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Hif 1α antibody

Manufactured by Cayman Chemical
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Sourced in United States

The HIF-1α antibody is a laboratory reagent used for the detection and quantification of HIF-1α, a transcription factor that plays a crucial role in the cellular response to hypoxia. This antibody can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and ELISA, to study the expression and localization of HIF-1α in biological samples.

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Lab products found in correlation

Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (2 mentions) Gapdh antibody, by Cell Signaling Technology (2 mentions) Ripa lysis and extraction buffer, by Thermo Fisher Scientific (2 mentions) Pygl antibody, by Merck Group (2 mentions) Pygb antibody, by Proteintech (2 mentions) Complete ultra protease phosphatase inhibitor, by Roche (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Hif 2α antibody, by Novus Biologicals (2 mentions) Nel103001ea, by PerkinElmer (2 mentions) Apoptosis detection kit, by BD (1 mentions) Cell counting kit 8 assay kit, by Keygen Biotech (1 mentions) Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions) Tunel brightgreen apoptosis detection kit, by Vazyme (1 mentions) Goat anti rabbit hrp, by Thermo Fisher Scientific (1 mentions) Goat anti mouse hrp, by Thermo Fisher Scientific (1 mentions) Odyssey clx imager, by LI COR (1 mentions) Amersham ecl, by GE Healthcare (1 mentions) Irdye 800cw donkey anti mouse, by LI COR (1 mentions) Irdye 680rd donkey anti rabbit, by LI COR (1 mentions)

Close spelling variants of this product

HIF-1α (28 citations) Anti-HIF-1α antibody (3 citations) HIF-1α (C-Term) Polyclonal Antibody (2 citations)

4 protocols using hif 1α antibody

1

Investigating Oxidative Stress and Apoptosis

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HIF-1α antibody (No10006421, 1:200) and TBARS (MDA) Assay Kit (No 10009055) were bought from Cayman chemical (Ann Arbor, MI, United States). Antibodies of Bax (No 2772) was purchased from Cell Signaling Technology (Danvers, MA, United States). Anti-SOD2 antibody (No ab13533) was provided by Abcam (Cambridge, United Kingdom). Bcl-2(No 26593-1-AP) and β-actin (No 60008-1-Ig) were bought from Proteintech, (Rosemont, IL, United States). Doxorubicin (No D1515) was purchased from sigma (Saint Louis, MO, United States). FG-4592 (No S1007) was from Selleck Chemicals IIc (Houston, TX, United States). MitoSOX™ Red Mitochondrial Superoxide Indicator (No M36008) was purchased from ThermoFisher Scientific (Waltham, MA, United States). Cell Counting Kit-8 assay kit (No KGA317) was from KeyGen Biotech (Nanjing, China). TNF-α (No DKW12-2720-096) and IL-6 (No DKW12-2060-096) ELISA kits were purchased from Dakewe Biotech (Shenzhen, China). Apoptosis detection kits (No 556547 and No 559763) were from BD Biosciences (San Diego, CA, United States). TUNEL BrightGreen Apoptosis Detection Kit (No A112-01) was bought from Vazyme Biotech (Nanjing, China).
Long G., Chen H., Wu M., Li Y., Gao L., Huang S., Zhang Y., Jia Z, & Xia W. (2020). Antianemia Drug Roxadustat (FG-4592) Protects Against Doxorubicin-Induced Cardiotoxicity by Targeting Antiapoptotic and Antioxidative Pathways. Frontiers in Pharmacology, 11, 1191.
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2

Western Blot Analysis of Glycolytic Proteins

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Cells and tumor tissue samples were lysed in RIPA lysis and extraction buffer (cat. 89900, ThermoFisher Scientific) containing Roche complete ULTRA Protease/Phosphatase Inhibitor (cat. 05892791001). Protein concentration was quantified with Pierce BCA Protein Assay Kit (cat. 23225, ThermoFisher Scientific). Isolated proteins were resolved by SDS-PAGE, and Western blot analysis was performed. All primary antibodies were diluted 1:1,000 in 5% w/v nonfat milk. Blots were incubated with primary antibodies overnight at 4°C. GYS1 (cat. ab40810) and ACTIN (cat. ab3280) antibodies were from abcam; HIF-1α antibody was from Cayman (cat. 10006421); HIF-2α antibody was from Novus Biologicals (cat. NB100-122); GAPDH antibody was from Cell Signaling Technology (cat. 2118); PYGL antibody was from Sigma (cat. HPA000962); PYGB antibody was from proteintech (cat. 12075-1-AP). Primary antibodies were detected using horseradish peroxidase–conjugated secondary antibodies from Cell Signaling Technology (cat. 7074) followed by exposure to enhanced chemiluminescence substrate (cat. NEL103001EA, PerkinElmer) or SuperSignal West Femto Maximum Sensitivity Substrate (cat. 34095, ThermoFisher Scientific).
Xie H., Song J., Godfrey J., Riscal R., Skuli N., Nissim I, & Simon M.C. (2021). Glycogen metabolism is dispensable for tumor progression in clear cell renal cell carcinoma. Nature metabolism, 3(3), 327-336.
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3

Western Blot Analysis of HIF-1α and Arginine Metabolism

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Protein samples were separated on 4–12% Bis-Tris acrylamide gels (Life Technologies) and transferred to PVDF (Millipore). HIF-1α antibody was purchased from Cayman Chemical (10006421) and Novus Bio (NB100-479). Arginase I (H-52) and NOS2 (M19) antibodies were purchased from Santa Cruz Biotechnology. Rabbit anti-β-actin (A2066) was purchased from Sigma. β-tubulin (AA2) was purchased from Millipore. Secondary antibodies were goat anti-rabbit HRP (Thermo), goat anti-mouse HRP (Thermo), donkey anti-mouse IRDye800CW, donkey anti-goat IRDye680RD and donkey anti-rabbit IRDye680RD (Licor). Blots were developed with Supersignal Femto (Thermo) or Amersham ECL (GE Healthcare Life Sciences) or visualized on an Odyssey CLx imager (Licor).
Tran C.W., Gold M.J., Garcia-Batres C., Tai K., Elford A.R., Himmel M.E., Elia A.J, & Ohashi P.S. (2020). Hypoxia-inducible factor 1 alpha limits dendritic cell stimulation of CD8 T cell immunity. PLoS ONE, 15(12), e0244366.
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4

Western Blot Analysis of Glycolytic Proteins

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Cells and tumor tissue samples were lysed in RIPA lysis and extraction buffer (cat. 89900, ThermoFisher Scientific) containing Roche complete ULTRA Protease/Phosphatase Inhibitor (cat. 05892791001). Protein concentration was quantified with Pierce BCA Protein Assay Kit (cat. 23225, ThermoFisher Scientific). Isolated proteins were resolved by SDS-PAGE, and Western blot analysis was performed. All primary antibodies were diluted 1:1,000 in 5% w/v nonfat milk. Blots were incubated with primary antibodies overnight at 4°C. GYS1 (cat. ab40810) and ACTIN (cat. ab3280) antibodies were from abcam; HIF-1α antibody was from Cayman (cat. 10006421); HIF-2α antibody was from Novus Biologicals (cat. NB100-122); GAPDH antibody was from Cell Signaling Technology (cat. 2118); PYGL antibody was from Sigma (cat. HPA000962); PYGB antibody was from proteintech (cat. 12075-1-AP). Primary antibodies were detected using horseradish peroxidase–conjugated secondary antibodies from Cell Signaling Technology (cat. 7074) followed by exposure to enhanced chemiluminescence substrate (cat. NEL103001EA, PerkinElmer) or SuperSignal West Femto Maximum Sensitivity Substrate (cat. 34095, ThermoFisher Scientific).
Xie H., Song J., Godfrey J., Riscal R., Skuli N., Nissim I, & Simon M.C. (2021). Glycogen metabolism is dispensable for tumor progression in clear cell renal cell carcinoma. Nature metabolism, 3(3), 327-336.
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