set at 254 nm. The column used was a Gemini C18 (5µm, 250 mm x 4.6 mm, Phenomenex) with a flow rate of 0.4 mL min -1 . The mobile phase was: water with 0.1% formic acid (A) and acetonitrile (B). The gradient elution was: at initial time B 30 % and A 70%, followed by a linear gradient to B 75 % within 55 min, the ratio was maintained constant for 5 min, then the ratio returned to B 30% in 5 min.
IR spectra were recorded on a Jasco FT/IR-430 instrument equipped with single reflection ATR using CHCl3 as solvent.
UV-Vis spectra were recorded with a Varian Cary 300 UV-Vis spectrophotometer or on a PerkinElmer Lambda 7 spectrophotometer. pH was measured with a Metrohm combined glass electrode, connected to a Metrohm 713 pHmeter.
HPLC-UV was an Agilent 1100 Series binary pump HPLC equipped with a UV detector set at 254 nm. The column used was a Gemini C18 (5µm, 250 mm x 4.6 mm, Phenomenex) with a flow rate of 0.4 mL min -1 . The mobile phase was: water with 0.1% formic acid (A) and acetonitrile (B). The gradient elution was: at initial time B 30 % and A 70%, followed by a linear gradient to B 75 % within 55 min, the ratio was maintained constant for 5 min, then the ratio returned to B 30% in 5 min.
8
Spectroscopic and Microscopic Analysis of Adsorption
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The materials were characterized by Fourier Transform infrared spectroscopy (79 Jasco FTIR 430) to measure IR spectra in a spectral range of 4000–400 cm−1; a resolution of 4 cm−1, and a scanning speed of 2 mm s−1. And also by electron microscopy before and after the adsorption process.
9
FTIR Characterization of Copolymer Resins
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Control and the copolymer resin groups were characterized by FTIR spectroscopy (FT/IR-430, JASCO, Tokyo, Japan). Polymerized specimens were dried using a dry-heat oven for 12 h at 70°C to eliminate H 2 molecules from the system. The specimens were cut into small pieces. One milligram sample size was used for each test group to acquire a clear FTIR spectrum.
10
Isolation and Identification of Broussoflavonol B
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The dried stem bark of Broussonetia kazinoki Siebold (2 kg) was extracted with 70% ethanol with reflux for 2 h and evaporated to generate extracts. The concentrated extracts (90 g) were suspended in water and partitioned with ethyl acetate. The ethyl acetate soluble fraction (20 g) was chromatographed over silica gel column with n-hexane/ethyl acetate gradient elution (50:1→1:1, v/v) to obtain 15 fractions. Subsequently, fraction 9 (503 mg) was subjected to column chromatography on silica gel eluting with methylene chloride/methanol (100:1→1:5, v/v) to give 10 fractions. Subfraction F9-3 (170 mg) was further separated on a RP-C18 column with a gradient elution of acetonitrile (40%→100%) to yield broussoflavonol B (34 mg). The structure of broussoflavonol B was identified by spectroscopic analyses of NMR (Varian INOVA 400 MHz, Varian Inc., Palo Alto, CA, USA), Mass (Agilent 6530 Q-TOF mass spectrometer, Agilent, Santa Clara, CA, USA) and IR (FT/IR-430, Jasco Inc., Tokyo, Japan) [47 (link)].
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